Appendix A Standard Operating Procedures - University of Rhode ...

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6. Set EXHAUST TIME for 5 minutes (for both trays and plates). 7. Select the UNWRAPPED setting. Temperature should be set for 132 C for both trays and plates. 8. Press START. 9. The autoclave will print out a record of the sterilizing event. Write your initials, item being sterilized, building and room number and phone extension on the tape (so that you can be contacted in case there is a problem). 10. The cycle will take about ½ hour to complete. You will be unable to open the door unless the autoclave has cooled to a safe temperature. Be sure to use the insulated gloves when handling the tray. It will be hot! 11. If you were autoclaving plates, once they are cool enough to handle, bring the waste to the dumpster and carefully discard the plates, bag and all. The media may still be liquid, so be careful not to spill any on you – it will HURT! You must bring the bags to the dumpster, the janitors will not dispose of them. 12. If you are autoclaving IDEXX trays, once they are cool enough, drain bags down the sink with running water and then discarded them in the dumpster. You must bring the bags to the dumpster, the janitors will not dispose of them. 13. Clean the autoclave tray with soap and hot water. 14. Place a new autoclave bag into the red waste container. 3 of 3 Waste Autoclaving Procedure SOP 006 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 006WasteAutoclave.doc
Standard Operating Procedure 007 (Prior number URIWW-SOP-4A) Ambient Waters Microbiological Procedure University of Rhode Island Watershed Watch TABLE OF CONTENTS Date: Revision: Author: 08/08 3 Elizabeth Herron 1.0 PURPOSE AND DESCRIPTION ......................................................................................................1 2.0 HEALTH AND SAFETY CONSIDERATIONS..................................................................................1 2.1 HAZARDS ........................................................................................................................................1 2.2 TECHNICIAN TRAINING/QUALIFICATIONS...........................................................................................2 3.0 REQUIRED MATERIALS .................................................................................................................2 4.0 SAMPLE STORAGE, PRESERVATION AND DISPOSAL .............................................................5 5.0 METHOD DESCRIPTION .................................................................................................................5 5.1 SCHEDULING CONSIDERATIONS .......................................................................................................5 5.2 QUALITY ASSURANCE/QUALITY CONTROL........................................................................................6 5.2.1 Method Detection Limit .............................................................................................................6 5.2.2 Method Blanks...........................................................................................................................6 5.2.3 Sample Replication ...................................................................................................................6 5.2.4 Positive Plates...........................................................................................................................7 5.2.5 Incubators..................................................................................................................................7 5.2.6 Germicidal Unit (UV Box) ..........................................................................................................7 5.2.7 Sterility Check on Sample Bottles.............................................................................................7 5.2.8 QA Check on New Plates..........................................................................................................8 5.2.8.1 Preparation of QC samples for new batch of plates. .......................................................................8 5.3 ANALYSIS METHOD .........................................................................................................................9 5.3.1 Preparation – 1 Week Before Scheduled Sampling..................................................................9 5.3.1.1 Preparation of mTEC Media Plates .................................................................................................9 5.3.2 Preparation - At least 48 Hours Prior to Sampling Day (As Needed) .....................................11 5.3.2.1 Preparation of 1L Sterile Phosphate Buffered Saline Solution (PBS) ............................................11 5.3.2.2 Preparation of 4 L Sterile Phosphate Buffered Saline Solution (PBS) ...........................................11 5.3.2.3 Preparation of Urea Substrate .......................................................................................................12 5.3.2.4 Preparation of 1N NaOH ...............................................................................................................12 5.3.2.5 Preparation of Stock KH2PO 4 solution ..........................................................................................12 5.3.2.6 Preparation of Stock MgCl2 solution ..............................................................................................12 5.3.3 Preparation - Day Before Sample Collection ..........................................................................13 5.3.4 Procedure - Day of Sample Collection....................................................................................13 5.3.4.1 Initial Preparation...........................................................................................................................13 5.3.4.2 When Samples Arrive At The Laboratory ......................................................................................13 5.3.4.3 Filtering Samples...........................................................................................................................14 5.3.4.4 Clean-up........................................................................................................................................16 5.3.4.5 Counting The Plates ......................................................................................................................16 5.3.5 Disposal...................................................................................................................................17 5.3.5.1 Used Plates ...................................................................................................................................17 5.3.5.2 Sample Bottles ..............................................................................................................................17 6.0 CALCULATIONS............................................................................................................................17 6.1 CALCULATION OF FECAL COLIFORMS RESULTS...............................................................................17 6.2 CALCULATION OF E. COLI RESULTS: ...............................................................................................17 7.0 REFERENCES................................................................................................................................18 8.0 DOCUMENTATION ........................................................................................................................19 Ambient Waters Microbiological Procedure SOP 007 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 007AmbientMicrobes.doc
Page 1 and 2: Appendix A Standard Operating Proce
Page 3 and 4: 4. Report ANY accidents IMMEDIATELY
Page 5 and 6: SUBJECT: Hazardous Materials and Ch
Page 7 and 8: 2.0 METHOD USED TO OBTAIN ULTRAPURE
Page 9 and 10: Standard Operating Procedure 003 Ge
Page 11 and 12: 3.2.2 Full Description of Labware C
Page 13 and 14: Standard Operating Procedure 004 Ge
Page 15 and 16: 4. Open the door and remove the loa
Page 17 and 18: Standard Operating Procedure 005 Bo
Page 19 and 20: 3 of 3 Autoclave Use / Maintenance
Page 21: 7. Close the door, and lock it by t
Page 25 and 26: Several chemicals are utilized in t
Page 27 and 28: Required Material Notes Re-order in
Page 29 and 30: 5.2 Quality Assurance/Quality Contr
Page 31 and 32: mixed it will be filtered and treat
Page 33 and 34: Procedure All equipment to come int
Page 35 and 36: 5. After completion of the autoclav
Page 37 and 38: 5. Label at least one plate from ea
Page 39 and 40: 18. After 2 hours in the incubator
Page 41 and 42: 7.0 REFERENCES APHA, AWWA & WEF. St
Page 43 and 44: Dil. (mLs): The volume of sample fi
Page 45 and 46: Bacterial Sample Log & Worksheet: B
Page 47 and 48: Standard Operating Procedure 008 (P
Page 49 and 50: handling these chemicals. Material
Page 51 and 52: Required Material 4 of 22 Notes Re-
Page 53 and 54: 5.2 Quality Assurance/Quality Contr
Page 55 and 56: 5.2.8 QA Check on New Plates E. col
Page 57 and 58: 7. While the mTEC media is autoclav
Page 59 and 60: 12 of 22 5.3.2.3 Preparation of 1N
Page 61 and 62: 4. Label the bottom (half holding t
Page 63 and 64: 20. Transfer the plates to the 44.5
Page 65 and 66: 8.0 DOCUMENTATION Example Project D
Page 67 and 68: URI Watershed Watch Laboratory Quar
Page 69 and 70: Date 22 of 22 URI Watershed Watch L
Page 71 and 72: Standard Operating Procedure 009 (P
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Disposal Samples are archived for a
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3. Aluminum weigh dishes, pans, and
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a. If a sample clogs a filter, remo
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8.0 DOCUMENTATION Example Data Shee
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Standard Operating Procedure 010 (P
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4.2 pH and Alkalinity Matrix Sample
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are stored in bulk in the cabinet a
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5.3.3 Procedure - Analysis of pH on
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5. Use a non-acid washed graduated
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8.0 DOCUMENTATION Data sheet for an
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3.0 REQUIRED MATERIALS Required Mat
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field blank water and 100 mL of the
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procedure. The DO probe should not
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5.3.2 Procedure - Day of Sample Col
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13. The water seal of the BOD bottl
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7.0 REFERENCES 100 - 350 350 - 500
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14 of 14 BI - 12 Fla STE -S 5 BI -
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Required Material Notes Re-order in
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Filter Blank Filter blanks consist
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8. When the fluorometer calls for t
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5.2.5 Calibration Check/Laboratory
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6. Check the calibration of the rep
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5.3.2.2 Preparation of 100 mL of 1N
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5.3.3.1 Procedure for Diluting Off-
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7.0 REFERENCES APHA, AWWA, WEF. Sta
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Chlorophyll-a Calibration Record Ca
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Chlorophyll-a Analysis Data Sheet N
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samples are analyzed until an accep
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5.3.1.1 Preparation of 1000 ppm NaC
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2. Prepare a filter housing by plac
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2. Pipette 100 µl of the chloride
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parts and eyes. Sulfuric Acid (H2SO
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The available volume of each reagen
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Corrective Action If the %D is grea
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. Values returned by the balance sh
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5. Shake the unfiltered sample bott
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5.3.2.3 Sample Analysis Detailed in
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2.0 HEALTH AND SAFETY CONSIDERATION
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6 of 19 5.2.1 Method Detection Limi
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and the samples analyzed between th
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10 of 19 serviced and re-calibrated
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X 20 400 X 25 500 X 50 1000 (1.0 mL
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they must be placed in the freezer
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Th ese instruction allow for the cr
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6. After the computer results are p
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USample Log Sheet SR1 SYNC (High st
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1 of 25 Standard Operating Procedur
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Corrective Action If any method (di
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Corrective Action If the %D is grea
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The percent recovery is calculated
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All phosphorus glassware should be
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adjustable pipette to add intermedi
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Preparation of Nitrate/Nitrite Work
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c. Enter SPK2 for the spike number
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Digesting Reagent for Total Phospho
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. The standard curve is rejected if
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Sample Log Sheet SR1 SYNC (High std
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Standard Operating Procedure 017 Sa
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5.2.3 Sample Replication Sample rep
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Sample Data Sheet Analysis Tech's R
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Salinity Analysis SOP 017 S:\WW\aww
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Standard Operating Procedure 018 En
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4.0 SAMPLE STORAGE, PRESERVATION AN
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eanalyzed. The field samples will b
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c. Dilute to the 100 mL mark on the
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7.0 CALCULATIONS Record the number
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11 of 21 Enterolert SOP 018 filenam
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Sample Bottle Sterility Check Bacte
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URI Watershed Watch Laboratory Quar
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Figure 1: Balance Calibration Data
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6.0 DOCUMENTATION 4 of 5 Bacterial
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