Appendix A Standard Operating Procedures - University of Rhode ...

More documents

Recommendations

Info

Required Material Notes Re-order information Vacuum pump and manifold Pipette-Aid filler/dispenser (electric) 3 of 23 Fisher catalog # 13-681-15 2 - Glass 400 mL beakers marked URIWW 4 - Side arm filter flasks 500 mL or larger Coastal Institute in Kingston, room 019, URIWW refrigerator mTEC media plates less than a month old, stored media side up in a foil lined box in the refrigerator mTEC media mix Fisher catalog # DF0334-15-0 Agar plate with E. coli QC sample American Type Culture Collection # 35922 Tryptic soy broth (TSB) tubes Agar mix Fisher catalog #BP1423-500 Tryptic soy broth mix Fisher catalog #DF0370-17-3 Stock KH2PO4 solution Preparation instructions: Section 5.3.2.5 Stock MgCl2 solution Preparation instructions: Section 5.3.2.6 Urea substrate Preparation instructions: Section 5.3.2.3 Sticks - sterilized Fisher catalog #01-340 Coastal Institute in Kingston, room 019, Supplies in or on the gray table to the left of countertop 95% Ethanol in a 500 mL plastic bottle Alcohol lamp Fisher catalog #04-245-1 Envirocide or Conflict Disinfectant Fisher Catalog solution in squirt bottle #04-324-12 Filter forceps Small beaker (~ 50 mL) Fisher catalog #09-753-30 Indelible marker (Sharpie) Matches or igniter Magnetic filter funnels (9 – autoclaved and stored in sealed autoclave bags until used) Sterile buffer filled Nalgene wash bottles 47 mm diameter, Gelman #4242, 300 mL capacity, 50 mL gradations Fisher #09-735 Fisher catalog # 03-409-14C Ambient Waters Microbiological Procedure SOP 007 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 007AmbientMicrobes.doc
Required Material Notes Re-order information Sterile grid membrane filters Fisher catalog # 09-719-1B or HAWG 047 S1 Coastal Institute in Kingston, room 019, Plastic drawers or boxes beneath the lab countertop Sterile plastic Petri dishes Fisher catalog # 08-757-19 Sterile 1 mL pipettes Fisher catalog #13-678-25C Sterile 10 mL pipettes Fisher catalog #13-678-25F Sterile 25 mL pipettes Fisher catalog #13-676-29D Pipette sterilizing pouches Fisher catalog # 01-812-53 Coastal Institute in Kingston, room 019, Drawer or shelf beneath the lab countertop Reusable test tubes Fisher catalog # 14-925J Test tube rack Aluminum foil Fisher catalog # 14-809-24 Coastal Institute in Kingston, room 019, In cabinet beneath the water bath Whirl-pak TM bags Fisher catalog # 01-812-5C Clear biohazard autoclave bags Fisher catalog #01-826-5 Coastal Institute in Kingston, room 019, On shelf above the water bath Plastic carboy filled with Preparation instructions: Section 5.3.2.1 phosphate buffer saline solution and 5.3.2.2 Coastal Institute in Kingston, room 019, In the upper cabinet Urea Fisher catalog #DF0190-17-1 Phenol red Sigma catalog #P2417 Magnesium chloride hexahydrate Fisher catalog (MgCl2·6H2O) #M33-500 Potassium phosphate monobasic Fisher catalog (KH2PO4) #P382-500 Sodium chloride (NaCl) Fisher catalog # S671-500 1 N Sodium Hydroxide (NaOH) Causes eye, skin, digestive and respiratory tract burns. Caustic. Preparation instructions: 5.3.2.4. Coastal Institute in Kingston, room 019, In the chemical cabinet 95% Ethanol Fisher catalog # S93231 4 of 23 Ambient Waters Microbiological Procedure SOP 007 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 007AmbientMicrobes.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5 and 6: SUBJECT: Hazardous Materials and Ch
Page 7 and 8: 2.0 METHOD USED TO OBTAIN ULTRAPURE
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11 and 12: 3.2.2 Full Description of Labware C
Page 13 and 14: Standard Operating Procedure 004 Ge
Page 15 and 16: 4. Open the door and remove the loa
Page 17 and 18: Standard Operating Procedure 005 Bo
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21 and 22: 7. Close the door, and lock it by t
Page 23 and 24: Standard Operating Procedure 007 (P
Page 25: Several chemicals are utilized in t
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41 and 42: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 43 and 44: Dil. (mLs): The volume of sample fi
Page 45 and 46: Bacterial Sample Log & Worksheet: B
Page 49 and 50: handling these chemicals. Material
Page 51 and 52: Required Material 4 of 22 Notes Re-
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
Page 57 and 58: 7. While the mTEC media is autoclav
Page 59 and 60: 12 of 22 5.3.2.3 Preparation of 1N
Page 61 and 62: 4. Label the bottom (half holding t
Page 63 and 64: 20. Transfer the plates to the 44.5
Page 65 and 66: 8.0 DOCUMENTATION Example Project D
Page 67 and 68: URI Watershed Watch Laboratory Quar
Page 69 and 70: Date 22 of 22 URI Watershed Watch L
Page 73 and 74: Disposal Samples are archived for a
Page 75 and 76: 3. Aluminum weigh dishes, pans, and
Page 77 and 78:
a. If a sample clogs a filter, remo
Page 79 and 80:
8.0 DOCUMENTATION Example Data Shee
Page 81 and 82:
Standard Operating Procedure 010 (P
Page 83 and 84:
4.2 pH and Alkalinity Matrix Sample
Page 85 and 86:
are stored in bulk in the cabinet a
Page 87 and 88:
5.3.3 Procedure - Analysis of pH on
Page 89 and 90:
5. Use a non-acid washed graduated
Page 91 and 92:
8.0 DOCUMENTATION Data sheet for an
Page 93 and 94:
Page 95 and 96:
3.0 REQUIRED MATERIALS Required Mat
Page 97 and 98:
field blank water and 100 mL of the
Page 99 and 100:
procedure. The DO probe should not
Page 101 and 102:
5.3.2 Procedure - Day of Sample Col
Page 103 and 104:
13. The water seal of the BOD bottl
Page 105 and 106:
7.0 REFERENCES 100 - 350 350 - 500
Page 107 and 108:
14 of 14 BI - 12 Fla STE -S 5 BI -
Page 109 and 110:
Page 111 and 112:
Required Material Notes Re-order in
Page 113 and 114:
Filter Blank Filter blanks consist
Page 115 and 116:
8. When the fluorometer calls for t
Page 117 and 118:
5.2.5 Calibration Check/Laboratory
Page 119 and 120:
6. Check the calibration of the rep
Page 121 and 122:
5.3.2.2 Preparation of 100 mL of 1N
Page 123 and 124:
5.3.3.1 Procedure for Diluting Off-
Page 125 and 126:
7.0 REFERENCES APHA, AWWA, WEF. Sta
Page 127 and 128:
Chlorophyll-a Calibration Record Ca
Page 129 and 130:
Chlorophyll-a Analysis Data Sheet N
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
samples are analyzed until an accep
Page 137 and 138:
5.3.1.1 Preparation of 1000 ppm NaC
Page 139 and 140:
2. Prepare a filter housing by plac
Page 141 and 142:
2. Pipette 100 µl of the chloride
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
parts and eyes. Sulfuric Acid (H2SO
Page 149 and 150:
The available volume of each reagen
Page 151 and 152:
Corrective Action If the %D is grea
Page 153 and 154:
. Values returned by the balance sh
Page 155 and 156:
5. Shake the unfiltered sample bott
Page 157 and 158:
5.3.2.3 Sample Analysis Detailed in
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
2.0 HEALTH AND SAFETY CONSIDERATION
Page 165 and 166:
Page 167 and 168:
6 of 19 5.2.1 Method Detection Limi
Page 169 and 170:
and the samples analyzed between th
Page 171 and 172:
10 of 19 serviced and re-calibrated
Page 173 and 174:
X 20 400 X 25 500 X 50 1000 (1.0 mL
Page 175 and 176:
they must be placed in the freezer
Page 177 and 178:
Th ese instruction allow for the cr
Page 179 and 180:
6. After the computer results are p
Page 181 and 182:
USample Log Sheet SR1 SYNC (High st
Page 183 and 184:
1 of 25 Standard Operating Procedur
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Corrective Action If any method (di
Page 191 and 192:
Corrective Action If the %D is grea
Page 193 and 194:
The percent recovery is calculated
Page 195 and 196:
All phosphorus glassware should be
Page 197 and 198:
adjustable pipette to add intermedi
Page 199 and 200:
Preparation of Nitrate/Nitrite Work
Page 201 and 202:
c. Enter SPK2 for the spike number
Page 203 and 204:
Digesting Reagent for Total Phospho
Page 205 and 206:
. The standard curve is rejected if
Page 207 and 208:
Sample Log Sheet SR1 SYNC (High std
Page 209 and 210:
Standard Operating Procedure 017 Sa
Page 211 and 212:
5.2.3 Sample Replication Sample rep
Page 213 and 214:
Sample Data Sheet Analysis Tech's R
Page 215 and 216:
Salinity Analysis SOP 017 S:\WW\aww
Page 217 and 218:
Standard Operating Procedure 018 En
Page 219 and 220:
4.0 SAMPLE STORAGE, PRESERVATION AN
Page 221 and 222:
eanalyzed. The field samples will b
Page 223 and 224:
c. Dilute to the 100 mL mark on the
Page 225 and 226:
7.0 CALCULATIONS Record the number
Page 227 and 228:
11 of 21 Enterolert SOP 018 filenam
Page 229 and 230:
Page 231 and 232:
Sample Bottle Sterility Check Bacte
Page 233 and 234:
URI Watershed Watch Laboratory Quar
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Figure 1: Balance Calibration Data
Page 241 and 242:
Page 243:
6.0 DOCUMENTATION 4 of 5 Bacterial
show all

Appendix A Standard Operating Procedures - University of Rhode ...

Create successful ePaper yourself

Delete template?

Save as template?