Products at a Glance 2024

Cell Culture Tools •

Cell Culture Tools • Cryo- and Cold-Management • Cell Culture supplements • Extracellular Matrices • Cell Culture Reagents • Transfection Tools • Reprogramming Tools • Safety Tools www.pelobiotech.com Cryo- & Cold-Management Cold- and cryo-preservation are critical steps while working with tissues and cells. Noteworthy to explain the importance of optimal, reproducible, and reliable cell work in clinical trials, cell therapies and regenerative medicine applications for humans and animals. It is obvious that basic research, drug development and compound testing will need the highest possible quality of cellular systems, too. Preservation is a critical, and often underappreciated, step in all research and clinical projects where tissues and cells will be used. It is best to optimize this process as early as possible to ensure reliable research, predictive compound testing, effective cellular therapies and highest possible cell/tissue quality. Cells can be isolated via cell separation (magnetic or non-magnetic procedures) or by tissue dissociation enzymes (collagenase & protease). In both cases the quality of cell source will be a critical factor. Cold preservation using special media which will help to keep the quality of tissues as high as possible. In many procedures cold- as well as cryo-preservation will be done. Cold-Preservation-Hypothermic Storage Storage of tissues and cells at low temperatures (4-10°C) for short periods (hours up to days) is defined as hypothermic storage. In the field of regenerative medicine cold preservation is an extension of principles and technologies used for whole-organ storage. Different media are available in the market for this application which were developed in many cases long before the explosion in cell therapy and tissue engineering. New media like HypoThermosol-FRS (BioLife) or Thermo-Gold (Revive) take the effects of cold shock damage (via stress pathways which are involved in necrosis and apoptosis) into account. Normally, cell or tissue function will be restored after 24 hours in culture at 37°C. Nevertheless, it is important to observe the post hypothermic storage viability for a week. Cells and tissues showing a high viability after warming can show a dramatic decrease after a few days. These effects depend on tissue or cell type. 32

www.pelobiotech.com Cold-Preservation Products: HypoThermosol ® FRS Thermo-Gold TM HypoThermosol FRS (HTS-FRS) is an optimized hypothermic (2-8°C) preservation media that enables improved and extended preservation of cells, tissues and organs. HTS-FRS is uniquely formulated to address the molecular-biological response of cells during the hypothermic preservation process. HTS-FRS includes key ions at concentrations that balance the intracellular state at hypothermic temperatures. Additional components include pH buffers, energy substrates, free radical scavengers, and osmotic/oncotic stabilizers. Thermo-Gold is a unique, patented, optimized cold storage and shipping media. It is designed to provide high quality of tissue or cell viability and functionality during storage at 2-8°C. This proprietary, high quality produced medium reduced the tissue and cell shock of cold storage situation. Thermo-Gold includes components that scavenger for free radicals, provide pH buffering, oncotic/osmotic support, energy substrates and electrolytes balance for plasma membrane stabilization due to membrane potential maintenance at cold storage condition. • Ready-to-use • Serum-free, protein-free • Animal component-free • cGMP manufactured with USP grade components • FDA master file • Sterility, endotoxin and cell-based tested Cryo-Preservation • Ready-to-use • Serum-free, protein-free • Animal component-free • Fully defined • cGMP available • Sterility, endotoxin and cell-based tested Cryo-Preservation is a process were cells or tissues are preserved using temperatures between -80°C to -196°C applying cryoprotectants. Many protocols exist for primary cells, stem cells and cell lines. We at PELOBiotech offer a broad range of different cryo-preservation media for different application and with different qualities. Many primary cells (adherent cells) are cryopreserved using the recommended culture medium plus 10-20% FBS and 10% DMSO. Reproducibility can become an issue as different FBS lots are used which normally were not tested for this application or cell type. In addition, FBS contains exosomes which will might have unwanted effects on cells and tissues. Immune cells as well as stem cells are mostly cryo-preserved in commercial cryomedia which are produced under GMP conditions. These cells are more fragile and a spontaneous differentiation of stem cells should be avoided. For ES and iPS cells DMSO-free cryo-media become more of interest to avoid the possible negative effects of DMSO. DMSO in general is a toxic agent and can prevent the "normal" differentiation of stem cells. This is one reason why many groups try to develop DMSO-free cryo-media. Beside DMSO-containing cryo media we offer DMSO-free media as well as protein-free cryo media for different applications. Figure 1: Process flow chart cryo-preservation 33