ACCLAIM oa - Cromlab

Food and Beverage ApplicationsParabens on Acclaim PA20.15Column:Dimensions:Acclaim PA2, 5 µm4.6 × 150 mm1Mobile Phase: CH 3 CN/H 2 O v/v 60/402Temperature: 30 °CFlow Rate: 1.0 mL/minAU3Inj. Volume: 2 µL4Detection: UV, 254 nmPeaks: ( 0.1 mg/mL each)1. Methylparaben2. Ethylparaben3. Propylparaben04. Butylparaben0 2 4 6 8 10Minutes20464450mAU01230 10 20 30Minutes*Obtained from Am. Soc. Brewing ChemistsHop Extract on Acclaim 120 C184Column: Acclaim 120 C18, 5µm, 4.6 × 150 mmPump: Summit P680A DGP-6Mobile Phase: Methanol, water, phosphoric acid;85:17:0.25 (v/v/v)Flow: 1.2 mL/minTemperature: TCC-100 thermostat, 35 °CInjection: ASI100 autosampler, 25 µLDetector: UVD-340U, UV 314 nm;spectra 200–400 nmStandard*: ICE-2 certified hop extract,986 µg/mL in acidified methanolPeaks: 1. Cohumulone 142 µg/mL2. Humulone + adhumulone 3443. Colupulone 1274. Lupulone + adlupulone 11821997Water-Soluble Vitamins Using Acclaim PA2Determination of Quinine in Tonic Water Using Acclaim PA0.3047 8AU235 6 9100 2 4 6 8 10 12 13MinutesColumn: Acclaim PA2, 5 µmDimensions: 4.6 150 mmMobile Phase: (A) CH 3 CN(B) 25 mM phosphate buffer, pH 3.31Gradient: Hold B for 5 minB to A/B (40/60) in 7 minthen to A/B (75/25) in 1 minHold A/B (75/25) for 4 minTemperature: 30 °CFlow Rate: 1.0 mL/minInj. Volume: 50 µLDetection: UV, 210 nmPeaks: 1. Thiamine 2.5 µg/mL2. Ascorbic acid 603. Pyridoxine 2.54. Niacinamide 205. Pantothenic acid 106. Vitamin B12 1.257. Folic acid 58. Riboflavin 59. Biotin 12.5202810.7AU0.0A. Tonic Water1B. Quinine standard,100 µg/mL20 51015MinutesReference: U.S. Pharmacopeia, 22nd Ed.,1990, 1209.34Column: Acclaim PA C16, 5 µm,4.6 × 150 mmPump: GP50 microboreMobile Phase: (A) 896 mL water,100 mL acetonitrile,1.40 mL methanesulfonic acid,0.80 mL acetic acid,2.00 mL diethylamine(B) 70:30 v:v acetonitrile:waterGradient: Time 0.0 10.5 10.6 15.0%A 100 100 50 50%B 0 0 50 50Flow: 1.0 mL/minTemperature: 30 °CInjection: 25 µLDetector: AD20, UV 235 nmSample Prep.: FilterPeaks: 1. Cinchonidine2. Quinine3. Dihydroquinine4. Benzoic acid21528Determination of Energy Drink Ingredients with Acclaim PA2600mAU0210nm246nm265nm285nm123 546780 4 8 12 16MinutesSample Prep:Peaks:Column: Acclaim PA2, 3 µm, 4.6 × 150 mmPump: Summit P680 DGP6Mobile Phases: (A) Acetonitrile(B) 30 mM phosphate buffer, pH 3.28(C) 30 mM phosphate buffer, pH 2.54Gradient: Times 0.0 14.0 16.0%A 0 40 40%B 100 0 0%C 0 60 60Flow: 1.0 mL/minTemperature: 30 °CInjection: ASI-100 autosampler, 5 µLDetector: UVD 340U, UV 210, 246, 265,285 nm; spectra 200–400 nmFilter1. Ascorbic acid2. Pyridoxine3. Niacinamide4. Pantothenic acid5. Caffeine6. Riboflavin7. Sorbic acid8. Benzoic acid21784300mAU0BA1 2 3 40 2 4 6 8 10 12MinutesSample Preparation:– Finely grind dried peppers– Take an aliquot of about 400 mg– Partition between 2.0 mL water and 6.0 mLethyl acetate with vigorous shaking for 2 min– Centrifuge to separate phases– Evaporate 1.0 mL of ethyl acetate phase andreconstitute in 0.33 mL ethanol5Sudan Dyes in PaprikaColumn: Acclaim 120 C18, 5 µm,4.6 × 150 mmPump: Summit P680 DGPMobile Phase: (A) Water(B) 1:1 tetrahydrofuran:methanolGradient: Times 0 7.0 7.1 13%A 20 8 0 0%B 80 92 100 100Flow: 1.25 mL/minTemperature: 30 °CInjection: Summit ASI-100, 15 µLDetector: Summit UVD340U diode array,500 nm, and spectra 200–595 nmSamples: (A) Control paprika without artificialcoloring(B) Paprika spiked with approx.25 mg/kg of each dyePeaks: 1. Sudan I2. Sudan II3. Sudan III4. Sudan IV5. Oil Red O (2 peaks)(Natural pigments 8-12 min.)21787APPLICATIONSwww.dionex.com - 39 -