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OSMOMAT 3000 Design and function 3.2 Measuring method basics Osmolality Freezing point depression Cosm = ΔT / K 26 Version 1.02 04/24/2013 The device measures the total osmolality of any aqueous solution. The total osmolality indicates the concentration of all osmotically active substances (such as salts, dextrose, proteins) per kilogram of water. The osmolality is specified in mOsmol/kg. The device determines the total osmolality of the sample solution based on the freezing point depression. The implemented measuring method is a relative measuring method. The freezing points of distilled water and an aqueous solution are measured and compared. The osmolality of any solution is determined using a linear function defined by 2- or 3-point calibration. The freezing point of a solution is depressed by adding soluble or mixable substances. The freezing points of pure water and a solution are measured and compared. Whereas water has a freezing point of 0°C, a solution with a saline concentration of 1 osmol/kg has a freezing point of -1.858°C. That means that one mol of a given non-dissociated substance (6.023 x 10 23 parts diluted in one kilogram of water) lowers the freezing point of a solution by 1.858°C. The following definitions are used in calculating osmolality: Cosm = osmolality [osmol/kg] T = freezing point depression [°C] K = 1.858°C kg/osmol freezing point constant
3.3 Measurement equipment Fig. 9: Overview of measurement equipment 1 Elevator 2 Upper cooling system (behind movable elevator cover) 3 Handle for lowering the thermistor probe 4 Thermistor probe 5 Lower cooling system 6 Measuring vessel 7 Cover OSMOMAT 3000 Design and function The sample is pipetted into the measuring vessel (fig. 9/7). The measuring vessel is placed on the thermistor probe (fig. 9/4) and lowered into the lower cooling system (fig. 9/5). The lower cooling system cools the sample down to a defined temperature. The defined crystallization of the sample is triggered using ice crystals produced in the upper cooling system (fig. 9/2). Based on the freezing point [°C] and the freezing point constant, the device determines the osmolality of the sample. The value is shown on the touchscreen. NOTE! Risk of incorrect measurement resulting from the presence of air bubbles in the sample! The sample must be pipetted without air bubbles. Version 1.02 04/24/2013 27
Page 1 and 2: 2/12/2013 User Guide OSMOMAT 3000 V
Page 3 and 4: OSMOMAT 3000 Overview of the OSMOMA
Page 5 and 6: Table of Contents OSMOMAT 3000 Over
Page 7 and 8: OSMOMAT 3000 Overview of the OSMOMA
Page 9 and 10: Device, rear Fig. 2: Device overvie
Page 11 and 12: Accessories Fig. 4: Overview of acc
Page 13 and 14: Special safety notices Safety notic
Page 15 and 16: 2.3.2 Risk of infection Risk of inf
Page 17 and 18: 2.3.5 Reproducibility of the measur
Page 19 and 20: Inventory OSMOMAT 3000 Safety The o
Page 21 and 22: 2.6 Personal safety gear OSMOMAT 30
Page 23 and 24: Device, rear Fig. 6: Device overvie
Page 25: Accessories Fig. 8: Overview of acc
Page 29 and 30: Thermistor probe Fig. 11: Thermisto
Page 31 and 32: Enter values Fig. 13: Enter values
Page 33 and 34: 3.6 Connections and interfaces Fig.
Page 35 and 36: 3.6.2 Transfer protocol Output form
Page 37 and 38: 4.1 Packaging About the packaging H
Page 39 and 40: 4.2 Device storage 4.3 Unpack devic
Page 41 and 42: 5.2 Install device Fig. 21: Fan out
Page 43 and 44: 6 Setup Check printer (Option D) Ch
Page 45 and 46: Select calibration standards Fig. 2
Page 47 and 48: 7.1 Power up device Fig. 31: Power
Page 49 and 50: Fig. 33: Start menu Fig. 34: Ice fo
Page 51 and 52: Fig. 40: Move elevator down Fig. 41
Page 53 and 54: Fig. 45: Ice formation Fig. 46: Cal
Page 55 and 56: Fig. 52: Clean thermistor probe 12.
Page 57 and 58: Fig. 53: Clean thermistor probe Fig
Page 59 and 60: Calibrate device using calibration
Page 61 and 62: Fig. 63: Second calibration standar
Page 63 and 64: Configure screen saver Fig. 68: Con
Page 65 and 66: 7.6 Power down device Fig. 72: Powe
Page 67 and 68: 8.2 Notes regarding errors Fig. 75:
Page 69 and 70: Measurement procedure takes longer
Page 71 and 72: Device cannot be powered up Power s
Page 73 and 74: Fig. 77: Remove mounting screw Fig.
Page 75 and 76: Check free movement of initiation n
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Fig. 86: Move elevator down Fig. 87
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Fig. 93: Remove mounting plate 1 Mo
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Fig. 99: Elevator cover with correc
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8.4.3 Replace fine-wire fuses Fig.
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Fig. 108: Pull out printer Fig. 109
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Fig. 114: Pull out printer Fig. 115
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9.1 Service table OSMOMAT 3000 Serv
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Fig. 121: Remove mounting screws Fi
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Install device front panel Fig. 127
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Fig. 131: Lubricate elevator guide
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9.5 Check reproducibility of measur
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11 Specifications 11.1 Dimensions 1
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OSMOMAT 3000 Specifications Power c
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12 Index 2 2-point calibration ....
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T Temperature sensor Replace ......
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Fig. 138: "Safe Transport" menu Fig
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Classification of the IVD OSMOMAT 3
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Before Calling Gonotec Fig. 141: Co
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