Products at a Glance 2024

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www.pelobiotech.com • B28 Neuron Culture Supplement: Specifically formulated for neuronal cell culture, aiding in the growth and differentiation of neurons. • Neural Cell Supplement: Designed for neural cell culture, particularly primary neuronal and glial cell cultures. • Fetal Bovine Serum EU approved (Cell culture tested): FBS that complies with European Union regulations, ensuring quality and consistency. • ECGF Bovine (Crude Extract, cell culture grade): A crude extract of bovine endothelial cell growth factor used for promoting angiogenesis and vasculogenesis in cell culture. These products are crucial for maintaining, expanding, and experimenting with various cell types, and they find applications in diverse fields such as regenerative medicine, stem cell research, tissue engineering, drug development, and fundamental cell biology studies. Extracellular Matrices Cell Culture experiments are very often done under relative strange conditions as important factors like stiffness and cell environment is not reflected in the chosen experimental designs. In the last 10 years cell culture improved a lot as the cellular microenvironment, surface stiffness and physiologic culture conditions are more taken into consideration. Coating of cell culture surfaces is done in most experiments to increase cell attachment and cell spreading. Used are un-defined mixtures of extracellular proteins derived from various sources. We offer ready-to-use ECM solutions from different sources, gelatin and purified extracellular matrix proteins. The MicroMatrix Array gives you the chance to evaluate the best matrix composition for your cellular systems on a single slide. 36 different ECM mixtures are used which are plotted 9 times per square. The assay was used so far for cell adherence and stem cell differentiation experiments. MicroMatrix 36 | MicroMatrix 36-4Pack | Cat#: PB-MM012011 – 1 slide Cat#: PB-MM012011-4 – 4 slides Pic: Micromatrix at work Gelatin & Coating mixtures We offer various ECM mixtures to coat the culture vessels to enhance cell attachment, cell spreading and cell growth. • Gelatin • Poly-D-Lysine & Poly-L-Lysine • Gelatin & Poly-L-Lysine • EHS-Matrix derived from mouse Engelbreth-Holm-Swalm tumor cells; the EHS matrix is widely used for stem cells, organoid and spheroid cultures • ExtraGel an optimized and effective growth scaffold for organoid, pluripotent stem cell, and advanced 3D culture applications. • Neuron Plating Solution • Speed Coating Solution • Stem Cell Matrix XF for pluripotent stem cells 38

www.pelobiotech.com Organ derived NativeCoat TM – 2D ECM Surface Coatings Coat any cell culture surface with NativeCoat ECM to give your cells the full milieu of proteins and growth factors present in the native tissue of interest. Available are the following products derived from healthy porcine acellular organs: • NativeCoat Blood Vessel ECM • NativeCoat Bone ECM • NativeCoat Cartilage ECM • NativeCoat Colon ECM • NativeCoat Heart ECM • NativeCoat Intestine ECM • NativeCoat Kidney ECM • NativeCoat Liver ECM • NativeCoat Lung ECM • NativeCoat Pancreas ECM • NativeCoat Skin ECM • NativeCoat Stomach ECM Just call us, if you need further information! Biomatrix Proteins Beside ECM mixtures we also offer purified biomatrix proteins derived from various sources. • Collagen Type I, II, III, IV • Fibronectin (human plasma derived; also available in GMP quality and virus inactivated) • iMatrix 221 (Recombinant Laminin for Cardiomyocytes & Myoblast) • iMatrix 411 (Recombinant Laminin for Endothelial Cells) • iMatrix 511 (Recombinant Laminin for ES/iPS Cells) • Vitronectin ExtraGel – the equivalent of Matrigel ® Scilia Extragel represents a reconstituted matrix hydrogel derived from basement membrane components sourced from mouse tumor tissues, notably abundant in extracellular matrix proteins. This matrix hydrogel primarily consists of laminin, collagen IV, and heparan sulfate proteoglycans. It also incorporates essential growth factors such as epidermal growth factor, platelet-derived growth factors, nerve growth factors, basic fibroblast growth factor, transforming growth factorbeta, and insulin-like growth factors. • Rigorously tested for LDEV, fungi, bacteria, and mycoplasma, Scilia Extragel offers a convenient laboratory solution devoid of pollutants. • Our commitment to quality is evident through strict inspections to ensure product stability, consistency, and reliable performance across batches. • Compatible with a variety of cell culture media, this hydrogel allows for versatile applications. • With a single-batch manufacturing process capable of yielding up to 1L volume, Scilia Extragel guarantees a steady and substantial supply for your research needs. Hydrogels Hydrogels can be used for 2D & 3D cell culture as shown in the pictures below: Pic: Cells growing …on top of hydrogel| 39 … in a hydrogel (3D Cell Culture)