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The University of -210 Nottingham M
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treated oocytes was recorded at 38
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TABLE OF CONTENTS ABSTRACT ........
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2.5.1.2 Preparation of enucleation/
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CHAPTER 6 .........................
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LIST OF FIGURES Figure 1.1 Process
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LIST OF ABBREVIATIONS A23187 AC AI
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MgSO4.7H20 MAPK MAPKK MAPKKK MBP mg
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CHAPTER 1 LITERATURE REVIEW The aim
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The production of multiple offsprin
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cytoplasm which contained the nucle
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pluripotency, as demonstrated by th
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gene-targeting via somatic cell clo
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conventional breeding and genetic m
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Oocyte maturation is generally cont
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ooýcýýommý MPF Time Figure 1.3
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not obtain clones by SCNT using bra
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A Nuclear NEBD PCC reformation DNA
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1.2.3.2 MPF, MAPK, NEBD and PCC in
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Little is known about the mechanism
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development of porcine embryos prod
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short-wavelength, UV excitable fluo
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The procedure is to expel the PBI a
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The majority of successful porcine
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Until 1997, the established dogma i
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deacetylases (HDACs). Trichostatin
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Figure 1.5 presents the general exp
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CHAPTER 2 General materials and met
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2.3 Oocyte staining and assessment
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- Page 61 and 62: ! 00-0 !".. ww "'') ^ýýe ;ý ý
- Page 63 and 64: ,rjý. 7!: rY Is All Pi D r. Figure
- Page 65 and 66: were washed three times in embryo c
- Page 67 and 68: Three replicates of batches of oocy
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- Page 71 and 72: 3.2 MATERIALS AND METHODS 3.2.1 Exp
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- Page 75 and 76: 3.3 RESULTS 3.3.1 Conventional matu
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- Page 79 and 80: Meiotic stage in CAMP treated oocyt
- Page 81 and 82: 3.4 DISCUSSION The development of N
- Page 83 and 84: 44 h. It was comparable to that (93
- Page 85 and 86: oocyte (Chen et al., 1990). In the
- Page 87 and 88: CHAPTER 4 Parthenogenetic developme
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- Page 91 and 92: COCs were collected and oocytes wer
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- Page 97 and 98: 4.4 DISCUSSION For successful porci
- Page 99 and 100: In addition to the listed benefits
- Page 101 and 102: CHAPTER 5 Effect of caffeine treatm
- Page 103 and 104: in vitro substrates. In pigs, Naito
- Page 105 and 106: 5.2 MATERIALS AND METHODS 5.2.1 Exp
- Page 107 and 108: 5.2.2 In vitro maturation of porcin
- Page 109: Blastocysts at day 7 were stained a
- Page 113 and 114: A 4500000 N 4000000 3500000 Z 30000
- Page 115 and 116: e ýo; +ý \.. 4.0 ob "i "s NW Ir
- Page 117 and 118: 5.3.4 Effects of caffeine treatment
- Page 119 and 120: Figure 5.6 Effects of 5 mM caffeine
- Page 121 and 122: 5.4 DISCUSSION In the first experim
- Page 123 and 124: In the final experiment, developmen
- Page 125 and 126: CHAPTER 6 General discussion 7.1 Ma
- Page 127 and 128: cumulus cells could be observed spe
- Page 129 and 130: parthenogenetic development excludi
- Page 131 and 132: demonstrated treatment of the TI en
- Page 133 and 134: REFERENCES Abeydeera, L. R., Wang,
- Page 135 and 136: Bromhall, J. D. (1975) Nuclear trna
- Page 137 and 138: Christmann, L., Jung, T. and Moor,
- Page 139 and 140: and human ovarian oocytes. Nature,
- Page 141 and 142: Funahashi, H., Cantley, T. C. and D
- Page 143 and 144: Hartwell, L. H. (1973) Three additi
- Page 145 and 146: Jelinkovä, L., Kubelka, M., Motlik
- Page 147 and 148: Kubelka, M., Anger, M., Pavlok, A.,
- Page 149 and 150: Le Bourhis, D., Beaujean, N., Ruffi
- Page 151 and 152: Liu, L., Oldenbourg, R., Trimarchi,
- Page 153 and 154: Minshull, J., Blow, J. J. and Hunt,
- Page 155 and 156: Oback, B. and Wells, D. N. Cloning
- Page 157 and 158: A. E., Garst, A. S., Moore, M., Dem
- Page 159 and 160: Rossomando, A. J., Payne, D. M., We
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Spemann, H. Embryonic development a
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Development. 55(2), 121-127. Törne
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Production of the first cloned came
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oocytes. Reproduction, 125(5), 645-
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00 Cl) h 'Cb a) Ü^W wv F NC a b d
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In In ý. ö O V %) ö ö N _ ö O
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