The Staphylococcus aureus secretome - TI Pharma

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Chapter 6 Table 1. Bacterial strains and plasmids used Plasmids Properties Reference TOPO pCR®-Blunt II-TOPO® vector; Km R Invitrogen Life technologies “srtA”-TOPO TOPO plasmid containing the flanking regions of S. aureus srtA, Kan R This work srtC-pUC18 pUC18 plasmid with the S. epidermidis srtC gene, Amp R This work “srtA”SE-TOPO TOPO plasmid containing the flanking regions of S. epidermidis srtA, Kan R This work pCN51 E. coli - S. aureus shuttle vector that contains a cadmiuminducible promoter (Charpentier et al., 2004) pMAD E. coli - S. aureus shuttle vector that is temperaturesensitive in S. aureus and contains the bgaB gene, Ery R , (Arnaud et al., 2004) Amp R pUC18 Amp R , ColE1, F80dLacZ, lac promoter (Norrander et al., 1983) Sa-srtA-pCN51 pCN51 with S. aureus srtA gene, Amp R ; Ery R This work Se-srtA-pCN51 pCN51 with S. epidermidis srtA gene, Amp R ; Ery R This work Se-srtC-pCN51 pCN51 with S. epidermidis ATCC12228 srtC gene, Amp R ; Ery R This work Bs-yhcS-pCN51 pCN51 with B. subtilis yhcS gene, Amp R ; Ery R This work Strains E. coli Genotype Reference DH5α supE44; hsdR17; recA1; gyrA96; thi-1; relA1 (Hanahan, 1983) TOP10 Cloning host for TOPO vector; F - mcrA ∆(mrr-hsdRMSmcrBC) Φ80lacZ∆M15 ∆lacX74 recA1 araD139 ∆(araleu)7697 galU galK rpsL (Str R S. aureus RN4220 ) endA1 nupG Invitrogen Life technologies Parental strain Restriction-deficient derivative of NCTC 8325, cured of all known prophages (Kreiswirth et al., 1983) ∆srtA srtA This work ∆srtA ∆spa srtA spa; replacement of spa by kanamycin resistance marker; Kan R This work ∆srtA-comp-Sa ∆srtA strain complemented with S. aureus srtA through Sa-srtA-pCN51 This work ∆srtA-comp-Se ∆srtA strain complemented with S. epidermidis srtA through Se-srtA-pCN51 This work ∆yhcS-comp-Bs ∆srtA strain complemented with B. subtilis yhcS through Bs-yhcS-pCN51 This work ∆srtA-comp-srtC S. aureus SH1000 ∆srtA strain complemented with S. epidermidis srtC throught Se-srtC-pCN51 This work Parental strain Functional rsbU+ derivative of 8325-4 rsbU+, agr+ (Horsburgh et al., 2002) ∆srtA rsbU+, agr+, srtA This work ∆srtA ∆spa rsbU+, agr+, srtA spa; replacement of spa by kanamycin resistance marker; Kan R This work ∆srtA-comp-Sa ∆srtA complemented with S. aureus srtA through SasrtA-pCN51 This work ∆srtA-comp-Se ∆srtA complemented with S. epidermidis srtA through Se-srtA-pCN51 This work ∆yhcS-comp-Bs ∆srtA complemented with B. subtilis yhcS through BsyhcS-pCN51 This work ∆srtA-comp-srtC ∆srtA complemented with S. epidermidis srtC through Se-srtC-pCN51 This work sasG-pMUTIN4 Overexpression of SasG due to integrated pMUTIN4 plasmid; Ery R (Corrigan et al., 2007) sasG-pMUTIN4 ∆srtA ∆srtA; overexpression of SasG due to integrated pMUTIN4 plasmid; Ery R 116 This Work
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The Staphylococcus aureus secretome
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RIJKSUNIVERSITEIT GRONINGEN The Sta
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Paranimfen: Thijs R.H.M. Kouwen Mon
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Table of contents Chapter 1. Genera
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Chapter 1 Introduction and scope of
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Introduction and scope of this thes
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Introduction and scope of this thes
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Chapter 2 Mapping the pathways to s
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Mapping the pathways to staphylococ
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“Music is the medicine of the min
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Chapter 3 Summary Sequencing of at
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Chapter 3 transcription analyses, S
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Chapter 3 Lina et al., 2003). The a
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Chapter 3 (Supplemental tables IVb)
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Chapter 3 Figure 1. Characterizatio
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Chapter 3 Figure 3. Relative amount
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Chapter 3 strain of a patient who s
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“Music is my religion” -Johnny
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Chapter 4 Summary Staphylococcus au
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Chapter 4 secretion pathways have b
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Chapter 4 has been shown that S. au
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Chapter 4 Table 2 Plasmids and bact
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Chapter 4 Table 3 Primers used in t
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Chapter 4 containing X-gal and incu
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Chapter 4 assay, 25 L4-stage nemato
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Chapter 4 D O 540 18 16 14 12 10 8
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Chapter 4 These findings suggested
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Chapter 4 precursors by lgt mutant
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Page 186: Chapter 5 Synthetic effects of secG
Page 190: Synthetic effects of secG and secY2
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Page 222: Chapter 6 Partially overlapping sub
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Page 240: Chapter 6 Biofilm formation Biofilm
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Page 248: Chapter 6 Complementation analysis
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Page 264: Chapter 7 Summary Bacillus subtilis
Page 268: Chapter 7 Materials and Methods Bac
Page 272: Chapter 7 Mutants of S. aureus were
Page 276: Chapter 7 we deployed this assay to
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Chapter 7 since the growth of B. su
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Chapter 7 Discussion In the present
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Chapter 7 Acknowledgements We thank
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“Although one can get very clever
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Chapter 8 Summary The now finished
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Chapter 8 Materials and methods Str
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Chapter 8 The extracellular proteom
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Chapter 8 There were also some cell
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Chapter 8 secreted only at a low le
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Chapter 8 by glucose as well as by
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“I will choose free will” -Neil
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Chapter 9 General summary and discu
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Chapter 9 Systematic analysis of tr
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Chapter 9 sequence of B. lichenifor
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“Good music is good music and eve
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Chapter 10 Adhikari, R.P., and Novi
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Chapter 10 Burts, M.L., Dent, A.C.,
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Chapter 10 Fedtke, I., Götz, F., a
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Chapter 10 Huard, C., Miranda, G.,
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Chapter 10 Marraffini, L.A., Ton-Th
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Chapter 10 Otto, M. (2008) Targeted
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Chapter 10 Siegers, K., Heinzmann,
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Chapter 10 Vrontou, E., and Economo
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“I only got seventh-grade educati
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Chapter 11 Nederlandse samenvatting
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Chapter 11 Inleiding - Hoofdstuk 1
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Chapter 11 verwijderen van deze com
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Chapter 11 gemaakt, maar er was nog
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Appendix I Dankwoord En nu je einde
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Appendix I regelmatig schoonmaken h
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Appendix II Publication list Public
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Appendix III: Supplemental tables t
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Appendix III Supplemental Table III
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Appendix IV Supplemental Table IVa
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Appendix IV Supplemental Table IVb
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Appendix IV Supplemental Table IVc
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Appendix V Supplemental Table Va ID
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Appendix V Supplemental Table Vb Su
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Appendix V Supplemental Table Vb Pr
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Appendix V Supplemental Table Vb Pr
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Appendix V Supplemental Table Vc Pr
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