ACCLAIM oa - Cromlab

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Acclaim OAChapter 6: Acclaim OA forOrganic AcidsOptimized and application-tested for the analysis ofhydrophilic organic acidsAcclaim OA organic acid columns are special reversed-phasesilica columns, designed for the separation of aliphatic and aromaticorganic acids at low pH with UV-Visible detection. TheAcclaim OA uses a patented polar-embedded stationary phasethat allows a br<strong>oa</strong>d range of operating conditions, including 100%aqueous mobile phases.The Acclaim OA is the recommended column for determiningsmall hydrophilic organic acids, C1 to C7 aliphatic acids, andhydrophilic aromatic acid and is also valuable for the analysis andquality assurance of food and beverage products, pharmaceuticalpreparations, plating baths for semiconductor manufacturing,manufacturing chemicals, and chemical intermediates.The Acclaim OA columns are packed in metal-free PEEK columnbodies, to eliminate unwanted interactions between the analytesand the column body. Benefits of the Acclaim OA columnsinclude:• Use-tested to guarantee consistent organic acidseparations• Compatible with 100% aqueous mobile phases, optimumfor reversed-phase retention of hydrophilic aliphaticorganic acids• Hydrolytic stability at low-pH conditions, optimumfor reversed-phase retention of organic acids• Ideal selectivity for separating a wide spectrum oforganic acids, including hydrophilic aliphatic acids,C1 to C7 aliphatic acids, aromatic acids, and selectedamino acids• Excellent peak shapes for organic acids• High reversed-phase capacity with retention similar tostandard C18 phases• Suitable for analysis of foods, beverages, pharmaceuticals,chemical intermediates, and environmentalsamples• Ideal for quality assurance offering fast run times andminimal sample preparationOARecommended Ranges of OperationpH Range 2.0–8.0Pressure limit 4500 psi (2000 psi above 50%organic solvent content)Organic solventsHydrophilic Organic AcidsHO OHO OO OHOO OHOHOOH OHOHOO OHH OHHOOOHOHO1. Oxalic acid 2. Tartaric acid 3. Formc acid 4. Malic acid 5. iso-Citric acidOHOOO OHOOOHOH OH OHH OHOHO3 CH 3 COHHOOOOHO6. Lactic acid 7. Acetic acid 8. Citric acid 9. Succinic acid 10. Fumaric acidHOOO O O OOH OHOH OH11. cis-Acontic acid 12. trans-Aconitic acidOOH0–100% acetonitrile or methanol19511Surface chemistryUSP codeEndcappingPore sizePhysical SpecificationsProprietarynaYes120–140 ÅSurface area 290–320 m 2 /gParticle diameter 4.2–4.5 µmGuaranteed Performance for Organic AcidsThe performance of the Acclaim OA columns is guaranteedfor the separation of aliphatic and aromatic organic acids at lowpH. These columns undergo extensive testing to ensure columnto-columnreproducibility, and are shipped with quality assurancereports detailing these tests. The Acclaim OA columns are usetestedfor two specific applications—isocratic and gradient.These operating conditions provide a good starting point formethods development.Performance SpecificationsLot Metal activity ratio 0.80–1.50Gradient organic acids Baseline drift
Acclaim OAIsocratic Separation of Hydrophilic Organic AcidsRuggedness Test at pH 2.680.025AU021 3 45 6 7 8 91011120 5 10 15MinutesColumn: Acclaim OA, 5 µmDimensions: 4 × 250 mmMobile Phase: 100 mM Na 2 SO 4 , pH 2.65 (adjustedwith methanesulfonic acid)Temperature: 30 °CFlow Rate: 0.6 mL/minInj. Volume: 5 µLDetection: UV, 210 nmPeaks: 1. Oxalic acid 15 mg/L (ppm)2. Tartaric acid 1203. Formic acid 1804. Malic acid 1205. iso-Citric acid 1206. Lactic acid 1807. Acetic acid 1208. Citric acid 1209. Succinic acid 12010. Fumaric acid 711. cis-Aconitic acid **12. trans-Aconitic acid **** 7 ppm total for cis and trans isomers20011AU021 3 4 85 6 7291011 121013 485 6 7 9 11 122Injection 1Injection 10894 6 8MinutesColumn: Acclaim OA, 5 µmDimensions: 4 × 150 mmMobile Phase: 40 mM Na 2 SO 4 , pH(adjusted with methanesulfonic acid)Temperature: 30 °CFlow Rate: 1.0 mL/minInj. Volume: 5 µLDetection: UV, 210 nmPeaks: 1. Oxalic acid 15 mg/L (ppm)2. Tartaric acid 1203. Formic acid 1804. Malic acid 1205. iso-Citric acid 1206. Lactic acid 1807. Acetic acid 1208. Citric acid 1209. Succinic acid 12010. Fumaric acid 311. cis-Aconitic acid**12. trans-Aconitic acid*** *3 ppm total for cis andtrans isomers20012Gradient Separation of C1–C7 Aliphatic Organic Acids0.1800.100AU01234, 5Minutes6789 100 5 10 15 20Column: Acclaim OA, 5 µmDimensions: 4 × 250 mmMobile Phases: A: CH 3CNB: 2.5 mM methanesulfonic acidGradient: -10 0 1 15 20 min.2 2 2 60 60 %A98 98 98 40 40 %BTemperature: 30 °CFlow Rate: 1.0 mL/minInj. Volume: 30 µLDetection: UV, 210 nm with blank subtractionPeaks: 1. Formic acid 10 mmol/L2. Acetic acid 103. Propionic acid 104. Butyric acid 105. Isobutyric acid 106. Isovaleric acid 107. n-Valeric acid 108. Isocaproic acid 109. n-Caproic acid 1010. Heptanoic acid 10OA23611Ordering Information: Acclaim OAThe standard particle size is nominally 5 µm. Analytical columns are available in 4.0-mm diameters; standard lengths are 150 and 250 mm.Guard cartridges in 4.3 × 10 mm are recommended to protect your analytical columns. Other geometries in PEEK or stainless steel areavailable; inquire with your Dionex sales representative.Please order through your local Dionex office or distributor. Refer to the following part numbers.Acclaim OA Analytical Columns5 µm 4.0 × 150 mm ............................... 0629035 µm 4.0 × 250 mm ............................... 062902Acclaim OA Guard Columns5 µm 4.3 × 10 mm . ...............................062925Biocompatible cartridge holder for2.1 × 10 mm and 4.3 × 10 mm guards ..................059456Guard to analytical column coupler ...................059457Holder and coupler kit. ..............................059526These columns are designed for optimal performance using Dionex UltiMate 3000 and ICS-3000 chromatography instruments.www.dionex.com - 23 -
Page 2: Acclaim 120Acclaim 300Acclaim PA/PA
Page 5 and 6: IntroductionAcclaim General Purpose
Page 8 and 9: IntroductionINTRODUCTIONUnderstandi
Page 10 and 11: IntroductionINTRODUCTIONAcclaim Col
Page 12: Acclaim 120 C18120 C18Pa r t Tw o:
Page 24 and 25: Acclaim SurfactantChapter 7: Acclai
Page 26 and 27: Acclaim ExplosivesChapter 8: Acclai
Page 28 and 29: ApplicationsPa r t Th r e e: Applic
Page 30 and 31: Pharmaceutical ApplicationsAU10 1 2
Page 32 and 33: Pharmaceutical Applications00-24 mA
Page 34 and 35: Environmental ApplicationsEnvironme
Page 36 and 37: Environmental Applications0.5 Colum
Page 38 and 39: Food and Beverage ApplicationsAssay
Page 41 and 42: Food and Beverage ApplicationsSulfo
Page 43 and 44: Ch e m i c a l ApplicationsSurfacta
Page 45 and 46: Ch e m i c a l ApplicationsSurfacta
Page 47: Bioscience ApplicationsProteins and
Page 51 and 52: Lot Qualification TestsColumn Perfo
Page 53 and 54: Part Number IndexAppendix 3:Part Nu
Page 55 and 56: Analyte Indexcis-Lycopene..........

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