Effects of isochaborat testosterone on the kidney rats

Materials and Methods
• Ethanol 90% 10 minutes
• Ethanol 100% 10 minutes
• Ethanol 100% 10 minutes
• Ethanol 100% 10 minutes
The specimen then cleared in three changes of propylene oxide 15
minute each. The specimens then impregnated in two successive mixtures of'
propylene oxide and epon {(1:1) for one hour, then, (2:3) for six hours} and
finally in epon alone for 12 hours at room temperature.
Tissue specimens were embedded with epon in a flat silicone rubber
molds and then polymerized at 60 ºc for 48 hours after appropriate orientation
using fine wooden stick.
2.8. Sectioning:
Rectangular flat blocks containing the specimens, were removed from
the moulds and trimmed by clean razor.
Both semi-thin (1-µm) and ultra-thin (~80nm) sections from the kidney
were cut using ultra microtome (Reichert-Jung, Licea Germany) using glass
knifes for semi-thin sectioning and diamond knife (Reichert-Jung, Licea
Germany) for ultra-thin sectioning.
The glass knifes were prepared by cutting triangular pieces with sharp
apical edge from 5 square inch glass plates of 6 mm thickness using glass
cutting device (Reichert-Jung, Licea Germany).
2.9. Staining:
The semi-thin sections were mounted on glass slide and stained with
Toluidine blue. Thin sections were mounted on cupper microgrids of 200
meshes (Reichert-Jung, Licea Germany) and stained with 0.2% lead citrate and
aqueous solution of uranyl acetate.
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