p-Tert-Butylphenol - UNEP Chemicals

OECD SIDS P-TERT-BUTYLPHENOL
120
Remarks: The solvent control sites included at least one sector on the back, an ear and
one nipple.
Reference: Gellin et al.: 1979
(i)
Type: Depigmentation test
Results: p-t-Butylphenol was applied to the skin of 4 black guinea pigs for 4 - 5
days. Depigmentation occurred without preceding inflammation. Spots of
depigmented (white) skin and hairs were surrounded by a zone of
hyperpigmentation. Leukoderma was irreversible in some cases and
exhibited a tendency to progressing and spontaneous dissemination (no
further data available).
Remarks: 15 black guinea pigs were used as a control group.
Reference: Zavadsky & Khovanova: 1975
(j)
Type:
Results: p-t-Butylphenol (500 mg/kg/day) in a mixture of 5 ml propylene glycol
plus 50,000 mg polyethyleneglycol was applied on the skin of rabbits daily
for 20 weeks. There was an increase in the incidence of a capillaritis
consisting of perivascular infiltration and formation of thrombi.
Remarks:
Reference: Malten et al.: 1971 (Hara and Okumura: 1962)
(k)
Type: Biochemical investigation
Results: p-t-Butylphenol influenced the cresolase activity of tyrosinase in such a
way to elongate the induction period and suppress the reaction velocity of
this enzyme significantly. On the other hands, this chemical delayed the
reaction inactivation to increase the catecholase activity.
Remarks: The tyrosinase was prepared from potato by Kubowitz’s method and
determined by Warburg’s oxygen consumption method using p-cresol as a
substance in a medium of propylene glycol.
Reference: Malten et al.: 1971 (Nakajima and Ito: 1967, Hara and Nakajima: 1969)
(l)
Type: Biochemical investigation
Results: p-t-Butylphenol inhibited the dihydroxyphenylalanine (DOPA) oxidation
activity of epidermal tyrosinase from Rana pipiens (enzyme involved in
melanin synthesis) and Ki was estimated as 2.02 x 10 -4 mol/l. This
chemical might be also an effective competitive inhibitor of the oxidation
of tyrosine by Rana pipiens tyrosinase. Km for tyrosine and Ki for p-tbutylphenol
was estimated as 2.2 x 10 -3 mol/l and 1.95 x 10 -4 mol/l,
respectively.
Remarks:
Reference: McGuire & Hendee: 1971
(m)
Type: Chemobiokinetics general studies
Results: p-t-Butylphenol was incubated with UDP-glucuronyltransferase for 10 min
or sulfotransferase for 30 min at 37 degree C.
UDP-glucuronyltransferase; Km = 0.03 ± 0.01 mmol/l
Vmax = 4.08 ± 0.53 nmol/min/mg
Sulfotransferase; Km = 110 ± 32.5 µmol/l
Vmax = 0.58 ± 0.42 nmol/min/mg
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