ACCLAIM oa - Cromlab

Lot Qualification TestsColumn Performance Test, Acclaim SurfactantIsocratic Surfactant SelectivityEach column is tested under these conditions to guarantee thatthe column is well manufactured. Column pressure, efficiency,asymmetry and retention time of propylbenzene are specifiedfor each column. This test also is used to monitor batch-to-batchreproducibility as measured by the relative retention of an anionicsurfactant to a neutral marker.Mobile Phase: 50:50 (v/v) acetonitrile : 0.1M NH 4 OAc, pH 5.2Flow Rate: 1.0 mL/minTemperature: 30 °CInj. Volume: 5 µLDetection: UV, 220 nmPeaks: 1. Uracil 0.10 mg/mL2. 2-(4-octylphenoxy)ethoxyethyldimethylbenzylammonium chloride 0.10 mg/mL3. Sodium p-Toluenesulfonate 0.20 mg/mL4. Propylbenzene 0.40 mg/mLColumn Performance Test, Acclaim Explosives E1 and E2Each column is tested under these conditions to guarantee thatthe column is well manufactured. Column pressure, efficiency,asymmetry, and retention time of naphthalene are specified foreach column.Mobile Phase: 70/30 v/v acetonitrile/waterFlow Rate: 1.0 mL/minTemperature: 30 °CInj. Volume: 5 µLDetection: UV, 254 nmPeaks: 1. Uracil 0.05 mg/mL2. Naphthalene 0.5 mg/mLGradient Surfactant ResolutionAn application-based test for the resolution of anionic, nonionicand cationic surfactants.Mobile Phase A: AcetonitrileMobile Phase B: 0.10 M Ammonium acetate, pH 5.4Time %A %B0.0 25 7525.0 85 1530.0 85 15Flow Rate: 1.0 mL/minTemperature: 30 °CInj. Volume: 20 µLDetection: Evaporative Light ScatteringAnalytes: 1. Laurylpyridinium chloride 0.10 mg/mL2. Lauryldimethylbenzylammonium chloride 0.10 mg/mL3. Triton X-100 0.40 mg/mL4. Sodium decylsulfate 0.20 mg/mL5. Sodium dodecylsulfate 0.10 mg/mLAPPENDIXwww.dionex.com - 51 -