Effects of isochaborat testosterone on the kidney rats

Materials and Methods
1.1.6.Apply the Eosin Counterstain
The section is now stained with an aqueous or alcoholic solution of eosin
(depending on personal preference). These colors many nonnuclear elements in
different shades of pink.
1.1.7.Rinse, Dehydrate, Clear and Mount (Apply Cover
Glass)
Following the eosin stain, the slide is passed through several changes of
alcohol to remove all traces of water, and then rinsed in several baths of xylene
which “clears” the tissue and renders it completely transparent. A thin layer of
polystyrene mountant is applied, followed by a glass coverslip. If the stain and
all the subsequent steps have been properly performed, the slide will reveal all
the important microscopic components and be stable for many years.
In short: Tissue Processing used for light microscopic examination was
(Paraffin Method). Specimens were prepared by gross fixed then perfused in
10% neutral buffered formaline for 48-72 hours, followed by a dehydration
using a series of graded ethanol in ascending concentrations (50%, 70%, 95%,
and 100%), immersed in xylene for clearing, infiltrated in paraffin wax, and
finally embedded in paraffin wax. 4-6 micrometer thick paraffin sections were
obtained by using Rotary Microtome to inspect for histoarchitectural changes at
(200x and 400x ) magnification (Bancroft et al., 2013).
2-Electron microscopic examination:
According to the method described by Layton and Suvarna for the
electron microscopic analysis, cortex and medulla of kidneys tissues was
processed for electron microscope (Bancroft et al., 2013).
In the following sections, the basic steps to prepared slides are outlined.
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