Appendix A Standard Operating Procedures - University of Rhode ...

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Date 23 of 23 URI Watershed Watch Laboratory Buffered Dilution/rinse Water KH 2PO 4 reagent pH Record Tech's Initials Meter Letter (A or B) Calibration 2 Intitial pH Volume 1N NaOH added (mL) Final pH Comments / Notes This form is intended to be used to maintain a record of pH and adjustment for the phosphate reagent for the fecal coliform dilution/rinse water. When new reagent is made up, the initial pH, amount of NaOH added, and final pH should be recorded on this sheet. Once this sheet is full it should be replaced with a new blank sheet. The full sheet should be returned to the URIWW Micro Laboratory Binder in Room 019; blank sheets are also located in this binder. Check the Laboratory QAPP, SOP 010 - Alkalinity and pH procedures, for details regarding the pH and alkalinity procedures. Calibration and Maintenance Information: 1 Each time the pH probe is used the filling solution level in the probe should be checked. The level should be within 1/4 inch of the top of the electode. If it isn't, add enough filling solution to bring it up to that point. 2 The meter should have a slope (Electrode Efficiency) of at least 96% after calibration. If the meter doesn't initially calibrate correctly, change the probe filling solution and replace the pH buffers, then recalibrate the probe. If this doesn't solve the problem replace the probe. See Linda or Elizabeth with questions. Ambient Waters Microbiological Procedure SOP 007 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 007AmbientMicrobes.doc
Standard Operating Procedure 008 (Prior number URIWW-SOP-4B) ISDS Microbiological Procedure University of Rhode Island Watershed Watch TABLE OF CONTENTS Date: Revision: Author: 08/08 3 Elizabeth Herron 1.0 PURPOSE AND DESCRIPTION ......................................................................................................1 2.0 HEALTH AND SAFETY CONSIDERATIONS..................................................................................1 2.1 HAZARDS ........................................................................................................................................1 2.2 TECHNICIAN TRAINING/QUALIFICATIONS............................................................................................2 3.0 REQUIRED MATERIALS .................................................................................................................2 4.0 SAMPLE STORAGE, PRESERVATION AND DISPOSAL .............................................................5 5.0 METHOD DESCRIPTION .................................................................................................................5 5.1 SCHEDULING CONSIDERATIONS........................................................................................................5 5.2 QUALITY ASSURANCE/QUALITY CONTROL.........................................................................................6 5.2.1 Method Detection Limit .............................................................................................................6 5.2.2 Method Blanks...........................................................................................................................6 5.2.3 Sample Replication ...................................................................................................................6 5.2.4 Positive Plates...........................................................................................................................6 5.2.5 Incubators..................................................................................................................................7 5.2.6 Germicidal Unit (UV Box) ..........................................................................................................7 5.2.7 Sterility Check on Sample Bottles.............................................................................................7 5.2.8 QA Check on New Plates..........................................................................................................8 5.2.8.1 Preparation of QC samples for new batch of plates. .......................................................................8 5.3 ANALYSIS METHOD..........................................................................................................................9 5.3.1 Preparation – 1 Week Before Scheduled Sampling..................................................................9 5.3.1.1 Preparation of mTEC Media Plates .................................................................................................9 5.3.2 Preparation - At least 48 Hours Prior to Sampling Day (As Needed) .....................................10 5.3.2.1 Preparation of 1L Sterile Phosphate Buffered Saline Solution (PBS) ............................................11 5.3.2.2 Preparation of 4 L Sterile Phosphate Buffered Saline Solution (PBS) ...........................................11 5.3.2.3 Preparation of 1N NaOH ...............................................................................................................12 5.3.2.4 Preparation of Stock KH2PO 4 solution ..........................................................................................12 5.3.2.5 Preparation of Stock MgCl2 solution ..............................................................................................12 5.3.3 Preparation - Day Before Sample Collection ..........................................................................12 5.3.4 Procedure - Day of Sample Collection....................................................................................13 5.3.4.1 Initial Preparation...........................................................................................................................13 5.3.4.2 When Samples Arrive At The Laboratory ......................................................................................13 5.3.4.3 Filtering Samples...........................................................................................................................14 5.3.4.4 Clean-up........................................................................................................................................16 5.3.4.5 Counting The Plates ......................................................................................................................16 5.3.5 Disposal...................................................................................................................................16 5.3.5.1 Used Plates ...................................................................................................................................16 5.3.5.2 Sample Bottles ..............................................................................................................................17 6.0 CALCULATIONS............................................................................................................................17 7.0 REFERENCES................................................................................................................................17 8.0 DOCUMENTATION ........................................................................................................................18 ISDS Microbiological Analysis SOP 008 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 008 ISDSMicrobes.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5 and 6: SUBJECT: Hazardous Materials and Ch
Page 7 and 8: 2.0 METHOD USED TO OBTAIN ULTRAPURE
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11 and 12: 3.2.2 Full Description of Labware C
Page 13 and 14: Standard Operating Procedure 004 Ge
Page 15 and 16: 4. Open the door and remove the loa
Page 17 and 18: Standard Operating Procedure 005 Bo
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21 and 22: 7. Close the door, and lock it by t
Page 23 and 24: Standard Operating Procedure 007 (P
Page 25 and 26: Several chemicals are utilized in t
Page 27 and 28: Required Material Notes Re-order in
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41 and 42: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 43 and 44: Dil. (mLs): The volume of sample fi
Page 45: Bacterial Sample Log & Worksheet: B
Page 49 and 50: handling these chemicals. Material
Page 51 and 52: Required Material 4 of 22 Notes Re-
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
Page 57 and 58: 7. While the mTEC media is autoclav
Page 59 and 60: 12 of 22 5.3.2.3 Preparation of 1N
Page 61 and 62: 4. Label the bottom (half holding t
Page 63 and 64: 20. Transfer the plates to the 44.5
Page 65 and 66: 8.0 DOCUMENTATION Example Project D
Page 67 and 68: URI Watershed Watch Laboratory Quar
Page 69 and 70: Date 22 of 22 URI Watershed Watch L
Page 73 and 74: Disposal Samples are archived for a
Page 75 and 76: 3. Aluminum weigh dishes, pans, and
Page 77 and 78: a. If a sample clogs a filter, remo
Page 79 and 80: 8.0 DOCUMENTATION Example Data Shee
Page 83 and 84: 4.2 pH and Alkalinity Matrix Sample
Page 85 and 86: are stored in bulk in the cabinet a
Page 87 and 88: 5.3.3 Procedure - Analysis of pH on
Page 89 and 90: 5. Use a non-acid washed graduated
Page 91 and 92: 8.0 DOCUMENTATION Data sheet for an
Page 95 and 96: 3.0 REQUIRED MATERIALS Required Mat
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field blank water and 100 mL of the
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procedure. The DO probe should not
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5.3.2 Procedure - Day of Sample Col
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13. The water seal of the BOD bottl
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7.0 REFERENCES 100 - 350 350 - 500
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14 of 14 BI - 12 Fla STE -S 5 BI -
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Standard Operating Procedure 012 (P
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Required Material Notes Re-order in
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Filter Blank Filter blanks consist
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8. When the fluorometer calls for t
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5.2.5 Calibration Check/Laboratory
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6. Check the calibration of the rep
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5.3.2.2 Preparation of 100 mL of 1N
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5.3.3.1 Procedure for Diluting Off-
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7.0 REFERENCES APHA, AWWA, WEF. Sta
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Chlorophyll-a Calibration Record Ca
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Chlorophyll-a Analysis Data Sheet N
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samples are analyzed until an accep
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5.3.1.1 Preparation of 1000 ppm NaC
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2. Prepare a filter housing by plac
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2. Pipette 100 µl of the chloride
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parts and eyes. Sulfuric Acid (H2SO
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The available volume of each reagen
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Corrective Action If the %D is grea
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. Values returned by the balance sh
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5. Shake the unfiltered sample bott
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5.3.2.3 Sample Analysis Detailed in
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2.0 HEALTH AND SAFETY CONSIDERATION
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6 of 19 5.2.1 Method Detection Limi
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and the samples analyzed between th
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10 of 19 serviced and re-calibrated
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X 20 400 X 25 500 X 50 1000 (1.0 mL
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they must be placed in the freezer
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Th ese instruction allow for the cr
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6. After the computer results are p
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USample Log Sheet SR1 SYNC (High st
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1 of 25 Standard Operating Procedur
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Corrective Action If any method (di
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Corrective Action If the %D is grea
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The percent recovery is calculated
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All phosphorus glassware should be
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adjustable pipette to add intermedi
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Preparation of Nitrate/Nitrite Work
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c. Enter SPK2 for the spike number
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Digesting Reagent for Total Phospho
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. The standard curve is rejected if
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Sample Log Sheet SR1 SYNC (High std
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Standard Operating Procedure 017 Sa
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5.2.3 Sample Replication Sample rep
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Sample Data Sheet Analysis Tech's R
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Salinity Analysis SOP 017 S:\WW\aww
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Standard Operating Procedure 018 En
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4.0 SAMPLE STORAGE, PRESERVATION AN
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eanalyzed. The field samples will b
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c. Dilute to the 100 mL mark on the
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7.0 CALCULATIONS Record the number
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11 of 21 Enterolert SOP 018 filenam
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Sample Bottle Sterility Check Bacte
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URI Watershed Watch Laboratory Quar
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Figure 1: Balance Calibration Data
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6.0 DOCUMENTATION 4 of 5 Bacterial
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