Appendix A Standard Operating Procedures - University of Rhode ...

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4.0 DOCUMENTATION 4 of 4 Autoclave Use / Maintenance Log To be completed for EACH run. This is CRITICAL to ensure that we perform maintenance at correct intervals. If you have ANY questions, please see Elizabeth Herron (Rm 001 F, 4-4552) Date Person/lab Materials autoclaved or maintenance performed Max. Temp. (C o ) Max. Pressure (PSI) Run duration (Mins) General Autoclave Operation SOP 004 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 004 GenAutoclaveOps-MOD.doc Comments
Standard Operating Procedure 005 Bottle Autoclaving Procedure University of Rhode Island Watershed Watch 1.0 PURPOSE AND DESCRIPTION Plastic bottles must be sterilized prior to use in microbiological assays. 2.0 HEALTH AND SAFETY Date: Revision: Author: 08/08 3 Elizabeth Herron Be aware that materials that have been autoclaved will be extremely hot when the cycle is completed. Wear insulated gloves when handling materials. 3.0 AUTOCLAVE OPERATION 3.1 Preparation Clean the white HDPE plastic sampling bottles as per SOP 003 - General Labware Cleaning Procedure. Adjust the shelves of the autoclave, if necessary. Stand bottles in a metal tray, rest the caps on top of each bottle; do not engage the threads of the cap. Note: Until the bottles are sterile it doesn’t matter if the tops/lids fall on the ground. Once they have been autoclaved, you must beware of the ever-present bacteria in our surroundings and re-autoclave bottles and tops if they are opened or fall onto the ground. 3.2 Using Autoclave in the Kingston Coastal Institute Building 1. Ensure that the water reservoir is filled to the bottom of the safety value. If not, add deionized (DI) water, being sure not to immerse the safety valve. 2. Plug the power cord in, and turn the main switch to START. 3. Fill the autoclave with water by turning the multi-purpose value (always turn this valve clockwise) to FILL. The water should reach most of the width of the indicator channel at the front of the chamber. Turn the multi-purpose valve to STE to stop the flow of water and prepare the unit for sterilization. 4. Make sure that maximum temperature recording thermometer is in the autoclave. The mercury should be shaken down below 100 °C. 5. Close the door, and lock it by turning the handle clockwise. The door should be secure with a good seal, but only hand tight. 6. Set the temperature for 250 °F / 121 °C with the thermostat knob. 1 of 3 Bottle Autoclaving Procedure SOP 005 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 005 BottleAutocalve.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5 and 6: SUBJECT: Hazardous Materials and Ch
Page 7 and 8: 2.0 METHOD USED TO OBTAIN ULTRAPURE
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11 and 12: 3.2.2 Full Description of Labware C
Page 13 and 14: Standard Operating Procedure 004 Ge
Page 15: 4. Open the door and remove the loa
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21 and 22: 7. Close the door, and lock it by t
Page 23 and 24: Standard Operating Procedure 007 (P
Page 25 and 26: Several chemicals are utilized in t
Page 27 and 28: Required Material Notes Re-order in
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41 and 42: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 43 and 44: Dil. (mLs): The volume of sample fi
Page 45 and 46: Bacterial Sample Log & Worksheet: B
Page 47 and 48: Standard Operating Procedure 008 (P
Page 49 and 50: handling these chemicals. Material
Page 51 and 52: Required Material 4 of 22 Notes Re-
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
Page 57 and 58: 7. While the mTEC media is autoclav
Page 59 and 60: 12 of 22 5.3.2.3 Preparation of 1N
Page 61 and 62: 4. Label the bottom (half holding t
Page 63 and 64: 20. Transfer the plates to the 44.5
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URI Watershed Watch Laboratory Quar
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Date 22 of 22 URI Watershed Watch L
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Standard Operating Procedure 009 (P
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Disposal Samples are archived for a
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3. Aluminum weigh dishes, pans, and
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a. If a sample clogs a filter, remo
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8.0 DOCUMENTATION Example Data Shee
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4.2 pH and Alkalinity Matrix Sample
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are stored in bulk in the cabinet a
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5.3.3 Procedure - Analysis of pH on
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5. Use a non-acid washed graduated
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8.0 DOCUMENTATION Data sheet for an
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3.0 REQUIRED MATERIALS Required Mat
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field blank water and 100 mL of the
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procedure. The DO probe should not
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5.3.2 Procedure - Day of Sample Col
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13. The water seal of the BOD bottl
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7.0 REFERENCES 100 - 350 350 - 500
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14 of 14 BI - 12 Fla STE -S 5 BI -
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Required Material Notes Re-order in
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Filter Blank Filter blanks consist
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8. When the fluorometer calls for t
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5.2.5 Calibration Check/Laboratory
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6. Check the calibration of the rep
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5.3.2.2 Preparation of 100 mL of 1N
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5.3.3.1 Procedure for Diluting Off-
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7.0 REFERENCES APHA, AWWA, WEF. Sta
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Chlorophyll-a Calibration Record Ca
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Chlorophyll-a Analysis Data Sheet N
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samples are analyzed until an accep
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5.3.1.1 Preparation of 1000 ppm NaC
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2. Prepare a filter housing by plac
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2. Pipette 100 µl of the chloride
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parts and eyes. Sulfuric Acid (H2SO
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The available volume of each reagen
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Corrective Action If the %D is grea
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. Values returned by the balance sh
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5. Shake the unfiltered sample bott
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5.3.2.3 Sample Analysis Detailed in
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2.0 HEALTH AND SAFETY CONSIDERATION
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6 of 19 5.2.1 Method Detection Limi
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and the samples analyzed between th
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10 of 19 serviced and re-calibrated
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X 20 400 X 25 500 X 50 1000 (1.0 mL
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they must be placed in the freezer
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Th ese instruction allow for the cr
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6. After the computer results are p
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USample Log Sheet SR1 SYNC (High st
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1 of 25 Standard Operating Procedur
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Corrective Action If any method (di
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Corrective Action If the %D is grea
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The percent recovery is calculated
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All phosphorus glassware should be
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adjustable pipette to add intermedi
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Preparation of Nitrate/Nitrite Work
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c. Enter SPK2 for the spike number
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Digesting Reagent for Total Phospho
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. The standard curve is rejected if
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Sample Log Sheet SR1 SYNC (High std
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Standard Operating Procedure 017 Sa
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5.2.3 Sample Replication Sample rep
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Sample Data Sheet Analysis Tech's R
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Salinity Analysis SOP 017 S:\WW\aww
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Standard Operating Procedure 018 En
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4.0 SAMPLE STORAGE, PRESERVATION AN
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eanalyzed. The field samples will b
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c. Dilute to the 100 mL mark on the
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7.0 CALCULATIONS Record the number
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11 of 21 Enterolert SOP 018 filenam
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Sample Bottle Sterility Check Bacte
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URI Watershed Watch Laboratory Quar
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Figure 1: Balance Calibration Data
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6.0 DOCUMENTATION 4 of 5 Bacterial
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