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GC COLUMNS | APPLICATION-SPECIFIC COLUMNSFoods, Flavors & FragrancesPolyunsaturated FAME AnalysisFAMEWAX Columns (fused silica)(polar phase; Crossbond® polyethylene glycol)• Application-specific <strong>columns</strong> for FAMEs, specially tested with a FAME mixture.• Temperature range: 20 °C to 250 °C.similar phaseOmegawaxThe elution order of polyunsaturated FAMEs on FAMEWAX <strong>columns</strong> is comparable tothat on other Carbowax® <strong>columns</strong>, but baseline resolution is achieved in significantlyless time.ID df temp. limits 30-Meter0.25mm 0.25µm 20 to 250°C 124970.32mm 0.25µm 20 to 250°C 124980.53mm 0.50µm 20 to 250°C 12499ChromatogramSearch ToolSearch by compound name, synonym,CAS # or keywordwww.restek.com/chromatogramsFAMEs (marine oil standard) on a FAMEWAX column.Column: FAMEWAX, 30m, 0.32mm ID, 0.25µm (cat.# 12498)Inj.: 1µLConc.:10,000µg/mL in isooctane(total FAMES; see breakdown in peak list)Oven temp.: 195–240°C at 5°C/min., 1 min. holdInj./det. temp.: 250°C/275°CCarrier gas: hydrogenFlow rate: 3mL/min. (constant flow)Split ratio: 100:1Columnengineered foroptimal resolution.Peak List Conc. (µg/mL)1. C14:0 6002. C14:1 1003. C16:0 1,6004. C16:1 5005. C18:0 8006. C18:1 (oleate) 1,3007. C18:1 (vaccenate) 4008. C18:2 2009. C18:3 20010. C20:0 10011. C20:1 90012. C20:2 10013. C20:4 30014. C20:3 10015. C20:5 1,00016. C22:0 10017. C22:1 30018. C24:0 10019. C22:6 1,20020. C24:1 100GC_FF00499Catch the BuzzSign up for Restek’s e-newsletter, The Buzzwww.restek.com/buzz70 www.restek.comWebsite : www.chromtech.net.au E-mail : info@chromtech.net.au TelNo : 03 9762 2034 . . . in AUSTRALIA Mar 2011

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