p-Tert-Butylphenol - UNEP Chemicals

OECD SIDS P-TERT-BUTYLPHENOL
114
Without metabolic activation:
Precipitation conc:
Genotoxic effects: + ? -
With metabolic activation: [ ] [ ] [X]
Without metabolic activation: [ ] [ ] [X]
Method: Other
GLP: Yes [ ] No [ ] ? [X]
Test substance:
Remarks:
purity: > 95 %
Reference: Dean et al.: 1985
B. NON-BACTERIAL IN VITRO TEST
(a)
Type: Chromosomal aberration test
Type of cells used: Chinese hamster lung (CHL/IU) cell
Test method:
Solvent: Dimethylsulfoxide
Positive control: -S9, Mitomycin C; +S9, Cyclophosphamide
Dose: -S9 (continuous treatment): 0, 0.013, 0.025, 0.05 mg/ml
-S9 (short-term treatment): 0, 0.02, 0.04, 0.08 mg/ml
+S9 (short-term treatment): 0, 0.013, 0.025, 0.05 mg/ml
S-9: Rat liver, induced with phenobarbital and 5,6-benzoflavone
Metabolic activation: With [ ]; Without [ ]; With and Without [X]; No data [ ]
Plates/test: 2
Results:
Cytotoxicity conc: Without metabolic activation: (continuous treatment): 0.025 mg/ml
Without metabolic activation (short-term treatment): 0.08 mg/ml
With metabolic activation: (short-term treatment): not observed
Precipitation conc:
Genotoxic effects: clastogenicity polyploidy
+ ? - + ? -
With metabolic activation: [X] [ ] [ ] [X] [ ] [ ]
Without metabolic activation: [ ] [ ] [X] [X] [ ] [ ]
Method: Guidelines for Screening Mutagenicity Testing of Chemicals, Japan and
OECD TG 473
GLP: Yes [X] No [ ] ? [ ]
Test substance: purity: 99.9%
Remarks: Structural chromosome aberration was observed in CHL/IU cells with
exogenous metabolic activation by short-term treatment in 6.5 - 12.0 % of
cells at all concentrations studied. This chemical also induced polyploidy
with and without an exogenous metabolic activation system. The high
incidences of polyploidy cell were observed after 48 hr continuous
treatment but the response was apparent only at cytotoxic concentrations.
Other increases of polyploidy were small incidences.
Reference: MHW, Japan: 1996
(b)
Type: Chromosomal aberration test
Type of cells used: Rat lymphocytes
Test method:
Solvent: Dimethylsulfoxide
Positive control: -S9: Ethyl methanesulphonate at 500 µg/ml
+S9: Cyclophosphamide at 4.2 µg/ml
Dose: -S9 (20-hour cultures): 1.0 – 125 µg/ml
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