-210 Nottingham - Nottingham eTheses - The University of Nottingham

4.3.2 Maturation timing of cAMP treated oocytes
for TI
enucleation
Although CHX gave increased cell numbers, cAMP was selected to synchronise
oocytes based on greater expansion of cumulus cells which could be beneficial for
cytoplasmic maturation, higher efficiency of synchronising porcine IVM and higher
percentage of MII oocytes at 44 hpm and the frequency of parthenogenetic
development which did not differ from CHX treated or control groups. The
distribution of nuclear morphology of oocytes cultured in the absence or presence of 1
mM cAMP was examined at 2h intervals between 38 hpm and 44 hpm. The entire
meiotic progression of oocytes in control and cAMP group was shown in Figure 4.2,
respectively. Pictures were derived based on the data from Table 4.2 combined with
data from section 3.3.3. It was found that TI oocytes peaked at 38 hpm after cAMP
treatment.
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