Appendix A Standard Operating Procedures - University of Rhode ...

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8.0 DOCUMENTATION Example Data Sheet Bacterial Sample Log & Worksheet: Shickasheen (8/20/04) mTEC Membrane Filtration Method Analyst - set-up: Analyst - Counts: Incubator temp. start: Waterbath temp. start: Incubator temp. end: Waterbath temp. end: Setup Dil. 24 Hr # urea Total fecal Total E.coli Monitoring Location Date (mLs) Count neg (per 100mL) (per 100mL) Mud Brook Shick - a @ Rte 2 Shick - b @ Miskiania Shick - c @ Barber Outlet Shick - d @ Rte 138 Shick @ Potter - new dam Understanding the URIWW Bacterial Data Sheet Location: The name of the waterbody or specific site from which the sample was collected. Date Setup: The date on which the sample was filtered and placed on the media. This SHOULD be the same data as the sample date (the usual hold time for samples < 6 hours). In the event that it is not the same as the sample date, the sample date should be written in parenthesis next to the location identification. 19 of 23 Ambient Waters Microbiological Procedure SOP 007 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 007AmbientMicrobes.doc
Dil. (mLs): The volume of sample filtered, reported in milliliters. Typically 100 mL are filtered, although if the bacteria levels are expected to be high smaller volumes may be filtered as well. 24 Hr Count: The number of yellow and yellow-brown colonies counted on that filter after a 24 hour total incubation period. This number corresponds to the number of fecal coliform bacteria for that volume of water. # urea neg.: The number of yellow and yellow-brown colonies counted on that filter after being placed on a urea substrate media saturated pad for 15 minutes. This number corresponds to the number of E. coli bacteria for that volume of water. Total fecal coliforms (per 100 mL): The number of fecal coliform bacteria per 100 mL (the standard method of reporting fecal coliform bacteria). This was determined by dividing the 24 Hr Count by the Dil. (mLs) and multiplying by 100. Total E. coli (per 100 mL): The number of E. coli bacteria per 100 mL (the standard method of reporting E. coli bacteria). This was determined by dividing the # urea neg. by the Dil. (mLs), and multiplying by 100. 20 of 23 Ambient Waters Microbiological Procedure SOP 007 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 007AmbientMicrobes.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5 and 6: SUBJECT: Hazardous Materials and Ch
Page 7 and 8: 2.0 METHOD USED TO OBTAIN ULTRAPURE
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11 and 12: 3.2.2 Full Description of Labware C
Page 13 and 14: Standard Operating Procedure 004 Ge
Page 15 and 16: 4. Open the door and remove the loa
Page 17 and 18: Standard Operating Procedure 005 Bo
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21 and 22: 7. Close the door, and lock it by t
Page 23 and 24: Standard Operating Procedure 007 (P
Page 25 and 26: Several chemicals are utilized in t
Page 27 and 28: Required Material Notes Re-order in
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 45 and 46: Bacterial Sample Log & Worksheet: B
Page 49 and 50: handling these chemicals. Material
Page 51 and 52: Required Material 4 of 22 Notes Re-
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
Page 57 and 58: 7. While the mTEC media is autoclav
Page 59 and 60: 12 of 22 5.3.2.3 Preparation of 1N
Page 61 and 62: 4. Label the bottom (half holding t
Page 63 and 64: 20. Transfer the plates to the 44.5
Page 65 and 66: 8.0 DOCUMENTATION Example Project D
Page 67 and 68: URI Watershed Watch Laboratory Quar
Page 69 and 70: Date 22 of 22 URI Watershed Watch L
Page 73 and 74: Disposal Samples are archived for a
Page 75 and 76: 3. Aluminum weigh dishes, pans, and
Page 77 and 78: a. If a sample clogs a filter, remo
Page 79 and 80: 8.0 DOCUMENTATION Example Data Shee
Page 83 and 84: 4.2 pH and Alkalinity Matrix Sample
Page 85 and 86: are stored in bulk in the cabinet a
Page 87 and 88: 5.3.3 Procedure - Analysis of pH on
Page 89 and 90: 5. Use a non-acid washed graduated
Page 91 and 92: 8.0 DOCUMENTATION Data sheet for an
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Standard Operating Procedure 011 (P
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3.0 REQUIRED MATERIALS Required Mat
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field blank water and 100 mL of the
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procedure. The DO probe should not
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5.3.2 Procedure - Day of Sample Col
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13. The water seal of the BOD bottl
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7.0 REFERENCES 100 - 350 350 - 500
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14 of 14 BI - 12 Fla STE -S 5 BI -
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Required Material Notes Re-order in
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Filter Blank Filter blanks consist
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8. When the fluorometer calls for t
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5.2.5 Calibration Check/Laboratory
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6. Check the calibration of the rep
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5.3.2.2 Preparation of 100 mL of 1N
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5.3.3.1 Procedure for Diluting Off-
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7.0 REFERENCES APHA, AWWA, WEF. Sta
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Chlorophyll-a Calibration Record Ca
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Chlorophyll-a Analysis Data Sheet N
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samples are analyzed until an accep
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5.3.1.1 Preparation of 1000 ppm NaC
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2. Prepare a filter housing by plac
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2. Pipette 100 µl of the chloride
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parts and eyes. Sulfuric Acid (H2SO
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The available volume of each reagen
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Corrective Action If the %D is grea
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. Values returned by the balance sh
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5. Shake the unfiltered sample bott
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5.3.2.3 Sample Analysis Detailed in
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2.0 HEALTH AND SAFETY CONSIDERATION
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6 of 19 5.2.1 Method Detection Limi
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and the samples analyzed between th
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10 of 19 serviced and re-calibrated
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X 20 400 X 25 500 X 50 1000 (1.0 mL
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they must be placed in the freezer
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Th ese instruction allow for the cr
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6. After the computer results are p
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USample Log Sheet SR1 SYNC (High st
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1 of 25 Standard Operating Procedur
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Corrective Action If any method (di
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Corrective Action If the %D is grea
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The percent recovery is calculated
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All phosphorus glassware should be
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adjustable pipette to add intermedi
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Preparation of Nitrate/Nitrite Work
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c. Enter SPK2 for the spike number
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Digesting Reagent for Total Phospho
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. The standard curve is rejected if
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Sample Log Sheet SR1 SYNC (High std
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Standard Operating Procedure 017 Sa
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5.2.3 Sample Replication Sample rep
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Sample Data Sheet Analysis Tech's R
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Salinity Analysis SOP 017 S:\WW\aww
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Standard Operating Procedure 018 En
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4.0 SAMPLE STORAGE, PRESERVATION AN
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eanalyzed. The field samples will b
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c. Dilute to the 100 mL mark on the
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7.0 CALCULATIONS Record the number
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11 of 21 Enterolert SOP 018 filenam
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Sample Bottle Sterility Check Bacte
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URI Watershed Watch Laboratory Quar
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Figure 1: Balance Calibration Data
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6.0 DOCUMENTATION 4 of 5 Bacterial
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