-210 Nottingham - Nottingham eTheses - The University of Nottingham
-210 Nottingham - Nottingham eTheses - The University of Nottingham
-210 Nottingham - Nottingham eTheses - The University of Nottingham
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Meiotic stage in CAMP treated oocytes<br />
100%<br />
80% 2 Mil<br />
70% TI<br />
60% Al<br />
ö 50%<br />
40%<br />
MI<br />
ProMl<br />
30%<br />
20%<br />
GVIV<br />
---<br />
10%<br />
GVIII<br />
0% GVII<br />
0 12 22 28 36 44 GVI<br />
Time (h)<br />
Figure 3.3 Distribution <strong>of</strong> meiotic progression (mean ± SEM) in control, CHX and<br />
cAMP treatments during maturation.<br />
At various time points oocytes were removed from culture and assessed for the stage <strong>of</strong><br />
meiotic maturation with 75-105 oocytes assessed at each time point. <strong>The</strong> classification <strong>of</strong><br />
meiotic stages is as described in Figure 3.1. In particular, control and CHX treated<br />
oocytes were mainly at the ProMI and MI stages whereas most <strong>of</strong> cAMP treated oocytes<br />
were at the GVII stage at 22 h. At 36 h, the percentage <strong>of</strong> MII oocytes <strong>of</strong> cAMP group was<br />
significantly lower than those <strong>of</strong> control and CHX groups (chi-square, p0.05) whilst<br />
the proportion <strong>of</strong> MII oocytes <strong>of</strong> CHX group was lower (chi-square, p
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