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Appendix A Standard Operating Procedures - University of Rhode ...

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<strong>Standard</strong> <strong>Operating</strong> Procedure 007<br />

(Prior number URIWW-SOP-4A)<br />

Ambient Waters Microbiological<br />

Procedure<br />

<strong>University</strong> <strong>of</strong> <strong>Rhode</strong> Island Watershed Watch<br />

1.0 PURPOSE AND DESCRIPTION<br />

Date:<br />

Revision:<br />

Author:<br />

08/08<br />

3<br />

Elizabeth<br />

Herron<br />

The purpose <strong>of</strong> this method is to determine the concentration <strong>of</strong> Escherichia coli (E. coli) and<br />

fecal coliform bacteria within an ambient water sample. Samples are collected in sterile bottles,<br />

an aliquot <strong>of</strong> sample is filtered and the resulting filter placed into a media dish and incubated.<br />

After incubation the number <strong>of</strong> bacteria colonies are counted. This procedure is utilized for<br />

ambient water (lakes, ponds, rivers, etc.) only. Analysis <strong>of</strong> ISDS/septic samples is completed<br />

using SOP 008 - ISDS Microbiological Procedure. This method is applicable to undiluted<br />

samples in the range <strong>of</strong>

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