The Staphylococcus aureus secretome - TI Pharma

Mapping the pathways to staphylococcal pathogenesis by comparative secretomics
Table 3. Secretion machinery of S. aureus, S. epidermidis and B. subtilis
Sec-pathway S. aureus S. epidermidis B. subtilis
Chaperone Ffh + + +
FtsY + + +
FlhF - - +
CsaA - - +
Translocation motor SecA1
+
+
+
SecA2
+
+
-
Translocation channel SecY1
+
+
+
SecY2
+
+
-
SecE + + +
SecG + + +
SecDF + + +
YajC (YrbF) + + +
Lipid modification Lgt + + +
Sec-pathway S. aureus S. epidermidis B. subtilis
Signal peptidase SpsA (inactive) + + -
SpsB (SipSTUV) + + a +
SipW (ER-type) - - +
LspA + + b +
Folding catalyst PrsA + c + +
BdbC - - +
DsbA (BdbD) + + +
Cell wall anchoring SrtA + + -
SrtB + - -
SrtC - + d -
SrtD - - +
Tat-pathway
Translocase TatA
TatC
Pseudopilin pathway
Bacteriocins
Holins
Ess
25
+
+
ComGA + + +
ComGB + + +
ComC + + +
Bacteriocin-specific
ABC-transporters
-
-
? ? +
CidA (holin) + + +
LrgA (anitholin) + + +
EsaA + - +
EsaB + - +
EsaC + e - -
EssA + - -
EssB + - +
EssC + - +
Based on BLAST searches with the corresponding proteins of B. subtilis in the finished genome database
(http://www.ncbi.nlm.nih.gov/sutils/genom_table.cgi).
a
Two potentially active type I SPases are present in this strain and share homology to B. subtilis SipS and SipU
b
Two LspA proteins present in this strain
c
This protein is truncated at the C-terminus in the S. aureus JH9 strain
d
The genome of S. epidermidis RP62A only contains a srtA gene, whereas the genome of S. epidermidis
ATCC12228 also contains a srtC gene
e
This protein is missing in the S. aureus MRSA252 strain
The staphylococcal protein export pathways that have been characterized experimentally or
that can be deduced from sequenced genomes are schematically shown in Figure 4 and will be
discussed in the following sections. Since these pathways are likely to be used for the export
+
+