ACCLAIM oa - Cromlab

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ApplicationsPa r t Th r e e: ApplicationsContentsAPPLICATIONSPharmaceuticalIsocratic Resolution of Antihistamines and TheirImpurities on Acclaim 120 Å, C18............................ 29Separation of Four Benzodiazepine Drugson Acclaim 120 C18..................................................... 29Separation of Five Antidepressant onAcclaim PA................................................................... 29Spectinomycin and Lincomycin on Acclaim PAwith Electrochemical Detection................................. 29Beta Blockers on Acclaim PA2.................................. 29Venlafaxine and Related Substances........................ 29Assay for Pramoxine in Topical Anesthetic Ointmenton Acclaim 120 Å, C18 with SPE Cleanup onOnGuard II RP.............................................................. 30Determination of Four Active Ingredients in aMultisymptom Cold Remedy Using Acclaim PA. 30Separation of Basic Drugs on Acclaim 120 Å, C18....30Baseline Separation of Catecholamines & Serotoninat Physiological Concentrations on Acclaim120 Å, C18 with Electrochemical Detection............ 30Gradient Resolution of Sulfa Drugs onAcclaim PA................................................................... 30Catechol<strong>oa</strong>mines in Urine on Acclaim PA2............ 30Bromide and Organic Acids in Cough SyrupUsing Acclaim OA....................................................... 31Isocratic Separation of Six Cardiac Anti-Arrhythmic Drugs (Beta-Blockers) on 120 Å C8.... 31Sulfa Drugs on Acclaim PA2..................................... 31Active Ingredients in Zantac 75 Acid-ReducerMedication..................................................................... 31Loratidine and Pseudoephedrine on Acclaim PA. 31Propafenone and Related Substances...................... 31Antidepressants on Acclaim PA2............................. 32Active ingredients in Cough Syrup.......................... 32Barbiturates on Acclaim 120 C18.............................. 32Budesonide and Related Substances........................ 32Hydrocortisone in Skin Ointment............................ 32Phenytoin and Related Substances........................... 32Active Ingredients in Sunscreen Lotion onAcclaim 120 Å, C18 Using ASE Extraction............. 33Glucocorticosteroids in Serum on Acclaim 120 C18.33Nonsteroidal Anti-inflammatory Drugs.................. 33Fexofenadine & Pseudoephedrine on Acclaim PA...33EnvironmentalLC/MS Detection of Herbicides onAcclaim 120 C18........................................................... 34LC Separation of Nitrosamines in EPA 8270Using Acclaim PA........................................................ 34Benzidines by EPA Method 605 on Acclaim120 C18 with Electrochemical Detection................. 34Phenol Standards by EPA Method 604 onAcclaim PA................................................................... 34Separation of Phthalate Esters in EPAMethod 606 Using Acclaim PA................................. 34Brodifacoum in Rodent Bait...................................... 34Thirteen Carbonyl-DNPH Derivatives forCARB Method 1004 on Acclaim 120 Å, C18........... 35Carbamate Insecticides in EPA 531.1 onAcclaim 120 C8 with MS Detection.......................... 35Improved Separation of 13 Carbonyl-DNPHDerivatives by CARB Method 1004 UsingCoupled Acclaim PA and Acclaim C18Columns......................................................................... 35Polynuclear Aromatic Hydrocarbon Standardsby EPA Method 8310 on Acclaim PA..................... 35LC Separation of Benzidines in EPA 8270 UsingAcclaim PA................................................................... 36Sensitive Detection of Triazine Herbicides onAcclaim 120 C18 with MS Detection........................ 36EPA Method 8330 Using Acclaim Explosives E2.. 36EPA Method 8330 Using Acclaim Explosives E1.. 36Four Antibiotics in Animal Feed onAcclaim 120 C18........................................................... 36Carbamate Insecticide Standards in EPA 531.1 onAcclaim PA................................................................... 36Food and BeverageFat-Soluble Vitamin Standards onAcclaim 120 C18........................................................... 37Amino Acids in Vitamin Premix on Acclaim OA.. 37Organic Acids in Orange Juice on Acclaim OA..... 37Organic Acids in White Wine on Acclaim OA...... 37Carotenoids in Serum on Acclaim PA..................... 37Pigments in Turmeric on Acclaim PA2................... 37Assay of Aspartame and Its Impurities on ThreeFormats of Acclaim 120 C18 Columns..................... 38Assay for Water-Soluble Vitamins in VitaminTablets Using Acclaim PA.......................................... 38Nitrofuran Antibiotic Residues in Animal Feed.... 38Fat-Soluble Vitamins and Carotenoids inVitamin Tablets............................................................ 38Determination of Quinine in Tonic Water.............. 39Determination of Ingredients of an Energy Drink. 39Hops Extract on Acclaim 120 C18............................ 39Sudan Dyes in Paprika............................................... 39Water-Soluble Vitamins Using Acclaim PA2......... 39Parabens on Acclaim PA2.......................................... 39Four Aflatoxin Standards on Acclaim 120 C18...... 40Fat-Soluble Vitamin Standards UsingAcclaim PA................................................................... 40Glutamate in Beer by Automated Precolumn OPADerivatization............................................................... 40Determination of Soft Drink Ingredients................ 40Tetracycline Antibiotic Residues in Pork................ 40Fat-Soluble Vitamins and Carotenoids in DryPet Food......................................................................... 40Antioxidants in Edible Oils by AOAC 983.15 onAcclaim 120 C18........................................................... 41Sulfonamide Anibiotic Residues in Milk................. 41Bitter Principles in Beer on Acclaim 120 C18......... 41ChemicalSeparation of Cationic Surfactants Using ELSD.... 42Separation of Ethoxylated Quats.............................. 42Analysis of ZONYL FSO Fluorosurfactant............. 42Analysis of a Shampoo............................................... 42Analysis of a Fabric Softener..................................... 42Analysis of a Mouthwash........................................... 42Separation Hydrotropes, Anionic, Cations, andAmphoteric Surfactiants............................................. 43Analysis of Sodium Lauryl Sulfate UsingLC-ESI-MS..................................................................... 43Analysis of PEG monoethyl ether (MW-550)......... 43Analysis of Triton X-100 Using LC-ESI-MS............ 43Analysis of a Laundry Washing Detergent............ 43Analysis of a Dishwashing Liquid........................... 43Analysis of Ammonium Laureth Sulfate UsingConductivity Detection............................................... 44Separation of Inorganic Anion, Hydrotropes,Cations, Nonionic, Amphoteric and AnionicSurfactiants.................................................................... 44Analusis of Toilet Bowl Cleaner............................... 44Analysis of a Strongly Hydrophilic Hydrotrope... 44Analysis of Quats Using LC-ESI-MS........................ 44Analysis of NEODOL 25-7......................................... 44Analysis of PEG-200 Distearate................................. 45Separation of Cationic Surfactants withSuppressed Conductivity Detection......................... 45Separation of Quaternary ImidazoliniumCompounds................................................................... 45Separation of Different Polyethylene Glycols........ 45POE(30) Ammonium Lauryl Sulfate........................ 45Sudan Dyes on Acclaim 120 C18.............................. 45BioscienceTryptic Peptide Map of Cytochrome con Acclaim 300 C18..................................................... 46Capillary LC for Peptide Mapping UsingAcclaim 300 C18.........................................................46Separation of Protein Standards by Micro-LC onAcclaim 300 C18........................................................... 46Tryptic Peptide Map of Bovine Serum Albuminon Acclaim 300 C18..................................................... 46Cytochrome c from Three Species onAcclaim 300 C18........................................................... 47Protein Mixture by Reversed-Phase HPLC onAcclaim 300 C18........................................................... 47Peptide Test Mix on Acclaim 300 and PA2 withFormic Acid................................................................... 47Comparison of Acid Additives for PeptideAnalysis......................................................................... 47Natural ProductsAlkaloids in Goldenseal Root onAcclaim 120 C18 with ASE Extraction..................... 48Isoflavones in Red Clover on Acclaim 120 C18with ASE Extraction.................................................... 48Flavonoids in Green and Black Teas UsingAcclaim 120 C18........................................................... 48Organic AcidsResolution of Benzene Polycarboxylic Acids onAcclaim OA................................................................... 49Acrylic Acid Oligomers on Acclaim OA................. 49Separation of Hydroxybenzoic Acids onAcclaim OA................................................................... 49- 28 -www.dionex.com
Pharmaceutical ApplicationsPharmaceutical12mAU01Isocratic Resolution of Antihistaminesand Their Impurities on Acclaim 120 C1820 2 4 6 8Minutes341012 14Column: Acclaim 120 C18, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) 50 mM sodium acetate(B) MethanolIsocratic: (A) 20%, (B) 80%Temperature: 25 °CDetection: UV, 249 nmPeaks: 1. Thenyldiamine HCI2. Phenothiazine3. Promethazine HCI4. Pyrrobutamine phosphate175100.18AU12345 60 5 10MinutesBeta Blockers on Acclaim PA278Column: Acclaim PA2, 5 µmDimensions: 4.6 × 150 mmMobile Phase: MeOH/0.2% NH 4 OH, pH10, v/v, 60/40Temperature: 30 °CFlow Rate: 1.0 mL/minInj. Volume: 5 µLDetection: UV, 210 nmPeaks: (40 ppm each)1. Maleate2. Labetalol3. Metaraminol4. Atenolol155. Acebutolol6. Metoprolol7. Timolol8. Oxprenolol21134Separation of Four Benzodiazepine Drugs on Acclaim 120 C18200mAU01230 1 2 3 4 5 6 7 8 9 10Minutes4Column: Acclaim 120 C18, 5 µmDimensions: 4.6 × 150 mmMobile Phase: 60% acetonitrileFlow Rate: 1.0 mL/minDetection: UV, 235 nmPeaks: 1. Oxazepam2. Clonazepam3. Temazepam4. Diazepam17511350mAU0Separation of Five Antidepressants on Acclaim PA120 1 2 3 4 5 6 7 8Minutes345Column: Acclaim PA, 5 µmDimensions: 4.6 × 150 mmMobile Phase: 80/20 v/v MeOH/30 mM phosphate, pH 6.0Temperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 5 µLDetection: UV, 220 nmPeaks: 1. Protriptyline 502. Nortriptyline 253. Doxepin 504. Imipramine 405. Amitriptyline 5019299700nC0–1000Spectinomycin and Lincomycin on Acclaim PAwith Electrochemical Detection51013215 20Minutes42553035Column: Acclaim PA, 3 µmDimensions: 2.1 × 150 mmMobile Phase (A) 10 mM acetic acid,3.3 g/L pentanesulfonic acid,pH 4.0 with NaOH(B) 10 mM acetic acid,0.55 g/L pentanesulfonic acid,pH 4.0 with NaOH(C) AcetonitrileGradient: Time % A % B % C0.0 70 30 010.0 70 30 010.1 0 100 020.0 0 100 023.0 0 75 2537.0 0 75 25Flow Rate: 0.30 mL/minTemperature: 40 °CInj. Volume: 10 µLDetection: Postcolumn addition of0.5 M NaOH at 0.12 mL/min;pulsed amperometric detectionPeaks: 1. Benzyl alcohol2. Spectinomycin anomer 13. Spectinomycin anomer 24. Spectinomycin5. Lincomycin800mAU01234Venlafaxine and Related Substances5 6 70 10 20 30 40 50MinutesData courtesy of: V. Bhate of Analytical Solutions89 10 11Column: Acclaim 120 C18, 5 µm,4.6 × 250 mmPump: Summit P680AMobile Phase: (A) 10% acetonitrile; 90% water;1.0 mL/L triethylamine,adj. to pH 3.5 with phosphoric acidMobile Phase: (B) 75% acetonitrile; 25% water;1.0 mL/L triethylamine,adj. to pH 3.5 with phosphoric acidFlow: 1.0 mL/minGradient Times: 0 35 40 45 47 55%A: 95 25 10 10 95 95%B: 5 75 90 90 5 5Temperature: TCC100 thermostat at 35 °CInjection: ASI-100 autosampler; 20 µLDetector: UVD 340U; UV at 226 nmSample: “Resolution” test mixPeaks: 1. Impurity 1 10 µg/mL2. Impurity A 103. FL II 104. Venlafaxine 10005. Impurity III 106. Impurity C 107. Impurity B1 108. PMPA 109. Impurity IV 1010. FL I 1011. Impurity V 10APPLICATIONS2038422660www.dionex.com - 29 -
Page 2: Acclaim 120Acclaim 300Acclaim PA/PA
Page 5 and 6: IntroductionAcclaim General Purpose
Page 8 and 9: IntroductionINTRODUCTIONUnderstandi
Page 10 and 11: IntroductionINTRODUCTIONAcclaim Col
Page 12: Acclaim 120 C18120 C18Pa r t Tw o:
Page 22 and 23: Acclaim OAChapter 6: Acclaim OA for
Page 24 and 25: Acclaim SurfactantChapter 7: Acclai
Page 26 and 27: Acclaim ExplosivesChapter 8: Acclai
Page 30 and 31: Pharmaceutical ApplicationsAU10 1 2
Page 32 and 33: Pharmaceutical Applications00-24 mA
Page 34 and 35: Environmental ApplicationsEnvironme
Page 36 and 37: Environmental Applications0.5 Colum
Page 38 and 39: Food and Beverage ApplicationsAssay
Page 41 and 42: Food and Beverage ApplicationsSulfo
Page 43 and 44: Ch e m i c a l ApplicationsSurfacta
Page 45 and 46: Ch e m i c a l ApplicationsSurfacta
Page 47: Bioscience ApplicationsProteins and
Page 51 and 52: Lot Qualification TestsColumn Perfo
Page 53 and 54: Part Number IndexAppendix 3:Part Nu
Page 55 and 56: Analyte Indexcis-Lycopene..........

ACCLAIM oa - Cromlab

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