Appendix A Standard Operating Procedures - University of Rhode ...

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URI Watershed Watch Laboratory Quarterly UV germicidal Tube Record Date Tube # Intensity Date Tube # Intensity Initial intensity 1 4 readings 2 5 3 6 Date Tech's Initials Tube # Reading (right side) Reading (center) Reading (left side) Average Reading Wear protective gear when measuring UV lamp intensity! Intensity Reduction Comments / Notes This form is intended to be used to maintain a quarterly record of the intensity of the germicidal UV labs in the sterilzing box in room 19. The intensity of EACH of the SIX tubes must be measured on a quarterly basis, with each tube measured four inches from each end, as well as in the center, wtih the average of those three readings used. Once this sheet is full it should be replaced with a new blank sheet. The full sheet should be returned to the URIWW Micro Laboratory Binder in Room 019; blank sheets are also located in this binder. Any maintenance activity should also be recorded on this sheet. See Elizabeth with questions. 21 of 23 Ambient Waters Microbiological Procedure SOP 007 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 007AmbientMicrobes.doc
Bacterial Sample Log & Worksheet: Bacteria Bottle Sterilization Confirmation mTEC Membrane Filtration Method Analyst - set-up: Analyst - Counts: Incubator temp. start: Waterbath temp. start: incubator temp. end: Waterbath temp. end: Setup Dil. 24 Hr Total fecal Monitoring Location Date (mls) Count (per 100ml) Comments Batch #4 100 Batch #5 100 Analyst - set-up: Enterolert IDEXX Method Analyst - Counts: Media Batch #: Incubator temp. start: Incubator temp. end: Notes: "-" = Quanti-tray used, no small wells Expiration: Final count determined by using the correct table (51 well Quanti-Tray lg wells only, Quanti-Tray / 2000 with small wells) 22 of 23 Setup Dil. # lg pos. # sm pos. Table Enterococci Date (mls) wells wells Value Batch #4 100 - Batch #5 100 - IDEXX Blank 100 - URIWW Sterilized 100 - Ambient Waters Microbiological Procedure SOP 007 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 007AmbientMicrobes.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5 and 6: SUBJECT: Hazardous Materials and Ch
Page 7 and 8: 2.0 METHOD USED TO OBTAIN ULTRAPURE
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11 and 12: 3.2.2 Full Description of Labware C
Page 13 and 14: Standard Operating Procedure 004 Ge
Page 15 and 16: 4. Open the door and remove the loa
Page 17 and 18: Standard Operating Procedure 005 Bo
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21 and 22: 7. Close the door, and lock it by t
Page 23 and 24: Standard Operating Procedure 007 (P
Page 25 and 26: Several chemicals are utilized in t
Page 27 and 28: Required Material Notes Re-order in
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41 and 42: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 43: Dil. (mLs): The volume of sample fi
Page 49 and 50: handling these chemicals. Material
Page 51 and 52: Required Material 4 of 22 Notes Re-
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
Page 57 and 58: 7. While the mTEC media is autoclav
Page 59 and 60: 12 of 22 5.3.2.3 Preparation of 1N
Page 61 and 62: 4. Label the bottom (half holding t
Page 63 and 64: 20. Transfer the plates to the 44.5
Page 65 and 66: 8.0 DOCUMENTATION Example Project D
Page 67 and 68: URI Watershed Watch Laboratory Quar
Page 69 and 70: Date 22 of 22 URI Watershed Watch L
Page 73 and 74: Disposal Samples are archived for a
Page 75 and 76: 3. Aluminum weigh dishes, pans, and
Page 77 and 78: a. If a sample clogs a filter, remo
Page 79 and 80: 8.0 DOCUMENTATION Example Data Shee
Page 83 and 84: 4.2 pH and Alkalinity Matrix Sample
Page 85 and 86: are stored in bulk in the cabinet a
Page 87 and 88: 5.3.3 Procedure - Analysis of pH on
Page 89 and 90: 5. Use a non-acid washed graduated
Page 91 and 92: 8.0 DOCUMENTATION Data sheet for an
Page 95 and 96:
3.0 REQUIRED MATERIALS Required Mat
Page 97 and 98:
field blank water and 100 mL of the
Page 99 and 100:
procedure. The DO probe should not
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5.3.2 Procedure - Day of Sample Col
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13. The water seal of the BOD bottl
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7.0 REFERENCES 100 - 350 350 - 500
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14 of 14 BI - 12 Fla STE -S 5 BI -
Page 109 and 110:
Standard Operating Procedure 012 (P
Page 111 and 112:
Required Material Notes Re-order in
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Filter Blank Filter blanks consist
Page 115 and 116:
8. When the fluorometer calls for t
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5.2.5 Calibration Check/Laboratory
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6. Check the calibration of the rep
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5.3.2.2 Preparation of 100 mL of 1N
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5.3.3.1 Procedure for Diluting Off-
Page 125 and 126:
7.0 REFERENCES APHA, AWWA, WEF. Sta
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Chlorophyll-a Calibration Record Ca
Page 129 and 130:
Chlorophyll-a Analysis Data Sheet N
Page 131 and 132:
Page 133 and 134:
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samples are analyzed until an accep
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5.3.1.1 Preparation of 1000 ppm NaC
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2. Prepare a filter housing by plac
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2. Pipette 100 µl of the chloride
Page 143 and 144:
Page 145 and 146:
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parts and eyes. Sulfuric Acid (H2SO
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The available volume of each reagen
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Corrective Action If the %D is grea
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. Values returned by the balance sh
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5. Shake the unfiltered sample bott
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5.3.2.3 Sample Analysis Detailed in
Page 159 and 160:
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2.0 HEALTH AND SAFETY CONSIDERATION
Page 165 and 166:
Page 167 and 168:
6 of 19 5.2.1 Method Detection Limi
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and the samples analyzed between th
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10 of 19 serviced and re-calibrated
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X 20 400 X 25 500 X 50 1000 (1.0 mL
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they must be placed in the freezer
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Th ese instruction allow for the cr
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6. After the computer results are p
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USample Log Sheet SR1 SYNC (High st
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1 of 25 Standard Operating Procedur
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Corrective Action If any method (di
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Corrective Action If the %D is grea
Page 193 and 194:
The percent recovery is calculated
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All phosphorus glassware should be
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adjustable pipette to add intermedi
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Preparation of Nitrate/Nitrite Work
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c. Enter SPK2 for the spike number
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Digesting Reagent for Total Phospho
Page 205 and 206:
. The standard curve is rejected if
Page 207 and 208:
Sample Log Sheet SR1 SYNC (High std
Page 209 and 210:
Standard Operating Procedure 017 Sa
Page 211 and 212:
5.2.3 Sample Replication Sample rep
Page 213 and 214:
Sample Data Sheet Analysis Tech's R
Page 215 and 216:
Salinity Analysis SOP 017 S:\WW\aww
Page 217 and 218:
Standard Operating Procedure 018 En
Page 219 and 220:
4.0 SAMPLE STORAGE, PRESERVATION AN
Page 221 and 222:
eanalyzed. The field samples will b
Page 223 and 224:
c. Dilute to the 100 mL mark on the
Page 225 and 226:
7.0 CALCULATIONS Record the number
Page 227 and 228:
11 of 21 Enterolert SOP 018 filenam
Page 229 and 230:
Page 231 and 232:
Sample Bottle Sterility Check Bacte
Page 233 and 234:
URI Watershed Watch Laboratory Quar
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Figure 1: Balance Calibration Data
Page 241 and 242:
Page 243:
6.0 DOCUMENTATION 4 of 5 Bacterial
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