-210 Nottingham - Nottingham eTheses - The University of Nottingham
-210 Nottingham - Nottingham eTheses - The University of Nottingham
-210 Nottingham - Nottingham eTheses - The University of Nottingham
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
5.3.3 Effects <strong>of</strong> caffeine treatment on parthenogenetic<br />
development <strong>of</strong> TI and early MII oocytes<br />
At 38 hpm, denuded oocytes arrested at TI or early MII stage were cultured in<br />
maturation medium (modified NCSU-23) with different concentrations <strong>of</strong> caffeine (0,<br />
5,10 and 20 mM) for 6h and then activated. <strong>The</strong> development <strong>of</strong> the parthenotes to<br />
the blastocyst stage was recorded (Table 5.1; Figure 5.4; Figure 5.5). No statistical<br />
differences were observed in the frequency <strong>of</strong> cleavage and development to blastocyst<br />
by 0,5,10 and 20 mM caffeine treatment (chi-square, P>0.05). Also, no statistical<br />
differences were observed in the total cell number per blastocyst between 0 and 5 mM<br />
caffeine treatment and between 5 and 10 mM caffeine treatment (T test, P>0.05).<br />
However, 10 mM caffeine treatments significantly increased the total cell number<br />
compared to control and 20 mM treatment (T test, P