-210 Nottingham - Nottingham eTheses - The University of Nottingham

pluripotency, as demonstrated by the production of induced pluripotent stem (iPS)
cells (Takahashi and Yamanaka, 2006).
SCNT is now a standard methodology in over 130 labs across 20 countries since
Dolly the sheep was born in 1996. Various types of species have now been cloned
using a range of foetal and adult derived donor cells of different cell types (Table 1.1).
About 75% of these labs are working on livestock (cattle, pig, sheep and goat), less
than 10% on mice, and the rest on at least 20 other species (Oback and Wells, 2003).
Table 1.1 Major landmarks in mammalian SCNT
Various types of species have been cloned using foetal or adult donor cells of
different cell types.
a 1st transgenic animal by SCNT
b 1st gene targeted mammal using adult somatic cells as donor nuclei
Species Donor age Cell type Reference
sheep foetal fibroblast Wilmut et al. (1997)
adult mammary epithelial Wilmut et al. (1997)
foetal Factor IX transgenic fibroblast Schnieke et al. (1997) 8
foetal gene-targeted fibroblast McCreath et al. (2000) b
cattle foetal fibroblast Cibelli et al. (1998)
adult oviduct epithelial Kato et al. (1998)
mouse adult cumulus Wakayama et al. (1998)
goat foetal fibroblast Baguisi et al. (1999)
pig adult cumulus Polejaeva et al. (2000)
gaur adult fibroblast Lanza et al. (2000)
mouflon adult granulosa Loi et al. (2001)
cat adult cumulus Shin et al. (2002)
rabbit adult cumulus Chesne et al. (2002)
rat foetal fibroblast Zhou et al. (2003)
mule foetal fibroblast Woods et al. (2003)
horse adult fibroblast Galli et al. (2003)
dog adult fibroblast Lee et al. (2005a)
ferret foetal fibroblast Li et al. (2006)
wolf adult fibroblast Kim et al. (2007)
buffalo foetal fibroblast Shi et al. (2007)
adult granulosa Shi et al. (2007)
camel adult cumulus Wani et al. (2010)
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