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The Staphylococcus aureus secretome - TI Pharma

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Appendix V Supplemental Table Vb<br />

Protein Function pI Mr ID number md signal peptide sequence P G N LB ex LB st<br />

Phage-related functions<br />

XkdG PBSX prophage 5.14 34.3 BLi01331 x x x<br />

XkdK PBSX prophage 4.54 48.5 BLi01337 x x x x<br />

XkdM PBSX prophage 4.70 16.4 BLi01338 x x x x<br />

Unknown<br />

YbdN 5.35 30.7 BLi00302 MKKSLFLFVFSLFLMAIPAFS AS x x x x<br />

YdaJ 5.04 41.3 BLi04232 MKAPVRYIWIGMILCFLSVSLAVGCIKA ED x<br />

YkwD 10.16 26.6 BLi01607 MKKAFLLSAAAAATLFTFSGVQHA DA x<br />

YpjP 5.38 23.4 BLi02321 MKMWMRKALVALF<strong>TI</strong>ATFGLVSPPAALMA DK x<br />

YqgU 5.24 41.4 BLi02656 MAGLRVSLLIIAALMAVAAAG CTP x x<br />

YusA 6.45 30.5 BLi03455 MKKGLLTALFIIFAGVLAA CGS x x x x<br />

YusW 4.46 16.6 BLi03476 MNQFRMAVIALVLILMTG CGS x x<br />

YwoF 4.57 51.5 BLi02095 MRKWYFILSACILVSVIIAFA YD x x x x<br />

BLi00654 6.10 25.9 BLi00654 MKKAMAILGFLVLTASLLFIINKGTSQINA SQ x x x<br />

Unknown<br />

BLi00784 9.06 60.6 BLi00784 MKIQKRVQALLATSAMFAGLMLSDAVYA AE x<br />

BLi01431 4.56 18.6 BLi01431 MKKFLVLFLSFGLALALAA CSS x x x<br />

BLi02210 4.63 34.9 BLi02210 MLMSAFVLVLAACQQADPKGSYA AA x x x<br />

BLi02558 5.12 34.9 BLi02558 MKKWKSLSWMVLLLTLVMGLAA CGS x x x x<br />

BLi03010 9.50 34.9 BLi03010 MSKIKWIITL<strong>TI</strong>CTALAFSLFIFFNKA NF x x<br />

BLi03178 5.89 22.9 BLi03178 MKKLLKWTSALGLALSLALLAA CGN x<br />

BLi03260 4.68 37.4 BLi03260 2 MKKRLMSLLVCILVLVPAAGAFA AP x<br />

BLi03670 4.06 17.6 BLi03670 MKKLLFMVILSVLTLVFGSSSVSFA KD x<br />

BLi04124 6.80 48.5 BLi04124 MKRIYIFLLCFAVLLPVGGKTAQA KE x x x<br />

BLi04294 9.46 37.5 BLi04294 MVFKKPKVFIAAVILALSSFAGTAAYA QS x x x x x<br />

BLi04308 10.4 14.1 BLi04308 MKNHLYEKKKRKPLTR<strong>TI</strong>KATLAVLTMSIALV<br />

GGATVPSFA WV<br />

x x<br />

Signal peptides were predicted as described in the section on signal peptide prediction. <strong>The</strong> signal peptidase cleavage site is indicated with a gap in the amino acid sequence and the twinarginine<br />

motif in the signal peptides is highlighted in bold letters.<br />

md: number of additional predicted membrane spanning domains, P: phosphate starvation, G: glucose starvation, N: nitrogen starvation, LB ex : cells grown in LB, exponential growth phase,<br />

LB st : cells grown in LB, stationary growth phase.<br />

a this protein was found only in the extracellular proteome after 72 h cultivation (gel not shown).<br />

254

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