Analyst Software Reference Guide - AB Sciex

Software Reference GuideIDA MethodsAn IDA (Information Dependent Acquisition) method automatically runs experiments based onresults obtained from previous experiments. Use IDA criteria to optimize data acquisition settingswhile acquiring data to reduce the sample acquisition time in a single injection. With IDA, you canconserve both the sample amount required and working time.Create an IDA method with up to two survey scans and eight dependent scans in a singleexperiment. A survey scan is used in IDA to trigger additional experiments. You can use thefollowing scans as a survey scan:• Enhanced Multiply-Charged (EMC).• Enhanced Product Ion (EPI) (second survey scan).• Enhanced MS (EMS).• MRM.• Neutral Loss (NL).• Precursor Ion (Prec).• Q3 MS.You can use the following dependent scans:• EPI.• MS/MS.In an IDA experiment, the mass spectrometer actions are varied from scan to scan based on thedata acquired in a previous scan. The software analyzes data as it is being acquired and thendetermines the masses on which to perform dependent scans. You can set the criteria that willactivate an IDA experiment and the method parameters to be used.IDA experiments modify experiments and improve results based on the following user-definedcriteria:• Ion intensity and charge state.• Inclusion and exclusion lists.• Isotope pattern.• Dynamic exclusion.• Rate of change in ion intensity (See Dynamic Background Subtraction Algorithmon page 16).InfusionInfusion is the continuous flow of the sample at low flow rates into the ion source using a syringepump. During the infusion optimization process, the software can select precursor and productions and optimize for declustering potential, collision energy, and collision cell exit potential forboth. The voltages of these ion path parameters are gradually increased or decreased todetermine the maximum signal intensity for the precursor and product ions.Use infusion optimization to optimize compound-dependent parameters only at much lower flowrates than those used during LC/MS analysis. See also Flow Injection Analysis (FIA) on page 17.Release Date: August 2011 19