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Tree Improvement Program Project Report 2006 / 2007

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to 2000. Crosses were completed at Kalamalka breeding<br />

arboretum (KAL) in 1996 and at the Prince George<br />

arboretum in 1999. Sowing was done in March 2000 at<br />

the Skimikin Nursery, and seedlings were lifted by family<br />

and pre-designated planting design in November 2000.<br />

Plantations in Prince George(PG), one at the Prince George<br />

<strong>Tree</strong> <strong>Improvement</strong> Station (PGTIS), and the other one 30<br />

km east of PG (Wansa Creek), were established in 2001.<br />

Because we wanted to assess both male and female<br />

effects (20 each) but could not deal with a full factorial<br />

(400 crosses), we grouped the crosses into sets (five) in<br />

which four females were completely crossed with four<br />

males. All KAL crosses were completed (80 in total), but we<br />

missed two clones at PG (78). The crosses included north<br />

pollen on north females, south pollen on south females, and<br />

the two reciprocals (south on north and north on south). In<br />

setting out the field design, we used six row-tree plots and<br />

arranged the specific male x female crosses into small subblocks.<br />

We also arranged the S/S, N/N, S/N, N/S crosses<br />

for the same parents in the same place in each of four rows.<br />

Because the PG crosses were made three years after the KAL<br />

crosses (1996), we also collected OP cones from both PG<br />

and KAL in the year the crosses were made at PG (1999).<br />

Each block at Wansa creek and PGTIS are exactly the<br />

same design. Each site has five sets of identical female<br />

x male crosses completely crossed with each other (four<br />

females x four males for a total of 16 crosses/set or a total<br />

of 80 crosses per site). Each of the 16 crosses also had four<br />

parent sources (i.e., north mother x north father, south<br />

mother x south father, and the two reciprocals) for a total<br />

of 320 families per site. We planted 30 seedlings for each<br />

family as six tree-row plots in each of five blocks per site.<br />

As a result, each block had a total of 1,920 seedlings. We<br />

also planted out six OP seedlings for each of the 20 female<br />

parent trees per site (20 N/N and 20 S/S) for a total of 240<br />

seedlings per site. Thus, there are a total of 2,160 seedlings<br />

per block and a total of 10,800 seedlings per site.<br />

In the fall of 2001, we measured both first- (2000) and<br />

second-year heights (2001). Because the winter of 00/01<br />

was harsher than normal, especially at the more exposed<br />

PGTIS plantation, we also assessed winter damage.<br />

The PGTIS and WANSA plantations completed their<br />

seven-year growth in <strong>2006</strong>. The stock is about one meter<br />

tall. To determine if photoperiod differences between<br />

Prince George and Vernon affect bud flush and growth, we<br />

randomly selected four specific female x male crosses per<br />

set (a total of 20 crosses per site) including the four parent<br />

sources combination for a total of 80 crosses (80 crosses<br />

T R E E I M P R O V E M E N T P R O G R A M<br />

P R O J E C T R E P O R T 2 0 0 6 / 2 0 0 7<br />

x 6 seedlings/cross or 480 seedlings per block). Bud flush<br />

was scored categorically on each of four days. Bud flush<br />

was defined as the date when the terminal bud on any of<br />

the first-order lateral shoots burst its scales. The same 20<br />

families and 80 crosses were surveyed in each of the five<br />

blocks (2,400 seedlings) on each of the two Prince George<br />

plantation sites (a total of 4,800 seedlings). At the end of<br />

the growing season, we recorded height growth for all the<br />

stock in both the WANSA and PGTIS plantations (about<br />

20,000 seedlings).<br />

RESULTS<br />

F rst-year performance (2001)<br />

Height growth<br />

Stock was grown at (Skimikin) in 2000 and plantations<br />

were established in the spring of 2000. Fall height<br />

measurements were recorded for the first- and second-year<br />

heights. Figures 51 and 52 show the mean height growth<br />

(cm) for each of the four female x male sources. Note<br />

standard error bars are so small (+/-0.1 cm) that they do not<br />

show up in the figures).<br />

Damage assessment (2001)<br />

As we took height measurements, we assessed winter<br />

(00/01) damage. Table 22 shows the coding and number<br />

of seedlings for each class of damage for the combined<br />

assessment of both the PGTIS and WANSA plantations.<br />

Figure 53 (WANSA) and Figure 54 (PGTIS) summarize<br />

the same data graphically. In general, seedling damage to<br />

progeny from PG (PG x PG) was about 10 percent less<br />

than that from progeny from Vernon (KAL x KAL), with<br />

the damage scores for the reciprocals falling between the<br />

two.<br />

Seventh-year he ght and phenology<br />

Bud burst (PGTIS Plantation)<br />

Delayed bud flush is one of the physiological traits observed<br />

for progeny from southern seed orchards compared to their<br />

northern counterparts. Because a delay in bud flush could<br />

explain differences in growth and frost hardiness, bud flush<br />

(Julian Date) was scored for a sample of identical progeny<br />

from each of the four parent sources (i.e., KAL x KAL, KAL<br />

x PG, PG x KAL and PG x PG). Figures 55 and 56 show<br />

the Julian Date for bud burst of subtending lateral shoots.<br />

There was no difference in bud flush for progeny from<br />

each of the four parent sources.

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