ACCLAIM oa - Cromlab

Acclaim 120 C18120 C18Pa r t Tw o: Co l u m n sChapter 1: Acclaim 120 C18High performance reversed-phase columns for theseparation of small moleculesThe Acclaim 120 C18 series columns feature a densely bondedmonolayer of octadecyldimethylsiloxane on a highly pure, spherical,silica substrate with 120 Å pore structure.The Acclaim 120 C18 columns are the classic reversed-phasecolumns. These columns are recommended for general-purposereversed-phase applications where high surface coverage, lowsilanol activity, and excellent efficiency are required. The Acclaim120 C18 columns feature:• Highly efficient, symmetrical peaks for difficult basicand chelating analytes• Ultrapure silica substrate• Optimized surface pretreatment, proprietary highdensitybonding process, and double endcapping• Reliability designed into the manufacturing processand assured by thorough and appropriate testing• High hydrophobicity and low polarity yield highselectivity for hydrophobic substances• LC/MS compatible• Wide range of applications in pharmaceutical,environmental, food testing, and product-qualitytesting for small moleculesBondingUSP codeEndcappingC18 monomericL1Double% C 17.5–18.9Pore sizeCH 3Si CH 2 (CH 2 ) CH 3CH 3O 16Physical Specifications120–140 ÅSurface area 290–320 m 2 /gParticle diameter 2.9–3.2 or 4.2–4.5 µmPerformance SpecificationsLot Polar selectivity ratio 0.12–0.16Base asymmetry0.98–1.50 (5 µm)0.98–1.60 (3 µm)Metal activity ratio 0.90–1.30Steric selectivity ratio 1.58–1.65Column Efficiency (N/m) 90,000 (5 µm)120,000 (3 µm)Asymmetry (EP) 0.98–1.20Retention time8.41–9.29 (5 µm)8.31–9.19 (3 µm)Chromatographic specifications for Acclaim 120 C18 4.6 × 150 mm columns.Please refer to Part 4 for a detailed description of the tests and specifications.Specifications are subject to change at the discretion of Dionex.018-01-062018-01-060018-01-058018-01-056018-01-054018-01-046b018-01-046a018-01-032018-01-020Control Chart for Acclaim 120 C18 5 µm0 1 2 3 4 5 6 7k'Control chart showing k’ (phenanthrene) for nine lots of Acclaim 1205-µm C18. The data show the average and standard deviation for allcolumns shipped from that lot.54321LC-MS of Pesticides23028Column: Acclaim 120 C18, 3 µmDimensions: 2.1 × 100 mmMobile Phase: 70/30% methanol/0.1% formic acidFlow rate 0.25 mL/minDetection: AQA MS, +ESI, 30V, 200 °C,SIM as indicatedm/z mass injectedPeaks: 1. Monuron 199 1 ng2. Altrazine 216.1 1 ng3. Propazine 230 2 ng4. Diuron 233 2.5 ng5. Linuron 249 2 ngRecommended Ranges of OperationTo maintain wetting of the hydrophobic surface, the recommended range fororganic composition is 20–100%. The type 316 stainless steel column hardwareis compatible with common HPLC mobile phases. To prevent corrosion, highconcentrations of halide ions at pH