ANNUAL REPORT Kunming Institute of Botany, Chinese Academy ...

More documents

Recommendations

Info

Kunming Institute of Botany, Chinese Academy of SciencesAnnual ReportN-3-oxo-decanoyl-L-homoserine-lactone Activates Auxin-inducedAdventitious Root Formation via H 2 O 2 - and Nitricoxide-dependent Cyclic GMP Signalling in Mung BeanN-acyl homoserine-lactones (AHLs) are bacterialquorum-sensing signaling molecules that regulatepopulation density. Recent evidences demonstrate theirroles in plant defense responses and root development.H 2 O 2 , nitric oxide (NO) and cyclic GMP (cGMP) areessential messengers that participate in various plantphysiological processes, but how these messengersmodulate the plant response to AHL signals remainspoorly understood.Researchers at KIB show that the N-3-oxodecanoyl-homoserine-lactone(3-O-C10-HL), in contrastto its analogue with an unsubstituted branch chain atthe C3 position, efficiently stimulated the formation ofadventitious roots and the expression of auxin-responsegenes in explants of mung bean seedlings. This responsewas mimicked by the exogenous application of auxin,H 2 O 2 , NO or cGMP homologues, but suppressed bytreatment with scavengers or inhibitors of H 2 O 2 , NOor cGMP metabolism. The 3-O-C10-HL treatmentenhanced auxin basipetal transport; this effect couldbe reversed by treatment with H 2 O 2 or NO scavengersbut not by inhibitors of cGMP synthesis. Inhibiting3-O-C10-HL-induced H 2 O 2 or NO accumulationimpaired auxin- or 3-O-C10-HL-induced cGMPsynthesis, however, blocking cGMP synthesis did notaffect auxin- or 3-O-C10-HL-induced H 2 O 2 or NOgeneration. Additionally, cGMP partially rescued theinhibitory effect of H 2 O 2 or NO scavengers on 3-O-C10-HL-induced adventitious root development and auxinresponsegene expression. These results suggest that3-O-C10-HL, unlike its analogue with unmodifiedbranch chain at the C3 position; can accelerate auxindependentadventitious root formation possibly viaH 2 O 2 - and NO- dependent cGMP signaling in mungbean seedlings.The study has been supported by the Hundredsof Talents Program of CAS and the National NaturalScience Foundation of China, and has been published inPlant Physiology (2012, 158:725-736).The effects of different inhibitors or scavengersand donors on 3-O-C10-HL-induced accumulationof H 2 O 2 and NO in mung bean explants. —— HIGHLIGHTS OF ACHIEVEMENTS ——
Kunming Institute of Botany, Chinese Academy of SciencesAnnual ReportAM Fungi Suppress Haustorium Initiationin a Root Hemiparasitic PlantAs two ubiquitous and important components interrestrial ecosystems, parasitic plants and arbuscularmycorrhizal (AM) fungi have been extensivelystudied in the past, but in most cases separately.Recent findings suggest that plant parasitism andAM associations have some interesting parallels andmay be modulated by similar mechanisms. Despitethe small but increasing number of efforts to test theinfluence of host AM status on growth performanceof parasitic plants, no investigations have tested thedirect interactions between parasitic plants and AMfungi, partially due to the fact that most parasiticplants do NOT form mycorrhizal associations.Using a facultative root hemiparasite(Pedicularis tricolor Hand.-Mazz.) with a potentialto form AM associations, researchers from KIB (Dr.LI Ai-Rong) and University of Adelaide show thatinoculation with some AM fungi strongly suppressesthe initiation of haustoria, the parasite-specificstructures exclusively responsible for host attachmentand nutrient transfer from a host to a parasitic plant.The significant role of AM fungi in haustoriuminitiation of parasitic plants is demonstrated for thefirst time.The research was supported by the NaturalScience Foundation of China (grant No. 30970288),the Natural Science Foundation of Yunnan Province(grant No. 2009CD114) and the Overseas TrainingProgram of CAS for LI Ai-Rong. The results havebeen reported in Annals of Botany (2012, 109: 1075-1080).Effects of arbuscular mycorrhizal (AM) fungal inoculation on haustorium formation in Pedicularistricolor. Haustoria are visible as swellings on roots, and some examples are shown with tailedarrows. (A) Presence of the host plant (barley) but without AM inoculation. The inset shows twohaustoria (H) with a xylem bridge (XB) connecting a root of P. tricolor (PR) to a root of barley (BR).(B) Presence of the host plant (barley) with inoculation of Glomus intraradices. Note the lowerdensity of haustoria compared with (A). The inset shows a haustorium with an XB connecting PRand BR. Colonization of barley by the AM fungus is also shown (AM).—— HIGHLIGHTS OF ACHIEVEMENTS ——
Page 2 and 3: Cover photos:51 3 62 4 71. Trogia v
Page 4: ACHIEVEMENTS AND AWARDS ...........
Page 7: Kunming Institute of Botany, Chines
Page 10 and 11: Kunming Institute of Botany, Chines
Page 35 and 36: SUMMARY OF ACCOUNTSIncome and Expen
Page 66 and 67:
Kunming Institute of Botany, Chines
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 132:
Add: 132# Lanhei Road, Heilongtan,K
show all

ANNUAL REPORT Kunming Institute of Botany, Chinese Academy ...

Create successful ePaper yourself

Delete template?

Save as template?