Appendix A Standard Operating Procedures - University of Rhode ...

More documents

Recommendations

Info

Required Material Pipette-Aid filler/dispenser (electric) 2 - Glass 400 mL beakers marked URIWW 4 - Side arm filter flasks 500 mL or larger 3 of 22 Notes Re-order information Fisher catalog # 13-681-15 Thermolyne Vortex Mixer Fisher catalog #12-814 Coastal Institute in Kingston, room 019, URIWW refrigerator mTEC media plates Must be less than a month old, stored media side up in a foil lined box in the refrigerator mTEC media mix Fisher catalog # DF0334- 15-0 Agar plate with E. coli Blank media plates Tryptic soy broth tubes QC sample Agar mix Fisher catalog #BP1423- 500 Tryptic soy broth mix Fisher catalog #DF0370- 17-3 Stock KH2PO4 solution Preparation instructions: Section 5.3.2.4 Stock MgCl2 solution Preparation instructions: Section 5.3.2.5 Sticks - sterilized Fisher catalog #01-340 Coastal Institute in Kingston, room 019, Supplies in or on the gray table to the left of countertop 95% Ethanol in a 500 mL plastic bottle Alcohol lamp Fisher catalog #04-245-1 Envirocide or Conflict Disinfectant solution in squirt bottle Fisher Catalog #04-324-12 Filter forceps Small beaker (~ 50 mL) Indelible marker (Sharpie) Matches Fisher catalog #09-753-30 Magnetic filter funnels (9 – 47 mm diameter, Gelman Fisher #09-735 hanging from drying rack on #4242, 300 mL capacity, 50 incubator) mL gradations Sterile buffer filled Nalgene wash Fisher catalog # 03-409bottles 14C Sterile grid membrane filters Fisher catalog # 09-719-1B or HAWG 047 S1 ISDS Microbiological Analysis SOP 008 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 008 ISDSMicrobes.doc
Required Material 4 of 22 Notes Re-order information Coastal Institute in Kingston, room 019, Plastic drawers or boxes beneath the lab countertop Sterile plastic Petri dishes Fisher catalog # 08-757-19 Sterile 1 mL pipettes Fisher catalog #13-678- 25C Sterile 10 mL pipettes Fisher catalog #13-678- 25F Sterile 25 mL pipettes Fisher catalog #13-676- 29D Pipette sterilizing pouches Fisher catalog # 01-812-53 Coastal Institute in Kingston, room 019, Drawer or shelf beneath the lab countertop Reusable test tubes Fisher catalog # 14-925J Test tube rack Aluminum foil Fisher catalog # 14-809-24 Coastal Institute in Kingston, room 019, In cabinet beneath the water bath Whirl-pak TM bags Fisher catalog # 01-812- 5C Clear biohazard autoclave bags Fisher catalog #01-826-5 Coastal Institute in Kingston, room 019, On shelf above the water bath Plastic carboy filled with Preparation instructions: phosphate buffer saline solution Section 5.3.2.1 and 5.3.2.2 Coastal Institute in Kingston, room 019, In the upper cabinet Phenol red Sigma catalog #P2417 Sodium chloride Fisher catalog # S671-500 Magnesium chloride hexahydrate (MgCl2·6H2O) Fisher catalog # M33-500 Potassium phosphate monobasic (KH2PO4) Fisher catalog # P382-500 1 N Sodium Hydroxide (NaOH) Causes eye, skin, digestive and respiratory tract burns. Caustic. Preparation instructions: Section 5.3.2.3. Coastal Institute in Kingston, room 019, In the chemical cabinet 95% Ethanol in a 2 L glass bottle Available through the Agricultural Experiment Station office in Woodward Hall Equipment is maintained by the Watershed Watch Laboratory and the Natural Resources Science department. Temporary replacement of large equipment such as incubators is available through arrangement with other scientists in the department maintaining similar equipment. ISDS Microbiological Analysis SOP 008 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 008 ISDSMicrobes.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5 and 6: SUBJECT: Hazardous Materials and Ch
Page 7 and 8: 2.0 METHOD USED TO OBTAIN ULTRAPURE
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11 and 12: 3.2.2 Full Description of Labware C
Page 13 and 14: Standard Operating Procedure 004 Ge
Page 15 and 16: 4. Open the door and remove the loa
Page 17 and 18: Standard Operating Procedure 005 Bo
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21 and 22: 7. Close the door, and lock it by t
Page 23 and 24: Standard Operating Procedure 007 (P
Page 25 and 26: Several chemicals are utilized in t
Page 27 and 28: Required Material Notes Re-order in
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41 and 42: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 43 and 44: Dil. (mLs): The volume of sample fi
Page 45 and 46: Bacterial Sample Log & Worksheet: B
Page 49: handling these chemicals. Material
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
Page 57 and 58: 7. While the mTEC media is autoclav
Page 59 and 60: 12 of 22 5.3.2.3 Preparation of 1N
Page 61 and 62: 4. Label the bottom (half holding t
Page 63 and 64: 20. Transfer the plates to the 44.5
Page 65 and 66: 8.0 DOCUMENTATION Example Project D
Page 67 and 68: URI Watershed Watch Laboratory Quar
Page 69 and 70: Date 22 of 22 URI Watershed Watch L
Page 73 and 74: Disposal Samples are archived for a
Page 75 and 76: 3. Aluminum weigh dishes, pans, and
Page 77 and 78: a. If a sample clogs a filter, remo
Page 79 and 80: 8.0 DOCUMENTATION Example Data Shee
Page 83 and 84: 4.2 pH and Alkalinity Matrix Sample
Page 85 and 86: are stored in bulk in the cabinet a
Page 87 and 88: 5.3.3 Procedure - Analysis of pH on
Page 89 and 90: 5. Use a non-acid washed graduated
Page 91 and 92: 8.0 DOCUMENTATION Data sheet for an
Page 95 and 96: 3.0 REQUIRED MATERIALS Required Mat
Page 97 and 98: field blank water and 100 mL of the
Page 99 and 100: procedure. The DO probe should not
Page 101 and 102:
5.3.2 Procedure - Day of Sample Col
Page 103 and 104:
13. The water seal of the BOD bottl
Page 105 and 106:
7.0 REFERENCES 100 - 350 350 - 500
Page 107 and 108:
14 of 14 BI - 12 Fla STE -S 5 BI -
Page 109 and 110:
Standard Operating Procedure 012 (P
Page 111 and 112:
Required Material Notes Re-order in
Page 113 and 114:
Filter Blank Filter blanks consist
Page 115 and 116:
8. When the fluorometer calls for t
Page 117 and 118:
5.2.5 Calibration Check/Laboratory
Page 119 and 120:
6. Check the calibration of the rep
Page 121 and 122:
5.3.2.2 Preparation of 100 mL of 1N
Page 123 and 124:
5.3.3.1 Procedure for Diluting Off-
Page 125 and 126:
7.0 REFERENCES APHA, AWWA, WEF. Sta
Page 127 and 128:
Chlorophyll-a Calibration Record Ca
Page 129 and 130:
Chlorophyll-a Analysis Data Sheet N
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
samples are analyzed until an accep
Page 137 and 138:
5.3.1.1 Preparation of 1000 ppm NaC
Page 139 and 140:
2. Prepare a filter housing by plac
Page 141 and 142:
2. Pipette 100 µl of the chloride
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
parts and eyes. Sulfuric Acid (H2SO
Page 149 and 150:
The available volume of each reagen
Page 151 and 152:
Corrective Action If the %D is grea
Page 153 and 154:
. Values returned by the balance sh
Page 155 and 156:
5. Shake the unfiltered sample bott
Page 157 and 158:
5.3.2.3 Sample Analysis Detailed in
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
2.0 HEALTH AND SAFETY CONSIDERATION
Page 165 and 166:
Page 167 and 168:
6 of 19 5.2.1 Method Detection Limi
Page 169 and 170:
and the samples analyzed between th
Page 171 and 172:
10 of 19 serviced and re-calibrated
Page 173 and 174:
X 20 400 X 25 500 X 50 1000 (1.0 mL
Page 175 and 176:
they must be placed in the freezer
Page 177 and 178:
Th ese instruction allow for the cr
Page 179 and 180:
6. After the computer results are p
Page 181 and 182:
USample Log Sheet SR1 SYNC (High st
Page 183 and 184:
1 of 25 Standard Operating Procedur
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Corrective Action If any method (di
Page 191 and 192:
Corrective Action If the %D is grea
Page 193 and 194:
The percent recovery is calculated
Page 195 and 196:
All phosphorus glassware should be
Page 197 and 198:
adjustable pipette to add intermedi
Page 199 and 200:
Preparation of Nitrate/Nitrite Work
Page 201 and 202:
c. Enter SPK2 for the spike number
Page 203 and 204:
Digesting Reagent for Total Phospho
Page 205 and 206:
. The standard curve is rejected if
Page 207 and 208:
Sample Log Sheet SR1 SYNC (High std
Page 209 and 210:
Standard Operating Procedure 017 Sa
Page 211 and 212:
5.2.3 Sample Replication Sample rep
Page 213 and 214:
Sample Data Sheet Analysis Tech's R
Page 215 and 216:
Salinity Analysis SOP 017 S:\WW\aww
Page 217 and 218:
Standard Operating Procedure 018 En
Page 219 and 220:
4.0 SAMPLE STORAGE, PRESERVATION AN
Page 221 and 222:
eanalyzed. The field samples will b
Page 223 and 224:
c. Dilute to the 100 mL mark on the
Page 225 and 226:
7.0 CALCULATIONS Record the number
Page 227 and 228:
11 of 21 Enterolert SOP 018 filenam
Page 229 and 230:
Page 231 and 232:
Sample Bottle Sterility Check Bacte
Page 233 and 234:
URI Watershed Watch Laboratory Quar
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Figure 1: Balance Calibration Data
Page 241 and 242:
Page 243:
6.0 DOCUMENTATION 4 of 5 Bacterial
show all

Appendix A Standard Operating Procedures - University of Rhode ...

Create successful ePaper yourself

Delete template?

Save as template?