Appendix A Standard Operating Procedures - University of Rhode ...

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4 of 4 3.2.3 Plasticware & All Bottle Caps Cleaning Procedure Plasticware is used to hold samples for pH, alkalinity, chlorophyll filtration, total suspended solids (TSS), biological oxygen demand (BOD) and/or microbial analysis. This plasticware is never soaked in acid to minimize the potential for inadvertently acidifying the water samples. Bottles utilized in the microbiological assays are autoclaved after cleaning. The procedure for autoclaving plastic bottles for microbial analysis can be found in SOP 005 - Bottle Autoclaving Procedure. Bottle caps, especially those for glass bottles, are never soaked in acid because it may damage the surface of the cap. Plasticware and bottle caps are cleaned using the procedure outlined below: 1. Follow steps 1 through 7 of the labware cleaning procedure. 2. Follow steps 12 through 14 of the labware cleaning procedure. General Labware Cleaning Procedure SOP 003 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 003 GlasswareWashing-MOD.doc
Standard Operating Procedure 004 General Autoclave Operation University of Rhode Island Watershed Watch 1.0 PURPOSE AND DESCRIPTION Date: Revision: Author: 08/08 3 Elizabeth Herron The autoclave is used to sterilize materials used for bacterial analysis. The procedure outlined below is for the autoclave in Coastal Institute only. This autoclave is sometimes referred to as the “NRS autoclave”. 2.0 HEALTH AND SAFETY CONSIDERATIONS Be aware that materials that have been autoclaved will be extremely hot when the cycle is completed. Wear insulated gloves when handling materials. 3.0 AUTOCLAVE OPERATION 3.1 Overview & Maintenance Before operating the autoclave please take a few minutes to read through the operator’s manual. It is very important that the unit and area around it be kept clean, and that materials to be autoclaved are appropriate for this unit and properly packaged. The door to the autoclave should be kept open when it is not in use, with the power cord unplugged. Please complete the log sheet with each run (a sample log sheet is attached below). THE EXACT MATERIALS PLACED INTO THE AUTOCLAVE MUST BE RECORDED (ie: filter assemblies, pipettes, media, etc.)_Also be sure that required maintenance has been performed. Elizabeth Herron (room 001F, 4-4552) is responsible for maintaining the unit, contact her if there are any questions regarding the operation or maintenance of the autoclave. 3.2 Operation The instructions below are to be used for the autoclave in the Kingston Coastal Institute only. 1. Place items to be sterilized on the appropriate tray (see operators manual), and put into the autoclave, leaving the door open. 2. Make sure that the maximum temperature recording thermometer is in the autoclave and the mercury is shaken down below 100 °C. 3. Ensure that the water reservoir is filled to BOTTOM of the safety value. If not, add deionized (DI) water, being sure not to immerse the safety valve. 4. Plug the power cord in, and turn the main switch to START. 1 of 4 General Autoclave Operation SOP 004 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 004 GenAutoclaveOps-MOD.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5 and 6: SUBJECT: Hazardous Materials and Ch
Page 7 and 8: 2.0 METHOD USED TO OBTAIN ULTRAPURE
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11: 3.2.2 Full Description of Labware C
Page 15 and 16: 4. Open the door and remove the loa
Page 17 and 18: Standard Operating Procedure 005 Bo
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21 and 22: 7. Close the door, and lock it by t
Page 23 and 24: Standard Operating Procedure 007 (P
Page 25 and 26: Several chemicals are utilized in t
Page 27 and 28: Required Material Notes Re-order in
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41 and 42: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 43 and 44: Dil. (mLs): The volume of sample fi
Page 45 and 46: Bacterial Sample Log & Worksheet: B
Page 47 and 48: Standard Operating Procedure 008 (P
Page 49 and 50: handling these chemicals. Material
Page 51 and 52: Required Material 4 of 22 Notes Re-
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
Page 57 and 58: 7. While the mTEC media is autoclav
Page 59 and 60: 12 of 22 5.3.2.3 Preparation of 1N
Page 61 and 62: 4. Label the bottom (half holding t
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20. Transfer the plates to the 44.5
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8.0 DOCUMENTATION Example Project D
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URI Watershed Watch Laboratory Quar
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Date 22 of 22 URI Watershed Watch L
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Standard Operating Procedure 009 (P
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Disposal Samples are archived for a
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3. Aluminum weigh dishes, pans, and
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a. If a sample clogs a filter, remo
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8.0 DOCUMENTATION Example Data Shee
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4.2 pH and Alkalinity Matrix Sample
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are stored in bulk in the cabinet a
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5.3.3 Procedure - Analysis of pH on
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5. Use a non-acid washed graduated
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8.0 DOCUMENTATION Data sheet for an
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3.0 REQUIRED MATERIALS Required Mat
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field blank water and 100 mL of the
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procedure. The DO probe should not
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5.3.2 Procedure - Day of Sample Col
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13. The water seal of the BOD bottl
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7.0 REFERENCES 100 - 350 350 - 500
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14 of 14 BI - 12 Fla STE -S 5 BI -
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Required Material Notes Re-order in
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Filter Blank Filter blanks consist
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8. When the fluorometer calls for t
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5.2.5 Calibration Check/Laboratory
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6. Check the calibration of the rep
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5.3.2.2 Preparation of 100 mL of 1N
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5.3.3.1 Procedure for Diluting Off-
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7.0 REFERENCES APHA, AWWA, WEF. Sta
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Chlorophyll-a Calibration Record Ca
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Chlorophyll-a Analysis Data Sheet N
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samples are analyzed until an accep
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5.3.1.1 Preparation of 1000 ppm NaC
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2. Prepare a filter housing by plac
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2. Pipette 100 µl of the chloride
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parts and eyes. Sulfuric Acid (H2SO
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The available volume of each reagen
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Corrective Action If the %D is grea
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. Values returned by the balance sh
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5. Shake the unfiltered sample bott
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5.3.2.3 Sample Analysis Detailed in
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2.0 HEALTH AND SAFETY CONSIDERATION
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6 of 19 5.2.1 Method Detection Limi
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and the samples analyzed between th
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10 of 19 serviced and re-calibrated
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X 20 400 X 25 500 X 50 1000 (1.0 mL
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they must be placed in the freezer
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Th ese instruction allow for the cr
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6. After the computer results are p
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USample Log Sheet SR1 SYNC (High st
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1 of 25 Standard Operating Procedur
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Corrective Action If any method (di
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Corrective Action If the %D is grea
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The percent recovery is calculated
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All phosphorus glassware should be
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adjustable pipette to add intermedi
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Preparation of Nitrate/Nitrite Work
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c. Enter SPK2 for the spike number
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Digesting Reagent for Total Phospho
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. The standard curve is rejected if
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Sample Log Sheet SR1 SYNC (High std
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Standard Operating Procedure 017 Sa
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5.2.3 Sample Replication Sample rep
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Sample Data Sheet Analysis Tech's R
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Salinity Analysis SOP 017 S:\WW\aww
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Standard Operating Procedure 018 En
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4.0 SAMPLE STORAGE, PRESERVATION AN
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eanalyzed. The field samples will b
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c. Dilute to the 100 mL mark on the
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7.0 CALCULATIONS Record the number
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11 of 21 Enterolert SOP 018 filenam
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Sample Bottle Sterility Check Bacte
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URI Watershed Watch Laboratory Quar
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Figure 1: Balance Calibration Data
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6.0 DOCUMENTATION 4 of 5 Bacterial
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Appendix A Standard Operating Procedures - University of Rhode ...

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