Effects of isochaborat testosterone on the kidney rats

Materials and Methods
(Sustanon ®250) was being administered weekly for two months based
on the body weight.
1.5.Specimens collection
At the end date of experiment (the two months) of treatment, all rats were
scarified after giving deep anesthesia by with phenobarbital (50 mg/kg body
weight).
The rat abdomen was opened, the kidney was dissected out, cut into 2-3
mm segments.
The kidneys were excised and processed for light and electron
microscopic examination.
The changes in the kidney associated with the dose of sustanon of each
group of rats were studied and examined using light and electron microscopic
study.
Light microscopic studies to assesed the histological structure of the
cortical and medullary regions of the kidney.
Electron microscopic studies to assesed epithelial cells of renal tubeules
and interstitial changes.
Used (Sustanon ®250) (250mg/ml) and the volume used was 1ml in
different concentrations and sesame oil.
Slides were examined under the light and electron microscopes in low
and high magnifications.
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