Products at a Glance 2024

www.pelobiotech.com 3D

www.pelobiotech.com 3D Cell Culture Welcome to your 3D One-Stop-Cell Culture Shop, Competence in cell culture is PELOBiotech's standout feature. We aim to provide you with the best and most reliable results, allowing you to work with confidence and ease. At PELOBiotech, we take pride in our successful journey with 3D technologies. With one of our founders having contributed to the development and establishment of various systems now leading in the 3D industry, we are well-equipped to assist you in finding optimal solutions for your research. That's why we maintain a wide array of 3D technologies, providing ready-to-use 3D cell culture models and cell culture assay kits. We are continually exploring innovative 3D technologies for spheroid and organoid formation. We offer different 3D Technologies and 3D Models • Scaffold-free • Scaffold-based • Hydrogel-based 3D CoSeedis TM EZ-Seed ABLE ® Biott ® Bioreactors EZSPHERE TM Multi-Well Plates and Dishes (AGC ® ) Organotypic Scaffolds SEEDEZ - glass microfiber scaffolds Alvetex® Scaffolds SpheroSeev • 3D Models Ready-to-Use 3D Skin Model 3D Airway Model • Dynamic Models Organotypic Hydrogels isolated from different organs PLMA photopolymerizable hydrogel, platelet lysate derived MatriMix Collagen, Fibronectin Extragel an equivalent to Matrigel ® HydroSeev to improve your hydrogel culture Phenodrive Midi & Microfluidic Systems PerfusionPAL - Scaffold based Perfusion Systems Contact us today and let us show you how we can help you meet your technical and budget targets and bring your research from bench to bedside. 44

www.pelobiotech.com 3D Scaffold-free 3D CoSeedis The leading Swiss expert in Biostandardisation abc biopply ag has developed the innovative modular multi-purpose 3D Cell Co-Culture System 3D CoSeedis TM . Biostandardization respects clinical requirements already in the stage of molecular research and therefore can safe years in therapeutic development. With this unique and easy readout system a new standard in 3D Co-Culture is set. The novel scaffold-free 3D cell co-culture system consists of a unique conical agarose matrix array (CAMA) that allows the formation of spheroidal and non-spheroidal cell aggregates in a highly-reproducible and consistent manner. The conically shaped microwells within the array allow precise determination of aggregate volume and cell growth. Furthermore, the modular composition of the 3D system allows distance co-cultures and, consequently, the standardization of protocols. Key Features Matrix and Modular Set-up • Contact or distance co-cultures (fully separable) • Serum, serum-reduced or serum-free conditions possible Permeable Matrix • Allows long term 3D culture of cell aggregates • Efficient use of physically separated feeder cells (distance co-culture) • Cultivation in standard or defined media Multi-purpose Applicability • Applicable to various cell types (see Applicable Cell Systems) • Supports the formation of aggregates of spheroidal and non-spheroidal cell types • Highly reproducible cell aggregates • Suitable for high-throughput applications Unique and easy Readout • Integrated method for histological analysis (H&E, IHC, ISH, etc.) 3D CoSeedis is validated for the following applications: • 3D growth monitoring by volumetric analysis • Life/dead TM • Viability assays • HTS/HCS • Automated microscopy • Immunohistochemistry and -fluorescence • RNA, DNA and protein profiling • Extracellular vesicle production/profiling (e.g. EV-free mesenchymal stromal/stem cell co-cultures) • Whole-mount staining • Colony forming assay for tumorsphere formation and clonogenic survival Mass production of homogenous and uniform organoids/spheroids by using 3D CoSeedis TM One of the most important topics of 3D cell culture is a smooth and robust transition of 2D cell culture to 3D. This will help to create standardized workflows and protocols which are the fundament of reproducible and consistent results. abc biopply 45