Download Profil Badan Litbang Kesehatan 2012

MTB bacteria from the entirestudy sample wereinoculated in liquidmedium BACTEC MGIT 960 and solidmedia Lowenstein Jensen (LJ).3. Resistance PatternsAgainst all strains of MTBthatwere successfully grown were thentested the for sensitivity to the Anti TuberculosisDrugs (OAT) line I (Streptomycin,INH, Rifampicin and ethambutol).OAT sensitivity test results on anumber line I 325 of 338 total cultureculture culture samples (96.1%) indicatedthat some types of samples 202 MTBis still sensitive to OAT line I (62.3%),while the number of 108 samples (30.1%) showed resistant to INH and notOAT Rifampicin and 15 samples (4.6%)showed resistance to INH or Rifampicinonce commonly called type MTB-Multi Drug Resistance (MDR). Kappatest as an external quality control is implementedin the Central Health Laboratory(BBLK) Surabaya, which is theNational Reference Laboratory for testingresistance proportional method inIndonesia. Kappa test value is equal to89.02%, which means the test has metthe requirementsfor good resistance.4. Primary development of moleculardiagnostic methods of TBLoop-mediated isothermal a m -plification (LAMP)Molecular diagnostics for rapididentification of pulmonary TB casesdeveloped byR & D Health is a methodof loop-mediated isothermal amplification(LAMP). The reason is because thismethod uses simple instruments such asthe water bath (water bath) or heatingblock (heating plate) for identificationof MTB DNA in the sample.The results are new LAMP primer,based on the characterization ofMTBbacteria from samples that havebeen collected (originating from 16 provincialcapitals in Indonesia). Primaryhas filed his patent to the DirectorateGeneral of Intellectual Property Rights.Rapid detection of TB test resultson samples of TB patients in IndonesiaLAMP method using the LAMP primerdesign of Research and Development(Lisdawati, et al.) Compared with theprimary commercial LAMP showeda better sensitivity, which is capableof identifying up to 100 fg / mL MtbH37Rv DNA as the primary sensitivityof the LAMP commercial (Iwamoto etal) is only able to identify up to 1 pg /mL Mtb H37Rv DNA. Thisdata indicatethat the LAMP primer design Indonesiahas good prospects to be tested forfurther validation and validation testshave been carried out in 2011 to obtainprimary data sensitivity and specificity.Validation test process still continuestoday.The overall test of the primarydevelopment of molecular diagnosticmethods of TB Loop-mediated isothermalamplification (LAMP) also produceStandard Operating Procedures (SOPs)LAMP-TB test developed by the Researchand Development and who hasfiled copyright to the Directorate Generalof Intellectual Property Rights.Benefits :1. Mapping or phylogenetic dataMYBbacteria circulating in Indonesiawould be very useful for survillenceofTB because of factorsrelated to the preparation of drugdevelopment and diagnostic modelsappropriate to the character of existingbacteria.2. MTB culture bacterial strains originatingfrom 16 provinces in Indonesiacapital can be utilized for TBvaccine seed Indonesia.3. Map of the initial pattern of resistancein 16 provincial capitals in Indonesiacan be used as input for TBsurveillance governance includesdrugs and methods of promotion /preventive in terms of PHBs (Cleanand Healthy Behavior) for areaswith high MDR data.4. National independence is a step forthe development of rapid diagnosticfor TB cases.Profil Balitbangkes23