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FLEISCHWIRTSCHAFT international 1/2017

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54<br />

Fleischwirtschaft <strong>international</strong> 1_<strong>2017</strong><br />

Research &Development<br />

Outlook<br />

Standardised techniques are available for differentiating mammal, fish<br />

and crustacean species. The techniques are primarilyqualitative in<br />

character.Inmixtures the detection limits are approx. 0.1to1%.<br />

Methods that include differentiation by means of conventional sequence<br />

analysis allow abroad species differentiation. However, these<br />

methods are less suitable for differentiation in mixtures. There have<br />

been some developments here in the field of NGS in recent years that will<br />

allow the use of NGS methods for analysing mixed samples in routine<br />

work as well in future. Accordinglysome service laboratories are already<br />

offering this analysis method.<br />

For screening of abroader spectrum of animal species, acommercial<br />

system on the basis of aDNA chip is also suitable.<br />

LC-MS/MS methods for animal species differentiation have not yet<br />

been adopted in routine analysis, but they certainlyhave potential for<br />

this. Further developments in this field remain to be awaited, and the<br />

same applies for Digital PCR (ddPCR).<br />

The techniques of animal species determination via multiplex realtime<br />

PCR now included in the Official Collection can be used, especially<br />

with suitable matrix calibrators, in asemi-quantitative manner as well<br />

and thus for separating unavoidable trace contamination (

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