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Allelochemicals Biologica... - Name

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THE ALLELOPATHIC POTENTIAL OF GINSENOSIDES 167<br />

Figure 3. HPLC Analysis of Ginsenosides. Ginsenosides were isolated from spent<br />

broth in which either no organism (upper trace), Trichoderma hamatum (middle trace)<br />

or Pythium irregulare (lower trace) had been cultured for five days at 25 o C in the<br />

dark. Ginsenosides were chromatographed on a Microsorb-MV C-18 column (150 x<br />

4.6 mm, 5 mm) using an acetonitrile:H 2 O gradient (Nicol et al., 2002), and detected<br />

at 203 nm. The * in the lower trace indicates the unknown metabolite found in the<br />

spent broth of Py. irregulare.

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