Products at a Glance 2024

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www.pelobiotech.com Further products of interest: BloodStor® The storage of stem cells from cord blood, peripheral blood, and bone marrow is crucial due to their potential therapeutic uses in treating various diseases. Cord blood, in particular, contains hematopoietic stem cells (HSC) that can be used to treat conditions such as leukemia, lymphoma, sickle cell disease, and other blood, bone, immune, and metabolic disorders. Cord blood stem cells are being researched for their potential in regenerative medicine, where they may be used to induce healing and regenerate cells to restore tissues. Furthermore, peripheral blood and bone marrow are also valuable sources of stem cells for transplantation, with peripheral blood offering advantages such as easier and more common collection compared to bone marrow. Exploratory analyses have indicated that peripheral-blood stem cells may reduce the risk of graft failure, while bone marrow may reduce the risk of chronic graft-versus-host disease. Proper storage of these stem cells is essential to maintain their viability and functionality for future therapeutic use. Umbilical cord blood stem cells can be stored in cord blood banks for potential use in stem cell transplants, providing an "off-theshelf" source of stem cells unlike bone marrow. The ability to store umbilical cord stem cells for future use presents a significant clinical advantage. Moreover, the use of stem cells aspirated from bone marrow eliminates the need for the creation of a second surgical site for harvesting autogenous bone, highlighting the importance of proper storage and preservation of these cells. ThawStar® ThawStar® offers reproduceable thawing of cells this is important as studies have shown that cryopreserved cells, such as mesenchymal stromal cells (MSCs), display impaired immunomodulatory properties and reduced therapeutic potential immediately after thawing. This impairment can lead to faster complement-mediated elimination after exposure to blood, indicating a compromised functionality of the cells. The cryopreservation process has been found to affect the viability and metabolic function of cells, making them unsuitable for clinical use upon thawing. The alteration of the actin cytoskeleton due to cryopreservation can also impact cell function and survival post-thawing. The susceptibility of cryopreserved MSCs to T-cell mediated apoptosis and their altered biodistribution in vivo after thawing further emphasize the detrimental effects of improper thawing on cell functionality. Moreover, the impact of cryopreservation on cell migration ability and the alteration of the functional competency and survival of cells due to freezing and thawing further highlight the importance of proper thawing procedures to maintain cell functionality. It is evident that the processing, freezing, and thawing cycle can be detrimental to the cells, emphasizing the need for optimized thawing protocols to preserve cell potency and therapeutic effect. Furthermore, the use of cryopreserved products immediately after thawing has been associated with a higher failure rate compared to the use of fresh cells, underscoring the critical nature of proper thawing procedures. Cell Culture Supplements Human and Bovine Serum Products: • HSA Solution 25% (cGMP): Human Serum Albumin (HSA) is a versatile product used in various cell culture applications. It serves as a stabilizer for enzymes, a blocking agent in Western blots, and a component of cell culture media. Its high purity ensures consistent performance in diverse assays. • Human AB Serum, Converted from Octaplas®: This converted human AB serum is crucial for culturing primary cells, including hematopoietic cells and stem cells. It's xeno-free and virus-inactivated, making it suitable for a wide range of cell culture applications. • Human Serum Albumin (HSA) 25% CSS (cGMP): Closed System Solutions (CSS) of HSA is designed for biomanufacturing applications, such as the production of vaccines, therapeutic proteins, and cell therapies. • Bovine Serum Albumin (Fraction V): Bovine Serum Albumin (BSA) is a common protein supplement in cell culture media. It stabilizes enzymes and acts as a carrier protein for hormones, fatty acids, and other compounds. BSA is often used in immunocytochemistry, ELISA, and Western blotting. 36

www.pelobiotech.com • Fetal Bovine Serum (FBS): FBS is a classic cell culture supplement known for its growth-promoting properties. It is used for the cultivation of a wide variety of cell types, including primary cells and established cell lines. FBS is a rich source of growth factors and nutrients. • Fetal Bovine Serum, Heat-Inactivated: Heat-inactivated FBS is treated to inactivate complement, making it suitable for specific cell culture applications where complement activity needs to be minimized. Growth Factors and Supplements • Endothelial Cell Growth Supplement (ECGS): ECGS is essential for the growth of endothelial cells and is often used in angiogenesis and vasculogenesis studies. • L-Glutamine-MAXIMUM Solution (100x): L-Glutamine is an amino acid vital for cell growth, and the MAXIMUM Solution ensures its stability in cell culture. • ITS Solution 100x: Containing insulin, transferrin, and selenium, ITS Solution enhances cell growth and is commonly used in serum-free media and bioprocessing applications. • UltraGRO Advanced Cell Culture Supplement: This advanced supplement, derived from human platelet lysate, promotes cell growth, expansion, and differentiation. It is suited for research and clinical applications, including regenerative medicine and tissue engineering. Exosome-Depleted Supplements Exosome-Depleted UltraGRO-PURE GI Research grade: A specialized supplement that excludes exosomes for research applications where exosome presence might affect the results. Xeno-Free and Defined Supplements • UltraGRO-PURE Cell Culture Supplement: A xeno-free supplement derived from human platelet lysate for applications where completely animal-free conditions are required. • Artificial Serum (Xeno-free): A xeno-free alternative to traditional serum, suitable for various cell culture experiments. • Artificial Serum (Animal-free): This animal-free supplement is ideal for researchers aiming to eliminate animal-derived components from their culture media. • Serum-Free RocketFuel: A serum-free medium supplement that provides essential nutrients for cell growth. • Serum-Free Recombinant RocketFuel: A serum-free and recombinant supplement designed for improved growth conditions. • BIT Admixture 100: A serum substitute that supports cell growth while eliminating the need for traditional serum. • Serum Replacement Supplement (Animal-free & Defined): An animal-free, defined supplement for researchers working with highly controlled conditions, including stem cell culture and regenerative medicine. Specific Tissue Extracts • Bovine Brain Extract (BBE): Bovine brain extract is used for specific cell culture applications, particularly in the neuroscience field. • Bovine Brain Extract-Hammond (BBE-Hammond): Hammond's formula BBE is a specialized supplement used for culturing neuronal cells. • Bovine Pituitary Extract (BPE)-Hammond: BPE is used in specific cell culture applications, especially for the growth of certain cell types. Miscellaneous Supplements • CultureBoost: A general-purpose cell culture supplement. • CultureBoost-R: Another cell culture supplement for promoting cell growth and health. • NEAA Cell Culture Supplement: Non-essential amino acids supplement that supports cell culture. 37