Appendix A Standard Operating Procedures - University of Rhode ...

7. Set the STE timer to the correct sterilization period. If the autoclave is cold set it for 55
minutes, if the autoclave is warm set for 45 minutes. Check on the unit after about 20 or
25 minutes to be sure that it is reaching the correct temperature / pressure. Small bursts
of steam may be evident from the water reservoir during the run; this is normal and
necessary. Record the unit pressure on the Autoclave Use log. It is necessary to record
the maximum pressure while the unit is running because the maximum pressure needle
is broken (as of 2008).
8. When the timer reaches 0, the run is complete.
9. Turn the multi-purpose knob to EXH + DRY.
10. When the pressure gauge reads 0, turn the main switch to STOP, and the multi-purpose
switch to 0.
11. Carefully open the door – steam will escape so do not put any body parts near the edge
of the door while opening it.
12. Allow to cool or remove the load immediately. Be sure to use insulated gloves when
handling the tray. It will be hot!
13. Record the temperature on the maximum temperature recording thermometer and the
run time on the autoclave run log (attached below). If the temperature on the recording
thermometer does not read at least 121 °C contact Elizabeth Herron.
14. Unplug the unit if you are not running any more loads immediately.
15. Do not tighten bottle caps until the bottles are cooled to room temperature or they will
warp.
16. Place a “STERILE” label over the cap so it connects to the bottle shoulder to indicate
that the bottle has been sterilized.
3.3 Using Autoclave in the Basement of Woodward Hall.
1. Seal the door by pressing the button; wait for SEALED DOOR light to come on.
2. Set EXPOSURE TIME for 20 minutes.
3. Set EXHAUST TIME for 5 minutes.
4. Select WRAPPED setting (temperature should be set for 121 C).
5. Press START.
6. The autoclave will print out a record of the sterilizing event. Write your initials, item
being sterilized, room number and phone extension on the tape (so that you can be
contacted in case there is a problem).
7. The cycle will take about ½ hour to complete. You will be unable to open the door
unless the autoclave has cooled to a safe temperature. Be sure to use insulated gloves
when handling the tray. It will be hot!
8. Once the bottles are cool to the touch, tighten the lids and put a “STERILE” label from
the cap to the shoulder of the bottle (in effect sealing it).
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Bottle Autoclaving Procedure SOP 005
S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 005 BottleAutocalve.doc