Appendix A Standard Operating Procedures - University of Rhode ...

More documents

Recommendations

Info

Standard Operating Procedure 007 (Prior number URIWW-SOP-4A) Ambient Waters Microbiological Procedure University of Rhode Island Watershed Watch 1.0 PURPOSE AND DESCRIPTION Date: Revision: Author: 08/08 3 Elizabeth Herron The purpose of this method is to determine the concentration of Escherichia coli (E. coli) and fecal coliform bacteria within an ambient water sample. Samples are collected in sterile bottles, an aliquot of sample is filtered and the resulting filter placed into a media dish and incubated. After incubation the number of bacteria colonies are counted. This procedure is utilized for ambient water (lakes, ponds, rivers, etc.) only. Analysis of ISDS/septic samples is completed using SOP 008 - ISDS Microbiological Procedure. This method is applicable to undiluted samples in the range of
Several chemicals are utilized in this SOP. Potassium phosphate monobasic (KH2PO4), magnesium chloride hexahydrate (MgCl2·6H2O), sodium chloride (NaCl) and Phenol red may cause skin irritation after prolonged exposure as well as irritation of eyes, nose and throat if inhaled or allowed to make contact with the eyes. Wear a lab coat, gloves and goggles when handling these chemicals. Material Safety Data Sheets (MSDS) are located in each laboratory for the materials stored in the specific laboratory. MSDS are contained in plastic file folders in rooms 019 and 018 and a notebook in room 002. 2.2 Technician Training/Qualifications General training in laboratory technique, use of an autoclave and sterile technique as well as specific training on the procedures contained in this method must be completed prior to analyzing samples. Technician training will be provided either by Elizabeth Herron (Laboratory Project Manager – Microbiology) or Linda Green (Laboratory Project Manager – Nutrients). 3.0 REQUIRED MATERIALS Required Material Notes Re-order information Coastal Institute in Kingston, room 081 Autoclave and maximum temperature thermometer University of Rhode Island (URI), Kingston Coastal Institute Watershed Watch (WW) Laboratory, room 002 Autoclave safe white plastic bottles (125 – 500 mL) 2 L autoclave safe flasks Insulated autoclave gloves Metal autoclave tray Combination hot plate and magnetic stirrer Magnetic stirring bar Microbiology worksheets Project data sheets are found on the URIWW computer. See Section 8.0 Documentation Sodium Hydroxide (NaOH) Caustic. Causes eye, skin, digestive and respiratory tract burns. Hygroscopic (absorbs moisture from the air). Coastal Institute in Kingston, room 018 Balance Calibration weights in drawer beneath the balance Coastal Institute in Kingston, room 019 35 C Incubator Thermolyne Type 142300 Incubator 44.5 C Water bath Precision fecal coliform bath 66855 Baxter catalog #W3182-2 Petri dish rack (for water bath) Baxter catalog #W3182-11 UV sterilization box 2 of 23 Ambient Waters Microbiological Procedure SOP 007 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 007AmbientMicrobes.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5 and 6: SUBJECT: Hazardous Materials and Ch
Page 7 and 8: 2.0 METHOD USED TO OBTAIN ULTRAPURE
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11 and 12: 3.2.2 Full Description of Labware C
Page 13 and 14: Standard Operating Procedure 004 Ge
Page 15 and 16: 4. Open the door and remove the loa
Page 17 and 18: Standard Operating Procedure 005 Bo
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21 and 22: 7. Close the door, and lock it by t
Page 23: Standard Operating Procedure 007 (P
Page 27 and 28: Required Material Notes Re-order in
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41 and 42: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 43 and 44: Dil. (mLs): The volume of sample fi
Page 45 and 46: Bacterial Sample Log & Worksheet: B
Page 47 and 48: Standard Operating Procedure 008 (P
Page 49 and 50: handling these chemicals. Material
Page 51 and 52: Required Material 4 of 22 Notes Re-
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
Page 57 and 58: 7. While the mTEC media is autoclav
Page 59 and 60: 12 of 22 5.3.2.3 Preparation of 1N
Page 61 and 62: 4. Label the bottom (half holding t
Page 63 and 64: 20. Transfer the plates to the 44.5
Page 65 and 66: 8.0 DOCUMENTATION Example Project D
Page 67 and 68: URI Watershed Watch Laboratory Quar
Page 69 and 70: Date 22 of 22 URI Watershed Watch L
Page 71 and 72: Standard Operating Procedure 009 (P
Page 73 and 74: Disposal Samples are archived for a
Page 75 and 76:
3. Aluminum weigh dishes, pans, and
Page 77 and 78:
a. If a sample clogs a filter, remo
Page 79 and 80:
8.0 DOCUMENTATION Example Data Shee
Page 81 and 82:
Standard Operating Procedure 010 (P
Page 83 and 84:
4.2 pH and Alkalinity Matrix Sample
Page 85 and 86:
are stored in bulk in the cabinet a
Page 87 and 88:
5.3.3 Procedure - Analysis of pH on
Page 89 and 90:
5. Use a non-acid washed graduated
Page 91 and 92:
8.0 DOCUMENTATION Data sheet for an
Page 93 and 94:
Page 95 and 96:
3.0 REQUIRED MATERIALS Required Mat
Page 97 and 98:
field blank water and 100 mL of the
Page 99 and 100:
procedure. The DO probe should not
Page 101 and 102:
5.3.2 Procedure - Day of Sample Col
Page 103 and 104:
13. The water seal of the BOD bottl
Page 105 and 106:
7.0 REFERENCES 100 - 350 350 - 500
Page 107 and 108:
14 of 14 BI - 12 Fla STE -S 5 BI -
Page 109 and 110:
Page 111 and 112:
Required Material Notes Re-order in
Page 113 and 114:
Filter Blank Filter blanks consist
Page 115 and 116:
8. When the fluorometer calls for t
Page 117 and 118:
5.2.5 Calibration Check/Laboratory
Page 119 and 120:
6. Check the calibration of the rep
Page 121 and 122:
5.3.2.2 Preparation of 100 mL of 1N
Page 123 and 124:
5.3.3.1 Procedure for Diluting Off-
Page 125 and 126:
7.0 REFERENCES APHA, AWWA, WEF. Sta
Page 127 and 128:
Chlorophyll-a Calibration Record Ca
Page 129 and 130:
Chlorophyll-a Analysis Data Sheet N
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
samples are analyzed until an accep
Page 137 and 138:
5.3.1.1 Preparation of 1000 ppm NaC
Page 139 and 140:
2. Prepare a filter housing by plac
Page 141 and 142:
2. Pipette 100 µl of the chloride
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
parts and eyes. Sulfuric Acid (H2SO
Page 149 and 150:
The available volume of each reagen
Page 151 and 152:
Corrective Action If the %D is grea
Page 153 and 154:
. Values returned by the balance sh
Page 155 and 156:
5. Shake the unfiltered sample bott
Page 157 and 158:
5.3.2.3 Sample Analysis Detailed in
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
2.0 HEALTH AND SAFETY CONSIDERATION
Page 165 and 166:
Page 167 and 168:
6 of 19 5.2.1 Method Detection Limi
Page 169 and 170:
and the samples analyzed between th
Page 171 and 172:
10 of 19 serviced and re-calibrated
Page 173 and 174:
X 20 400 X 25 500 X 50 1000 (1.0 mL
Page 175 and 176:
they must be placed in the freezer
Page 177 and 178:
Th ese instruction allow for the cr
Page 179 and 180:
6. After the computer results are p
Page 181 and 182:
USample Log Sheet SR1 SYNC (High st
Page 183 and 184:
1 of 25 Standard Operating Procedur
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Corrective Action If any method (di
Page 191 and 192:
Corrective Action If the %D is grea
Page 193 and 194:
The percent recovery is calculated
Page 195 and 196:
All phosphorus glassware should be
Page 197 and 198:
adjustable pipette to add intermedi
Page 199 and 200:
Preparation of Nitrate/Nitrite Work
Page 201 and 202:
c. Enter SPK2 for the spike number
Page 203 and 204:
Digesting Reagent for Total Phospho
Page 205 and 206:
. The standard curve is rejected if
Page 207 and 208:
Sample Log Sheet SR1 SYNC (High std
Page 209 and 210:
Standard Operating Procedure 017 Sa
Page 211 and 212:
5.2.3 Sample Replication Sample rep
Page 213 and 214:
Sample Data Sheet Analysis Tech's R
Page 215 and 216:
Salinity Analysis SOP 017 S:\WW\aww
Page 217 and 218:
Standard Operating Procedure 018 En
Page 219 and 220:
4.0 SAMPLE STORAGE, PRESERVATION AN
Page 221 and 222:
eanalyzed. The field samples will b
Page 223 and 224:
c. Dilute to the 100 mL mark on the
Page 225 and 226:
7.0 CALCULATIONS Record the number
Page 227 and 228:
11 of 21 Enterolert SOP 018 filenam
Page 229 and 230:
Page 231 and 232:
Sample Bottle Sterility Check Bacte
Page 233 and 234:
URI Watershed Watch Laboratory Quar
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Figure 1: Balance Calibration Data
Page 241 and 242:
Page 243:
6.0 DOCUMENTATION 4 of 5 Bacterial
show all

Appendix A Standard Operating Procedures - University of Rhode ...

Create successful ePaper yourself

Delete template?

Save as template?