Effects of isochaborat testosterone on the kidney rats

Materials and Methods
Species
Body
Weight (kg)
Body
Surface Area
(m2)
Conversion
Factor
Adult Human 60 1.6 1.00
Dog 10 0.5 1.85
Rabbit 1.8 0.15 3.08
Rat 0.15 0.025 6.17
(Table 1) Data obtained from Food and Drug Administration draft guidelines (FDA).
2. Methods:
Specimens from the kidnyes of all rats were excised and prepared for
histological examination by the light and electron microscope.
Histological examination of the kidneys tissues
1.1.light microscopic examination:
After sacrificing the rats, their kidneys were dissected and cut into small
pieces (0.5 cm).
Staining was used:
Hematoxylin and Eosin (Hand E) stain (Feldman and Wolfe 2014).
In the following sections, the basic steps in performing an Hand E stain
are outlined.
1.1.1.Remove the Wax
Following the preparation of a paraffin section, all the elements are
infiltrated with and surrounded by paraffin wax which is hydrophobic and
impervious to aqueous reagents. The majority of cell and tissue components has
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