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Figure 11. Chromatograms of standard tenofovir (2000 ng/ml).<br />
Figure 12. Chromatograms of standard emtricitabine (2000 ng/ml).<br />
(2005), our SPE method showed relatively low recovery.<br />
However, in the method established by us, after once<br />
extracted, the column could be washed and activated by<br />
Tan et al. 1899<br />
methanol and water for three times. So, each column for<br />
SPE can be repeatedly used at least 3 times by a<br />
repeated validation experiment (% R.S.D < 15%) in our