General Catalogue Chemistry - BECO Internacional LTDA.

chromatography
HPLC
in Education and Training
HPLC experiment setup using the CPS system
High performance liquid chromatography
(HPLC) is one of the latest chromatographic
separation processes, which, due to its versatility,
is currently enjoying increasing popularity.
HPLC can be used in a variety of areas and analytical
problems in biology, environment protection
and chemistry.
Equipment list
HPLC-chromatography
Quantity Description Cat. No.
1 CPS HPLC pump 665 485
1 CPS-HPLC column support 665 481
1 Separating column Sl RP-18 665 4791
1 CPS HPLC reservoir 665 487
1 HPLC basic kit 665 4801
1 Set of hoses and septa for HPLC 665 488
1 CPS Flow-through-photometer for HPLC 665 486
1 CPS mains distributor 665 497
1 Connecting lead, Ø 2.5 mm, yellow/green, cm long 501 41
1 Reagent and shipping bottle: 500 ml 661 203
1 Set of hoses and septa 665 488
1 Sensor-CASSY 524 010
1 CASSY Lab 524 200
1 Panel frame, C50, two-level 666 425
additionnally required:
1 PC with Windows 95 or 98 or NT
48 LEYBOLD DIDACTIC · GENERAL CATALOGUE CHEMISTRY
Natural products, organic polymers and biopolymers,
pharmaceuticals, dyes, antioxidants, insecticides,
protein mixtures, nucleic acid fragments,
fatty components, pheromones or plant
pigments, are just some of the substances suitable
for analysis using HPLC.
HPLC is capable of far higher separation than
traditional column chromatography. The higher
pressures substantially reduce the time required
for analytical separation. In routine measurements,
separation requires only a few minutes,
no longer hours or days.
A further result of the higher operating pressures
is, that extremely fine-grained carrier materials
can be used. This increases the exchange
between the stationary and mobile phases (the
number of “theoretical plates“ increases
substantially).
An inexpensive low-pressure system (max. 10
bar) has been designed especially for educational
use. HPLC is recommended for use in advanced
secondary science courses, as well as in occupational
training.
The eluent (the mobile phase) is transported
from the liquid reservoir through the pump and
the separating column (containing the stationary
phase). The sample to be analyzed is transported
to the column via the column head. The end of
the column is connected to a detector, and this
signal is registered by recorder. Alternatively,
using a computer makes for more efficient
recording and evaluation of measurements.
The LEYBOLD DIDACTIC HPLC system is
based on a new concept for fast column chromatography
using pressures of up to 6 bar. It comprises
individual functional modules which,
depending on necessity, can easily be assembled
to create the entire HPLC system. All components
are mounted on training panels. A wide
variety of separating columns is available. An
aeration pump with highly constant pumping
capacity serves as the pump for this system. A
UV/VIS-photometer with flow-through cell, or
even more effective, the CPS flow-through photometer
can be used as the detector. Control and
regulation of the entire system is carried out
using the CASSY interface device.
Comprehensive instructions enable easy installation
of the system. Experiment instructions for
HPLC in education and training can be found in
the manual “Biochemistry II“ (> 668 102).