Appendix A Standard Operating Procedures - University of Rhode ...

. The other types of fecal coliform bacteria will have been turned a reddish –
purple color by being in contact with the urea substrate media.
8. Count the colonies on the remaining plates.
5.3.5 Disposal
5.3.5.1 Used Plates
Place all of the used plates into a clear autoclavable bag located in the labeled red container.
Never fill the autoclavable bags more than half full with plates. If necessary, obtain another
autoclavable bag. Follow the instructions in SOP 006 – Waste Autoclaving Procedure for using
either the Woodward Hall or the Kingston Coastal Institute autoclave to dispose of plates. Place
the cooled bag into the dumpster (do not put in a trash can as the janitors will NOT dispose of
it).
5.3.5.2 Sample Bottles
Samples may only be disposed of after final quality assurance checks are completed and the
data found to be acceptable using criteria found in Section 5.2 of this SOP. Wearing gloves,
remove the sample bottles from the refrigerator in room 019. Empty samples down the drain,
and wash bottles with hot soapy water in room 002 as discussed in SOP 003 - General Labware
Cleaning Procedure.
6.0 CALCULATIONS
Fecal coliforms and E. coli are reported in terms of the number of bacteria per 100 mL. Fecal
coliforms and E. coli counts of 0 are reported as