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Chen et al. 1953
Figure 4. Concentration of TNF-α, IL-1 and IL-6 of plasma in four groups. (A) IL-1; (B); IL-6; (C) TNF-α. Data presented means ±SD. **P < 0.01 versus sham group; #P < 0.05, ## P 0.05) were lower than group TH (48.7 ± 7.9
U/g). MDA content in group TH + PHC was lower
than group TH + ANI, but no significant difference
was found in these two groups (P > 0.05). On the
other hand, MPO activity in group TH + PHC was
lower than group TH + ANI (P < 0.05).
The concentrations of,IL-1, IL-6, TNF-α in
plasma
Figure 4 shows the data of IL-1 (Figure 4A), IL-6
(Figure 4B) and TNF-α (Figure 4C) concentration
in plasma. Compared with sham group (IL-1: 9.8 ±
3.1 pg/ml; IL-6: 16.8 ± 7.4 pg/ml; TNF-α: 6.4 ± 3.7
pg/ml), the concentrations of IL-1, IL-6, and TNF-α
(P < 0.01) in groups suffered two-hits were significantly
elevated. Group TH + PHC and group TH +
ANI notably lessened the concentrations of IL-1,
IL-6, and TNF-α in plasma when compared with
group TH (IL-1:781.3 ± 145.1 pg/ml; IL-6:723.9 ±
163.9 pg/ml; THF-α: 736.2 ± 177.1 pg/ml; P <
0.05). The concentrations of IL-1 (453.1 ± 193.7
pg/ml), IL-6 (481.2 ± 176.9 pg/ml) and TNF-α
(494.2 ± 166.8pg/ml) in plasma in group TH + ANI
were notably higher than group TH + PHC (IL-1:
211.3 ± 67.8 pg/ml; IL-6: 273.7 ± 90.1 pg/ml; THFα:
262.4 ± 162.1 pg/ml, P < 0.05).
Histological examination
The structure of the renal glomeruli and tubuli,
proximal and distal convoluted tubes were seen
clearly in the sham group (Figure 5A). Severe
tubular dilatation and obstruction, glomerulus
sclerosis and necrosis, and the development of
protein casts were presented in the kidney of the
rats in group TH (Figure 5B). Patchy glomeruli
and interstitial hemorrhage, tubular epithelium
swelling, were found in group TH + PHC (Figure
5C) and group TH + ANI (Figure 5D), the damage
severity of renal tissues in these two groups (TH +
PHC: 9.8 ± 1.2; TH + ANI:18.1 ± 1.2) were
significantly lower than group TH (27.7 ± 1.2, P <
0.05). The mean grade scored of the rats treated
with PHC was lower than the rats treated with ANI
(P < 0.05).
Immunohistochemical detection of ICAM-1
Figure 6 indicates the representative
immunohisto-chemical detections of ICAM-1