p-Tert-Butylphenol - UNEP Chemicals

OECD SIDS P-TERT-BUTYLPHENOL
Remarks: These enzymes were prepared from human liver.
Reference: Temellini et al.: 1991
(n)
Type: Chemobiokinetics general studies
Results: Male Sprague-Dawley rats were administered p-t-butylphenol by gavage at
a single dose of 2 and 10 mg/kg b.w. As a result, aminopyrine demethylase
activity was inhibited. Microsomal cytochrome b5 and P-450 contents were
increased 1, 12 and 24 h after administration. Although ascorbatedependent
lipid peroxidation of mitochondria and microsomes were
increased, NADPH-dependent lipid peroxidation was decreased. Swelling
and decrease of rough endoplasmic reticulum and increase of smooth
endoplasmic reticulum was observed.
Remarks:
Reference: Nakayama et al.: 1988
(o)
Type: Radioimmunoassay
Results: p-t-Butylphenol showed less than 10 % competition for the thyroxin (T4)
binding site of transthyretin at a concentration level of 15 mg/l.
Remarks:
Reference: Van den Berg et al.: 1991
(p)
Type: Cytotoxicity
Results: Hemolytic activity in rat erythrocytes was examined at 37 degree C.
Incubation period was 3 hours. As a result, the value of EC50 was 120.1
mg/l.
Remarks: Data were expressed as percentage of total hemolysis provoked by distilled
water.
Reference: Biagi et al.: 1975
(q)
Type: [ 3 H]-Thymidine incorporation study
Results: Male Syrian Golden hamsters were given diet containing p-t-butylphenol at
15 g/kg diet daily for 20 weeks, followed by single i.p. injection of 1
mCi/kg bw [methyl- 3 H]-thymidine 1 h before killing the hamsters.
Hamsters in treated group showed a significantly increased labelling index
in the forestomach, but no significant change in the pyloric region and
urinary bladder.
Remarks: For analysis of the labeling index, counts were made on 4000 cells of the
urinary bladder epithelium, 3000 cells of pyloric gland epithelium and
2000 basal cells each of the forestomach epithelium.
Reference: Hirose et al.: 1986
(r)
Type: Estrogenic activity
Results: In yeast transfected with two plasmids, one carrying human estrogen
receptor gene and the other carrying estrogen responsive element and
reporter gene Lac-Z, p-t-butylphenol indicated very weakly estrogenic
activity. The estrogenic transcriptional activity of p-t-butylphenol was 1.5
x 10 6 fold less potent than 17β-estradiol, although that of t-octylphenol was 1
x 10 3 fold less potent than 17β-estradiol.
Remarks:
Reference: Routledge & Sumpter: 1997
UNEP Publications 121