Appendix A Standard Operating Procedures - University of Rhode ...

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Standard Operating Procedure 002 Laboratory Water University of Rhode Island Watershed Watch 1.0 PURPOSE AND DESCRIPTION Date: Revision: Author: 8/06 2 Linda Green There are three types of water in the URI Watershed Watch (URIWW) laboratories: tap water, deionized water and Ultrapure water. Each type of water is used for specific purposes. 1.1 Tap Water Tap water is found at each sink and is used for the initial rinsing of labware. The University of Rhode Island is located in the Kingston Water District; water is stored in the water tower on Flagg Road. 1.2 Deionized Water (DI) Deionized water (DI) is tap water that has had most ions removed. The deionizing process is done centrally for the building. Each laboratory has at least one DI water tap. In the URIWW laboratory there are two DI water taps: one at the main sink and another at the sink in the central laboratory bench. DI water is used for rinsing labware between pH and other laboratory measurements, rinsing labware after acid-soaking and for preparing certain reagents. It is obtained by turning on the tap. Do not leave the tap running. Do not leave the hose hanging in the sink when not in use, coil it up. 1.3 Ultrapure Water. Ultrapure water is made from the further processing of DI water to remove almost all ions. Each laboratory has one Aries Vaponics filtration unit. The one in the URIWW laboratory is located at the main sink. This water is sometimes referred to as “Millipore water” since that was the brand of the purifying units in Woodward Hall (the location of the URIWW laboratory prior to moving to the Coastal Institute building). This water is used for final rinsing of all critical labware, such as total phosphorus/total nitrogen (TP/TN) vials, beakers and volumetric flasks. It is also used for making up critical reagents such as nutrient standards. It should not be wasted. 1 of 3 Laboratory Water SOP 002 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 002 Lab Water-MOD.doc
2.0 METHOD USED TO OBTAIN ULTRAPURE WATER 1. Turn on the switch on the top right of the Aries Vaponics unit. 2. Observe the digital number on the front right of the unit. Once the number reaches approximately 17 megaohms the water is ready for use. a. This should only take a few seconds. b. If it takes longer contact Linda Green as the cartridges may need to be replaced. 3. Open the tap by turning the handle coming out of the right side of the unit near the hose up. 4. When making reagents let at least 500 ml water flow into the sink before collecting water for use. a. This allows the system to flush out any possible contamination collected in the hose. b. It is not necessary to allow the system to flush when rinsing labware. 5. Control the flow of water by the handle only. Do not try to slow the flow by pinching the hosing. The back pressure can damage the Aries Vaponics unit. 6. The flow of water from the unit is fairly slow; therefore the two 20 gallon carboys next to the purifying units are routinely filled with Ultrapure water for general use. a. When filling a carboy, do not forget that water is flowing and leave the laboratory. Washing the floor with Ultrapure water is expensive!!!! b. One of the carboys leaks from the valve a bit, tighten the top cap when the carboy is not in use to stop the drip. 7. Do not leave the hose from the Ultrapure unit hanging in the sink. It will pick up contaminants. Coil it up and place it over the tap when not in use. 8. Turn off the switch on the top right of the Aries-Vaptronics unit when done. 2.1 Quality Assurance/Maintenance 2.1.1 Daily Quality Assurance Each time that the Aries Vaponics unit is used the built-in digital Ohm meter will be checked. The Ohm meter should read approximately 17 megaohms. If the observed value is less than 17 megaohms contact Linda Green or Elizabeth Herron to replace the filtration cartridges. The final filter will be replaced every year. The replacement date for the filtration cartridges as well as the final filter will be noted on the cartridge and filter, respectively. 2 of 3 Laboratory Water SOP 002 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 002 Lab Water-MOD.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5: SUBJECT: Hazardous Materials and Ch
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11 and 12: 3.2.2 Full Description of Labware C
Page 13 and 14: Standard Operating Procedure 004 Ge
Page 15 and 16: 4. Open the door and remove the loa
Page 17 and 18: Standard Operating Procedure 005 Bo
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21 and 22: 7. Close the door, and lock it by t
Page 23 and 24: Standard Operating Procedure 007 (P
Page 25 and 26: Several chemicals are utilized in t
Page 27 and 28: Required Material Notes Re-order in
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41 and 42: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 43 and 44: Dil. (mLs): The volume of sample fi
Page 45 and 46: Bacterial Sample Log & Worksheet: B
Page 47 and 48: Standard Operating Procedure 008 (P
Page 49 and 50: handling these chemicals. Material
Page 51 and 52: Required Material 4 of 22 Notes Re-
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
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7. While the mTEC media is autoclav
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12 of 22 5.3.2.3 Preparation of 1N
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4. Label the bottom (half holding t
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20. Transfer the plates to the 44.5
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8.0 DOCUMENTATION Example Project D
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URI Watershed Watch Laboratory Quar
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Date 22 of 22 URI Watershed Watch L
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Standard Operating Procedure 009 (P
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Disposal Samples are archived for a
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3. Aluminum weigh dishes, pans, and
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a. If a sample clogs a filter, remo
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8.0 DOCUMENTATION Example Data Shee
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4.2 pH and Alkalinity Matrix Sample
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are stored in bulk in the cabinet a
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5.3.3 Procedure - Analysis of pH on
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5. Use a non-acid washed graduated
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8.0 DOCUMENTATION Data sheet for an
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3.0 REQUIRED MATERIALS Required Mat
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field blank water and 100 mL of the
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procedure. The DO probe should not
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5.3.2 Procedure - Day of Sample Col
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13. The water seal of the BOD bottl
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7.0 REFERENCES 100 - 350 350 - 500
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14 of 14 BI - 12 Fla STE -S 5 BI -
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Required Material Notes Re-order in
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Filter Blank Filter blanks consist
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8. When the fluorometer calls for t
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5.2.5 Calibration Check/Laboratory
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6. Check the calibration of the rep
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5.3.2.2 Preparation of 100 mL of 1N
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5.3.3.1 Procedure for Diluting Off-
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7.0 REFERENCES APHA, AWWA, WEF. Sta
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Chlorophyll-a Calibration Record Ca
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Chlorophyll-a Analysis Data Sheet N
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samples are analyzed until an accep
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5.3.1.1 Preparation of 1000 ppm NaC
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2. Prepare a filter housing by plac
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2. Pipette 100 µl of the chloride
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parts and eyes. Sulfuric Acid (H2SO
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The available volume of each reagen
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Corrective Action If the %D is grea
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. Values returned by the balance sh
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5. Shake the unfiltered sample bott
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5.3.2.3 Sample Analysis Detailed in
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2.0 HEALTH AND SAFETY CONSIDERATION
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6 of 19 5.2.1 Method Detection Limi
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and the samples analyzed between th
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10 of 19 serviced and re-calibrated
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X 20 400 X 25 500 X 50 1000 (1.0 mL
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they must be placed in the freezer
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Th ese instruction allow for the cr
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6. After the computer results are p
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USample Log Sheet SR1 SYNC (High st
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1 of 25 Standard Operating Procedur
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Corrective Action If any method (di
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Corrective Action If the %D is grea
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The percent recovery is calculated
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All phosphorus glassware should be
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adjustable pipette to add intermedi
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Preparation of Nitrate/Nitrite Work
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c. Enter SPK2 for the spike number
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Digesting Reagent for Total Phospho
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. The standard curve is rejected if
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Sample Log Sheet SR1 SYNC (High std
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Standard Operating Procedure 017 Sa
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5.2.3 Sample Replication Sample rep
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Sample Data Sheet Analysis Tech's R
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Salinity Analysis SOP 017 S:\WW\aww
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Standard Operating Procedure 018 En
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4.0 SAMPLE STORAGE, PRESERVATION AN
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eanalyzed. The field samples will b
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c. Dilute to the 100 mL mark on the
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7.0 CALCULATIONS Record the number
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11 of 21 Enterolert SOP 018 filenam
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Sample Bottle Sterility Check Bacte
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URI Watershed Watch Laboratory Quar
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Figure 1: Balance Calibration Data
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6.0 DOCUMENTATION 4 of 5 Bacterial
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