p-Tert-Butylphenol - UNEP Chemicals

OECD SIDS P-TERT-BUTYLPHENOL
110
by an occlusive patch and fixed with a bandage for 48 hours. For the
challenge treatment after 2 weeks, surgical gauze was covered with 0.2g of
the 1 % test substance in Vaseline and placed onto the clipped right flank of
the animals. An occlusive patch with the vehicle alone was applied to the left
flank. The patches were held in contact with the skin by an occlusive patch
and fixed with a bandage for 24 hours. Assessment took place 48 and 72
hours after administration.
Reference: Hüls Infracor GmbH: 1998
(b)
Type:
Species/strain: Guinea pig
Results: Sensitizing [X]; Not sensitizing [ ]; Ambiguous [ ]
Classification: Sensitizing [ ]; Not sensitizing [ ]
Method: Other
GLP: Yes [ ] No [ ] ? [X]
Test substance: purity: unknown
Remarks: 20 white female guinea pigs were painted daily on the bare skin behind their
ears with one drop of 30 % solution of a p-t-butylphenol resin in ethyl acetate
for three weeks followed by a two weeks rest. 0.5 % resin and 1 % p-tbutylphenol
in ethyl acetate was applied behind the right and left nipple,
respectively and biopsies were conducted after 48 hours. In histological
examination, 15 of 20 guinea pigs showed contact allergic reactions to the
resin. 7 of these 15 animals showed positive reactions to p-t-butylphenol and
in 8 of these 15 animals sensitization appears not to be based upon a
demonstrable sensitivity to p-t-butylphenol. Starting with an application of
30 % p-t-butylphenol behind both ears followed by application of 1 % p-tbutylphenol
and 0.5 % resin behind the right and left ear, respectively, 14
animals were sensitized with p-t-butylphenol and 9 of these also reacted to
the resin (procedure as described above).
Reference: Malten: 1967
*5.4 REPEATED DOSE TOXICITY
(a)
Species/strain: Rats/Crj:CD (SD)
Sex: Female [ ]; Male [ ]; Male/Female [ X ]; No data [ ]
Route of Administration: Oral (gavage)
Exposure period: 14 days
Frequency of treatment: Daily
Post exposure observation period: 1 day
Dose: 0 (Vehicle), 250, 500, 1,000 mg/kg/day
Control group: Yes [X]; No [ ]; No data [ ]; 0.5% aqueous methyl cellulose
Concurrent no treatment [ ]; Concurrent vehicle [X]; Historical [ ]
NOEL:
LOEL: 250 mg/kg/day
Results: At 1,000 mg/kg, the body weights were 10% lower than control at day 9, and
3/5 females and 1/5 male died up to day 9. At this time, all other survivors
were dissected but no toxic sign was observed by necropsy. Two females
continuously showed difficulty of breathing with noisy respiratory sound.
At 500 mg/kg, abnormality was only noisy respiratory sound in 3/5 animals
of both sexes. At 250 mg/kg, 1/5 female showed noisy respiratory sound.
Method: Other (Dose finding study)
GLP: Yes [ ] No [X] ? [ ]
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