ACCLAIM oa - Cromlab

Ch e m i c a l ApplicationsSurfactantsAUAnalysis of a Strongly Hydrophilic HydrotropeAcclaim SurfactantConventional C180 5 10 15MinutesDimensions: 4.6 × 150 mmMobile Phase: Acetonitrile/0.1 M NH 4 OAc,pH 5.4 v/v 30/70Temperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 5 µLDetection: UV, 225 nmSample: Sodium xylene sulfonate (1000 ppm)210744µS0Analysis of Ammonium Laureth SulfateUsing Conductivity Detection0 10 2030MinutesColumn: Acclaim Surfactant, 5 µmDimension: 4.6 × 150 mmMobile Phase: Acetonitrile/0.5 M H 3 BO 4and 20 mM NaOH v/v 55/45Temperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: Suppressed CD (AMMS III)Sample: Ammonium laureth sulfate21076Analysis of NEODOL 25-7Analysis of Quats Using LC-ESI-MSmVR—(OCH 2 CH 2 ) n OHR = C 12 to C 15n ~ 7NEODOL 25-7Column: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) H 2 OGradient: 40–65% A in 30 min,then hold at 65% A for 5 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: ELSDAbundanceC15C16C17C18Column: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: Acetonitrile/50 mM NH 4 OAc,pH 5.4 v/v 60/40Temperature: 25 °CFlow Rate: 1 mL/min (0.2 mL/min tomass spectrometer)Injection Vol.: 25 µLDetection: MS (ESI positive, probe 300 °C,cone 30 V, needle 2.5 kV, SIM 332.4,360.4, 374.4, 388.5, span 0.5 m/z)Peak: Benzyl dimethyl hydrogenatedtallow ammonium chloride (10 ppm)0 10 20 30 35Minutes210790 5 10 15Minutes22127APPLICATIONSmV121Analysis of Toilet Bowl Cleaner0.1 M NH 4 OAc0 5 10 15 20 25 30Minutes56 7 8430.02 M NH 4 OAcSeparation improved by changingmobile phase ionic strength54 6 738Column: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) CH 3 CN(B) NH 4 OAc, pH 5.4Gradient: 25% to 85% A in 25 min,hold for 5 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: ELS detectorSample: Toilet bowl cleaner(20× dilution)Peaks: 1. Chloride2. PEG3–7. Cationic surfactants8. Nonionic surfactant22797mVInorganic Anions, Hydrotropes, Cationic, Nonionic,Amphoteric, and Anionic Surfactants213Column: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) CH 3 CN,(B) 0.1 M NH 4 OAc, pH 5.4Gradient: 25% to 85% A in 25 min,then hold 85% A for 10 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 25 µLDetection: ELS detector45670 5 10 15 20 25 30 35Minutes89101112Peaks:1. Chloride2. Bromide3. Nitrate4. Xylene sulfonate5. Laurylpyridinium chloride6. Lauryldimethylbenzylammoniumchloride7. Triton X-1008. Cetyl betaine9. Decyl sulfate10. Dodecyl sulfate11. C 10 -LAS12. C 11 -LAS13. C 12 -LAS14. C 13 -LAS131422793- 44 -www.dionex.com