ACCLAIM oa - Cromlab

Food and Beverage ApplicationsAssay for Water-Soluble Vitamins inVitamin Tablets Using Acclaim PA015 6 7 8 Peaks: 1. Thiamine210 nm2. Ascorbic acid246 nm3. Pyridoxine265 nm285 nm4. NiacinamideColumn: Acclaim PA, 3 µm1000Dimensions: 4.6 × 150 mm2Mobile Phase: Phosphate pH 3.4,gradient 0–20% CH 3 CNDetection: UVD 340 at listed wavelengthsSample: One-A-Day ® brand "Active Formula"Sample Prep.: 1. Grind a 1500 mg tabletmAU2. Extract 150 mg with 100 mL of4pH 3.4 buffer3. Sonicate 10 min4. Filter5. Inject 20 µL3–2005. Pantothenic acid0 Minutes176. Folic acid7. Vitamin B 128. RiboflavinOne-A-Day is a registered trademark of Bayer Corp.19350Nitrofuran Antibiotic Residues in Animal Feed on Acclaim 120 C1835mAU012 30 2 4 6 8 10 12MinutesReference:R.J. McCracken, D.G. Kennedy;J. Chromatogr. A, 1997, 771, 349–354.420 mg/kg5 mg/kgControlColumn: Acclaim 120 C18, 5 µm,4.6 × 150 mmPump: Summit P680A DGP-6Mobile Phase: Acetonitrile:10 mM ammonium acetatepH 5.0, 20:80 v/v; isocraticFlow: 1.0 mL/minTemperature: 30 °CInjection: Summit ASI100, 20 µLDetector: Summit UVD340U diode array,365 nm, and spectra 200–500 nmSample Preparation:1. 3.0 g guinea pig feed in a 50 mL centrifuge tube2. Add 9 mL water and let stand 5 min.3. Add 21 mL methanol:acetonitrile 1:1and extract for 30 min.4. Pass through cleanup cartridge containing1.7g of neutral alumina; discard first 1.7 mL,retain next 3.5 mLPeaks: 1. Nitrofurazone2. Nitrofurantoin3. Furazolidone4. Furaltadone215582,500,000CountsSensitive Detection of Triazine Herbicides onAcclaim 120 C18 with MS Detection1 234502.9 18.6Minutes67Background Information: Seven herbicides at 1 ngon column. Seven simultaneous single-ionchromatograms optimized for positive identification.Column: Acclaim 120 C18, 5 µmDimensions: 2.1 × 100 mmMobile Phase: 35% Acetonitrile65% Ammonium acetate, pH 5Temperature: 30 °CFlow Rate: 0.25 mL/minInj. Volume: 10 µLDetection: MSQ MSESI: PositiveProbe: 400 °CNeedle: 2.5 kVCone Voltage: Set per ionPeaks m/z1. Simazine 2022. Atrazine 2163. Prometon 2264. Ametryn 2285. Propazine 2306. Prometryn 2427. Terbutryn 24220401300mAU0Fat-Soluble Vitamins and Carotenoids in a Vitamin Tablet12 3473 nm325 nm285 nm0 5 10 15 20MinutesSample Preparation:– Finely grind a weighed tablet.– Weigh an aliquot of about 1/10 tablet (150 mg)into a screw-cap vial.– Partition between 1.0 mL water and 5.0 mLethyl acetate with vigorous shaking.– Warm to 60 °C for 10 min.– Shake vigorously again for 0.5 min.– Centrifuge to separate phases.4567Column: Acclaim PA C16, 3 µm,4.6 × 150 mmPump: Summit P680A DGP-6Mobile Phase: (A) Water(B) 54:44:2 methanol:acetonitrile:isopropanolGradient: Times 0 8.0 8.1 25%A 5 5 0 0%B 95 95 100 100Flow: 1.25 mL/minTemperature: 30 °CInjection: Summit ASI-100, 15 µLDetector: Summit UVD 340U diode array,473, 325 and 285 nm,and spectra 200–595 nmPeaks: 1. trans-Retinol acetate2. trans-Lutein3. α-Tocopherol acetate4. trans-Lycopene5. cis-Lycopene6. trans-β-Carotene7. cis-β-Carotene21786APPLICATIONS- 38 -www.dionex.com